SSBP1 Antibody [Alexa Fluor® 532]

• Reactivity: Hu
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor 532

SSBP1 Antibody [Alexa Fluor® 532] Summary

• Immunogen: E. coli-derived recombinant human SSBP1
  Met1-Glu148
  Accession # Q04837
• Specificity: Detects human SSBP1 in direct ELISAs and Western blots.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for SSBP1 Antibody [Alexa Fluor® 532]

• mitochondrial
• MtSSB
• single-stranded DNA binding protein 1
• SSBP1

Background

SSBP1 (Single-Stranded DNA –Binding Protein; also MtSSB and PWP1-interacting protein 17) is a 17-18 kDa member of the mtSSB protein family. SSBP1 should not be confused with SSB1/SOSS-B1. Although SSBP1 binds single-stranded mitochondrial DNA, it is not the product of a mitochondrial gene. Rather, it is synthesized on ribosomes in the cytosol, and undergoes transport into mitochondria via an N-terminal mitochondrial localization signal. After import, the localization motif is cleaved, allowing for homotetramerization. Upon DNA replication, SSBP1 is phosphorylated by CDK, allowing for DNA duplication. Human SSBP1 is 148 amino acids (aa) in length. It contains a cleavable mitochondrial targeting sequence (aa 1-16), an SSB domain (aa 30-141), one phosphorylation (Ser79) and one acetylation site (Lys113). There is one potential isoform that shows a 25 aa substitution for aa 135-148. Full-length human SSBP1 shares 92% aa identity with mouse SSBP1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.