B3GALT4 Antibody

Images

 
Western Blot: B3GALT4 Antibody [NBP2-15533] - Sample (30 ug of whole cell lysate) A: PC-3 B: U87-MG C: SK-N-SH 10% SDS PAGE gel, diluted at 1:1000.
Immunohistochemistry-Paraffin: B3GALT4 Antibody [NBP2-15533] - Immunohistochemical analysis of paraffin-embedded Gastric ca, using antibody at 1:500 dilution.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

B3GALT4 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human B3GALT4. The exact sequence is proprietary.
Localization
Golgi apparatus membrane
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
B3GALT4
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
42 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.0), 1.0% BSA and 20% Glycerol
Preservative
0.01% Thimerosal
Purity
Immunogen affinity purified

Alternate Names for B3GALT4 Antibody

  • B3GalT4
  • b3Gal-T4
  • beta-1,3-galactosyltransferase 4
  • Beta-1,3-GalTase 4
  • beta-3-galactosyltransferase 4
  • Beta3GalT4
  • Beta3Gal-T4
  • EC 2.4.1.62
  • Galt2
  • Gal-T2
  • GALT4
  • Ganglioside galactosyltransferase
  • UDP-Gal:betaGlcNAc beta 1,3-galactosyltransferase, polypeptide 4
  • UDP-galactose:beta-N-acetyl-galactosamine-beta-1,3-galactosyltransferase

Background

This gene is a member of the beta-1,3-galactosyltransferase (beta3GalT) gene family. This family encodes type II membrane-bound glycoproteins with diverse enzymatic functions using different donor substrates (UDP-galactose and UDP-N-acetylglucosamine) and different acceptor sugars (N-acetylglucosamine, galactose, N-acetylgalactosamine). The beta3GalT genes are distantly related to the Drosophila Brainiac gene and have the protein coding sequence contained in a single exon. The beta3GalT proteins also contain conserved sequences not found in the beta4GalT or alpha3GalT proteins. The carbohydrate chains synthesized by these enzymes are designated as type 1, whereas beta4GalT enzymes synthesize type 2 carbohydrate chains. The ratio of type 1:type 2 chains changes during embryogenesis. By sequence similarity, the beta3GalT genes fall into at least two groups: beta3GalT4 and 4 other beta3GalT genes (beta3GalT1-3, beta3GalT5). This gene is oriented telomere to centromere in close proximity to the ribosomal protein S18 gene. The functionality of the encoded protein is limited to ganglioseries glycolipid biosynthesis. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol B3GALT4
Entrez
Uniprot