SSBP1 Antibody [Unconjugated]

Western blot shows lysates of HEK293 human embryonic kidney cell line, MOLT-4 human acute lymphoblastic leukemia cell line, K562 human chronic myelogenous leukemia cell line, and UT-7 human acute myeloid leukemia cell line. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human SSBP1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6588) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for SSBP1 at approximately 18 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

SSBP1 was detected in immersion fixed HL-60 human acute promyelocytic leukemia cell line using Sheep Anti-Human SSBP1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6588) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

• Reactivity: Hu
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

SSBP1 Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human SSBP1
  Met1-Glu148
  Accession # Q04837
• Specificity: Detects human SSBP1 in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: SSBP1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 5-15 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.2 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for SSBP1 Antibody [Unconjugated]

• mitochondrial
• MtSSB
• single-stranded DNA binding protein 1
• SSBP1

Background

SSBP1 (Single-Stranded DNA –Binding Protein; also MtSSB and PWP1-interacting protein 17) is a 17-18 kDa member of the mtSSB protein family. SSBP1 should not be confused with SSB1/SOSS-B1. Although SSBP1 binds single-stranded mitochondrial DNA, it is not the product of a mitochondrial gene. Rather, it is synthesized on ribosomes in the cytosol, and undergoes transport into mitochondria via an N-terminal mitochondrial localization signal. After import, the localization motif is cleaved, allowing for homotetramerization. Upon DNA replication, SSBP1 is phosphorylated by CDK, allowing for DNA duplication. Human SSBP1 is 148 amino acids (aa) in length. It contains a cleavable mitochondrial targeting sequence (aa 1-16), an SSB domain (aa 30-141), one phosphorylation (Ser79) and one acetylation site (Lys113). There is one potential isoform that shows a 25 aa substitution for aa 135-148. Full-length human SSBP1 shares 92% aa identity with mouse SSBP1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SSBP1 Antibody (AF6588)(3)

Publications using AF6588

• Katharina Z, Anna L, Nicolas N et al. Temporal Analysis of Protein Ubiquitylation and Phosphorylation During Parkin-Dependent Mitophagy. Mol Cell Proteomics. 2021-12-30 [PMID: 34974192]
• Katharina Z, Anna L, Nicolas N et al. Temporal Analysis of Protein Ubiquitylation and Phosphorylation During Parkin-Dependent Mitophagy. Mol Cell Proteomics. 12/30/2021 [PMID: 34974192]
• C Piro-Mégy, E Sarzi, A Tarrés-Sol, M Péquignot, F Hensen, M Quilès, G Manes, A Chakrabort, A Sénéchal, B Bocquet, C Cazevieill, A Roubertie, A Müller, M Charif, D Goudenège, G Lenaers, H Wilhelm, U Kellner, N Weisschuh, B Wissinger, X Zanlonghi, C Hamel, JN Spelbrink, M Solà, C Delettre Dominant mutations in mtDNA maintenance gene SSBP1 cause optic atrophy and foveopathy J. Clin. Invest., 2020-01-02;0(0):. 2020-01-02 [PMID: 31550237] (Western Blot, Human)

Bioinformatics

• Gene Symbol: SSBP1
• Uniprot:
  • Q04837()