SSEA-1 Antibody (4E10)

Immunocytochemistry/Immunofluorescence: CD15/Lewis X Antibody (4E10) [NBP2-22179] - Immunofluorescence analysis of PC-2 cells using CD15 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Immunohistochemistry-Paraffin: CD15/Lewis X Antibody (4E10) [NBP2-22179] - Immunohistochemical analysis of paraffin-embedded human cerebellum tissues (left) and human liver cancer tissues (right) using CD15 mouse mAb with DAB staining.

ELISA: CD15/Lewis X Antibody (4E10) [NBP2-22179] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng)

• Reactivity: Hu
• Applications: ELISA, Flow, ICC/IF, IHC
• Clone: 4E10
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

SSEA-1 Antibody (4E10) Summary

• Description: Novus Biologicals Mouse SSEA-1 Antibody (4E10) (NBP2-22179) is a monoclonal antibody validated for use in IHC, ELISA, Flow and ICC/IF. Anti-SSEA-1 Antibody: Cited in 3 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Synthesized peptide of human SSEA-1.
• Localization: Cytoplasm
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: FUT4
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence 1:200-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:200-1:1000
• Application Notes: Use in FLOW reported in scientific literature (PMID: 30344930). Use in IHC-Fr reported in secitific publication PMID: 32646856
• Theoretical MW: 59 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Ascites
• Preservative: 0.03% Sodium Azide
• Purity: Unpurified

Alternate Names for SSEA-1 Antibody (4E10)

• alpha-(1,3)-fucosyltransferase
• CD15
• EC 2.4.1
• EC 2.4.1.-
• EC 2.4.1.65
• ELAM-1 ligand fucosyltransferase
• ELFT
• FCT3AELAM ligand fucosyltransferase
• fucosyltransferase 4 (alpha (1,3) fucosyltransferase, myeloid-specific)
• Fucosyltransferase 4
• Fucosyltransferase IV
• FUC-TIV
• fucT-IV
• FUTIV
• Galactoside 3-L-fucosyltransferase
• Lewis X
• LeX
• SSEA1
• SSEA-1
• stage-specific embryonic antigen 1

Background

The product of this gene transfers fucose to N-acetyllactosamine polysaccharides to generate fucosylated carbohydrate structures. It catalyzes the synthesis of the non-sialylated antigen, Lewis x (CD15).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SSEA-1 Antibody (NBP2-22179)(3)

Publications using NBP2-22179

• Chen W, Li W, Zhang Z et al. Lipocalin-2 Exacerbates Lupus Nephritis by Promoting Th1 Cell Differentiation J. Am. Soc. Nephrol. 2020-07-09 [PMID: 32646856] (IHC-Fr)
• Cuello HA, Segatori VI, Alberto M et al. Aberrant O-glycosylation modulates aggressiveness in neuroblastoma. Oncotarget. 2018-09-25 [PMID: 30344930] (FLOW, Human)
• Ellis CN, LaRocque RC, Uddin T et al. Comparative Proteomic Analysis Reveals Activation of Mucosal Innate Immune Signaling Pathways during Cholera. Infect Immun 2015-03-01 [PMID: 25561705] (IHC-P, Human)

Bioinformatics

• Gene Symbol: FUT4