SRD5A2 Antibody

Western Blot: SRD5A2 Antibody [NB300-981] - Staining of Human Prostate lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Hamster
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

SRD5A2 Antibody Summary

• Immunogen: Peptide with sequence QVQCQQSPVLAG corresponding to N-Terminus according to NP_000339.2.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Hamster
• Gene: SRD5A2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:8000
  • Western Blot 0.3-1 ug/ml
• Application Notes: WB: Approx 26kDa band observed in Human Prostate and Breast lysates (calculated MW of 28.4kDa according to NP_000339.2).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for SRD5A2 Antibody

• 3-oxo-5-alpha-steroid 4-dehydrogenase 2,5 alpha-SR2
• 5 alpha-SR2
• EC 1.3.99.5
• MGC138457
• S5AR 2
• SR type 2
• SRD5A2
• Steroid 5-alpha-reductase 2,3-oxo-5 alpha-steroid 4-dehydrogenase 2
• steroid-5-alpha-reductase, alpha polypeptide 2 (3-oxo-5 alpha-steroid delta4-dehydrogenase alpha 2)
• Type II 5-alpha reductase

Background

This gene encodes a microsomal protein expressed at high levels in androgen-sensitive tissues such as the prostate. The encoded protein is active at acidic pH and is sensitive to the 4-azasteroid inhibitor finasteride. Deficiencies in this gene can result in male pseudohermaphroditism, specifically pseudovaginal perineoscrotal hypospadias (PPSH).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SRD5A2 Antibody (NB300-981)(1)

Publication using NB300-981

• Iczkowski KA, Qiu J, Qian J et al. The dual 5-alpha-reductase inhibitor dutasteride induces atrophic changes and decreases relative cancer volume in human prostate. Urology 2005-01-01 [PMID: 15667867]

Bioinformatics

• Gene Symbol: SRD5A2
• Entrez:
  • 6716(Human)
• Uniprot:
  • NP_000339.(Human)