Publications for SRPX2 Antibody (NBP1-86999)(1)
We have publications tested in 1 confirmed species: Human.
We have publications tested in 2 applications: IHC-P, WB.
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Publication using NBP1-86999
Liu KL, Wu J, Zhou Y, Fan JH. Increased Sushi repeat-containing protein X-linked 2 is associated with progression of colorectal cancer Med. Oncol. 2015 Apr 01 [PMID: 25737434] (WB, IHC-P, Human) Details: SRPX2 antibody used for WB (1:500 dilution) and IHC-P application. WB performed on lysates of colorectal cancer cell lines namely HCT116, SW480, and HT29 cells - the antibody generated specific bands at 120 kDa representing SRPX2 /Sushi repeat-containing protein X-linked 2 and the highest expression was observed in HCT116 cells (Fig. 2A). WB also performed on lysates of HCT116 cells subjected or not to transfection using siRNA- SRPX2 (Fig. 3b). IHC-P performed on colon cancer specimens and normal tissue TMAs - antigen retrieval performed by heating sections in citrate buffer pH 6.0 at 121C for 20 minutes, primary antibody used at 1:100 dilution for ON at 4C, detection carried out using EnVision+ /HRP - DAB method (Fig. 1). WB, IHC-P
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