Recombinant Human sRank Receptor/TNFRSF11A Protein

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SDS-Page: Human sRank Receptor/TNFRSF11A Protein [NBP2-35272]

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Func, PAGE
Format
Carrier-Free
Concentration
LYOPH

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Recombinant Human sRank Receptor/TNFRSF11A Protein Summary

Description
A single, non-glycosylated biologically active polypeptide chain corresponding to 174 residues of TNFRSF11A.

Source: E. coli

Amino Acid Sequence: QIAPPCTSEK HYEHLGRCCN KCEPGKYMSS KCTTTSDSVC LPCGPDEYLDSWNEEDKCLL HKVCDTGKAL VAVVAGNSTT PRRCACTAGY HWSQDCECCRRNTECAPGLG AQHPLQLNKD TVCKPCLAGY FSDAFSSTDK CRPWTNCTFLGKRVEHHGTE KSDAVCSSSL PARK

Preparation
Method
Novus' biologically active proteins are stringently purified to provide only the safest and most highly effective proteins available. This protein was expressed in E. coli, purified by HPLC, QC tested by SDS-PAGE and Western Blot and validated on appropriate cell lines for bioactivity. All HPLC and bioactivity data is provided for your assurance.
Details of Functionality
RANK Protein is fully biologically active when compared to standard. The ED50 as determined by its ability to inhibitsRANK Ligand induced nuclear factor kappa B(NFkappaB) in RAW 264.7 cells is less than 50 ng/ml, corresponding to a specific activity of > 2.0 x 10^4 IU/mg in the presence of 15 ng/ml of recombinant sRANK Ligand.
Source
E. coli
Protein/Peptide Type
Biologically Active Protein
Gene
TNFRSF11A
Purity
> 98 % pure by SDS-PAGE and HPLC
Endotoxin Note
Less than 0.1 EU/ug of endotoxin as determined by LAL method.

Applications/Dilutions

Theoretical MW
19.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.
Buffer
Lyophilized from a 0.2 um filtered concentrated solution in 20 mM Tris-HCl, pH 8.0, 150mM NaCl.
Concentration
LYOPH
Purity
> 98 % pure by SDS-PAGE and HPLC
Reconstitution Instructions
Reconstitute with sterilized distilled water or 0.1% BSA aqueous buffer to a final concentration of 0.1 - 1.0 mg/ml.

Notes

This lyophilized preparation is stable at 2-8 degrees C, but should be kept at -20 degrees C for long term storage, preferably desiccated. Upon reconstitution, the preparation is most stable at -20 to -80 degrees C, and can be stored for one week at 2-8 degrees C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degrees C to -80 degrees C. Avoid repeated freeze/thaw cycles.

Alternate Names for Recombinant Human sRank Receptor/TNFRSF11A Protein

  • CD265 antigen
  • CD265
  • FEO
  • loss of heterozygosity, 18, chromosomal region 1
  • OFE
  • OPTB7
  • Osteoclast differentiation factor receptor
  • PDB2
  • Receptor activator of NF-KB
  • receptor activator of nuclear factor-kappa B
  • TRANCER
  • tumor necrosis factor receptor superfamily member 11A
  • tumor necrosis factor receptor superfamily, member 11a, activator of NFKB
  • tumor necrosis factor receptor superfamily, member 11a, NFKB activator

Background

RANK Ligand and RANK Receptor are members of the TNF superfamily that play an important role in the regulation of specific immunity and bone turnover. RANK Receptor was originally identified as a dendritic-cell-membrane protein, which by interacting with RANKL augments the ability of dendritic cells to stimulate naive T cell proliferation and to promote the survival of RANK + T cells. RANK is also expressed in a variety of tissues including skeletal muscle, thymus, liver, colon, small intestine and adrenal gland. The RANK/RANKL interaction is important in the regulation of osteoclastogenesis and in dendritic-cellmediated T cell immune responses. Impairments in RANK signaling have been implicated in the induction of expansile osteolysis and Paget disease of bone (PDB2). Recombinant human sRANK receptor is a 19.1 kDa polypeptide containing the TNFR homologous cysteine rich portion of the extracellular domain of RANK receptor (174 amino acid residues).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 2 years from date of receipt.

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sRank Receptor/TNFRSF11A NBP2-35272

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Bioinformatics

Gene Symbol TNFRSF11A
Uniprot