ST3GAL5 Antibody

Western Blot: ST3GAL5 Antibody [NBP2-20492] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 10% SDS PAGE gel, diluted at 1:1000.

Immunohistochemistry-Paraffin: ST3GAL5 Antibody [NBP2-20492] - Human breast cancer, using ST3GAL5 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Western Blot: ST3GAL5 Antibody [NBP2-20492] - Sample (30 ug of whole cell lysate) A: Raji 10% SDS PAGE gel, diluted at 1:1000.

• Reactivity: Hu, Mu, Rt, Bv, Pm, RM, Sh
• Applications: WB, IHC, IHC-P
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

ST3GAL5 Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human ST3GAL5. The exact sequence is proprietary.
• Localization: Golgi apparatus membrane; Single-pass type II membrane protein
• Predicted Species: Rat (90%), Sheep (94%), Rhesus Macaque (98%), Bovine (94%), Chimpanzee (98%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ST3GAL5
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 48 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Immunogen affinity purified

Alternate Names for ST3GAL5 Antibody

• 3S-T
• alpha 2,3-sialyltransferase V
• CMP-NeuAc:lactosylceramide alpha-2,3-sialyltransferase
• EC 2.4.99.9
• Ganglioside GM3 Synthase
• GM3 synthase
• lactosylceramide alpha-2,3-sialyltransferase
• SATI
• sialyltransferase 9 (CMP-NeuAc:lactosylceramide alpha-2,3-sialyltransferase;GM3 synthase)
• Sialyltransferase 9
• Siat9
• SIATGM3S
• ST3 beta-galactoside alpha-2,3-sialyltransferase 5
• ST3Gal V
• ST3GAL5
• ST3GALV
• ST3GalVSIAT9SATI

Background

Ganglioside GM3 is known to participate in the induction of cell differentiation, modulation of cell proliferation, maintenance of fibroblast morphology, signal transduction, and integrin-mediated cell adhesion. The protein encoded by this gene is a type II membrane protein which catalyzes the formation of GM3 using lactosylceramide as the substrate. The encoded protein is a member of glycosyltransferase family 29 and may be localized to the Golgi apparatus. Mutation in this gene has been associated with Amish infantile epilepsy syndrome. Transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for ST3GAL5 Antibody (NBP2-20492)(2)

Publications using NBP2-20492

• Zhang J, van der Zon G, Ma J et al. ST3GAL5-catalyzed gangliosides inhibit TGF-b-induced epithelial-mesenchymal transition via TbRI degradation The EMBO journal 2022-12-12 [PMID: 36504224] (WB, Mouse, Human)
  
  Details:
  Dilution used in WB 1:1000
• Kurcon T, Liu Z, Paradkar AV et al. miRNA proxy approach reveals hidden functions of glycosylation. Proc. Natl. Acad. Sci. U.S.A. 2015-05-26 [PMID: 26015571] (WB, Human)

Bioinformatics

• Gene Symbol: ST3GAL5
• Uniprot:
  • Q9UNP4(Human)