Recombinant Human sRank Receptor/TNFRSF11A Protein

SDS-Page: Human sRank Receptor/TNFRSF11A Protein [NBP2-35272]

• Reactivity: Hu
• Applications: PAGE, Bioactivity
• Format: Carrier-Free
• Concentration: LYOPH

Recombinant Human sRank Receptor/TNFRSF11A Protein Summary

• Description: A single non-glycosylated polypeptide chain containing 174 amino acids corresponding to sRank Receptor/TNFRSF11A Source: E. coli

  Uniprot ID: Q9Y6Q6

  Amino Acid Sequence: QIAPPCTSEK HYEHLGRCCN KCEPGKYMSS KCTTTSDSVC LPCGPDEYLD SWNEEDKCLL HKVCDTGKAL VAVVAGNSTT PRRCACTAGY HWSQDCECCR RNTECAPGLG AQHPLQLNKD TVCKPCLAGY FSDAFSSTDK CRPWTNCTFL GKRVEHHGTE KSDAVCSSSL PARK

• Details of Functionality: sRank Receptor/TNFRSF11A protein is fully biologically active when compared to standard. The ED50 as determined by its ability to inhibit sRANK Ligand induced nuclear factor kappa B(NFkB) in RAW 264.7 cells is less than 50 ng/ml, corresponding to a specific activity of > 2.0 x 10^4 IU/mg in the presence of 15 ng/ml of recombinant sRANK Ligand.
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: TNFRSF11A
• Purity: > 98 % pure by SDS-PAGE and HPLC
• Endotoxin Note: Less than 0.1 EU/ug of sRank Receptor/TNFRSF11A as determined by LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • SDS-Page
• Theoretical MW: 19.1 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered concentrated solution in 20 mM Tris-HCl, pH 8.0, 150mM NaCl.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: > 98 % pure by SDS-PAGE and HPLC
• Reconstitution Instructions: Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.

Alternate Names for Recombinant Human sRank Receptor/TNFRSF11A Protein

• CD265 antigen
• CD265
• FEO
• loss of heterozygosity, 18, chromosomal region 1
• OFE
• OPTB7
• Osteoclast differentiation factor receptor
• PDB2
• Receptor activator of NF-KB
• receptor activator of nuclear factor-kappa B
• TRANCER
• tumor necrosis factor receptor superfamily member 11A
• tumor necrosis factor receptor superfamily, member 11a, activator of NFKB
• tumor necrosis factor receptor superfamily, member 11a, NFKB activator

Background

RANK Ligand and RANK Receptor are members of the TNF superfamily that play an important role in the regulation of specific immunity and bone turnover. RANK Receptor was originally identified as a dendritic-cell-membrane protein, which by interacting with RANKL augments the ability of dendritic cells to stimulate naive T cell proliferation and to promote the survival of RANK + T cells. RANK is also expressed in a variety of tissues including skeletal muscle, thymus, liver, colon, small intestine and adrenal gland. The RANK/RANKL interaction is important in the regulation of osteoclastogenesis and in dendritic-cellmediated T cell immune responses. Impairments in RANK signaling have been implicated in the induction of expansile osteolysis and Paget disease of bone (PDB2). Recombinant human sRANK receptor is a 19.1 kDa polypeptide containing the TNFR homologous cysteine rich portion of the extracellular domain of RANK receptor (174 amino acid residues).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: TNFRSF11A
• Uniprot:
  • Q9Y6Q6()
  • Q9Y6Q6()
  • Q9Y6Q6()