Recombinant Human SRGN His Protein

SDS-Page: Recombinant Human SRGN Protein [NBP1-72483] - 15% SDS-PAGE (3 ug).

• Reactivity: Hu
• Applications: PAGE
• Concentration: 1 mg/ml

Recombinant Human SRGN His Protein Summary

• Description: A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 28-158 of Human SRGN
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMYPTRR ARYQWVRCNP DSNSANCLEE KGPMFELLPG ESNKIPRLRT DLFPKTRIQD LNRIFPLSED YSGSGFGSGS GSGSGSGSGF LTEMEQDYQL VDESDAFHDN LRSLDRNLPS DSQDLGQHGL EEDFML

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: SRGN
• Purity: >90%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 17.4 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH 8.0), 10% glycerol, 2 mM DTT, 0.15 M NaCl
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: >90%, by SDS-PAGE

Alternate Names for Recombinant Human SRGN His Protein

• FLJ12930
• Hematopoetic proteoglycan core protein
• MGC9289
• P.PG
• Platelet proteoglycan core protein
• platelet proteoglycan protein core
• PPG
• PRG1p.PG
• PRGhematopoetic proteoglycan core peptide
• proteoglycan 1, secretory granule
• proteoglycan protein core for mast cell secretory granule
• secretory granule proteoglycan 1
• secretory granule proteoglycan core peptide
• Secretory granule proteoglycan core protein
• serglycin proteoglycan
• Serglycin
• SRGN

Background

SRGN is a protein best known as a hematopoietic cell granule proteoglycan. Proteoglycans stored in the secretory granules of many hematopoietic cells also contain a protease-resistant peptide core, which may be important for neutralizing hydrolytic enzymes. This protein was found to be associated with the macromolecular complex of granzymes and perforin, which may serve as a mediator of granule-mediated apoptosis. Two transcript variants, only one of them protein-coding, have been found for this gene. Recombinant human SRGN protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: SRGN