SR-BI Antibody

Western Blot: SR-BI Antibody [NB400-131] - Analysis of SRBI in 1. human liver lysate, 2. mouse liver lysate and 3. rat liver lysate.

Immunocytochemistry/Immunofluorescence: SR-BI Antibody [NB400-131] - SRB1 antibody was tested in NIH/3T3 cells with Dylight 488 (green). Nuclei were counterstained with DAPI (blue).

Product Discontinued

SR-BI Antibody Summary

• Immunogen: A synthetic peptide made to the C-terminal region of mouse SR-BI (between residues 400-509). [UniProt# Q61009]
• Localization: Cell Membrane; multi-pass membrane protein
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: SCARB1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence 1:50
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500
• Application Notes: Flow Cytometry was reported in scientific literature. Use in IHC-Paraffin reported in scientific literature (PMID 24814604). Use in IHC reported in scientific literature (PMID: 28360088). The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. Use in IHC-Frozen reported in scientific literature (PMID 26865459).
• Theoretical MW: 82 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  Mouse Liver Whole Tissue Lysate (Adult Whole Normal)

Reactivity Notes

Customers have also reported positive detection in cytosolic fractions from 13-lined ground squirrel liver samples, with an unidentified non-specific band at ~60 kDa.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 3 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for SR-BI Antibody

• CD36 and LIMPII analogous 1
• CD36 antigen (collagen type I receptor, thrombospondin receptor)-like 1
• CD36 antigen
• CD36L1
• CD36L1scavenger receptor class B type 1
• CLA1 CD36 antigen-like 1
• CLA-1 HDLQTL6
• CLA1
• Collagen type I receptor, thrombospondin receptor-like 1
• HDLQTL6
• MGC138242
• SCARB1
• scavenger receptor class B type III
• scavenger receptor class B, member 1
• SRB1 scavenger receptor class B member 1
• SR-B1
• SRBI
• SR-BI

Background

SR-BI (scavenger receptor class B member 1, SCARB1) belongs to the CD36 family and acts as a cell surface lipoprotein receptor for a variety of ligands including high density lipoprotein (HDL), phospholipids, phosphatidylserine, and lipoproteins such as lipopolysaccharide (LPS). SR-BI mediates selective uptake of HDL cholesteryl (HDL-C) ester in the liver, steroidogenic tissues, and adipose tissue and facilitates the flux of free and esterified cholesterol between the cell surface and apoB-containing lipoproteins and modified lipoproteins. SR-BI plays a role in antigen capture and cross-presentation from bacteria, parasites, and viruses such as Hepatitis C. In endothelial cells and macrophages, SR-BI is involved in anti-inflammatory and pro-survival signaling, providing protection against atherosclerosis and clearing apoptotic cells (efferocytosis) (1, 2).

At least 5 different human splice variants have been identified with theoretical molecular weights ranging from 60.9 kDa for the canonical sequence to 45 kDa for an N-terminally truncated variant (SR-BII) (3). The extracellular domain of human SR-BI (CLA-1) shares 80%, 80%, 89%, 86% and 84% aa sequence identity with mouse, rat, porcine, rabbit, and bovine SR-BI, respectively. SR-BI is highly glycosylated and removal of N-linked glycosylation reduces lipid transport.

References

1. Linton MF, Tao H, Linton EF, Yancey PG. (2017) SR-BI: A Multifunctional Receptor in Cholesterol Homeostasis and Atherosclerosis. Trends Endocrinol Metab. 28(6):461-472. PMID: 28259375

2. Hoekstra M, Sorci-Thomas M. (2017) Rediscovering scavenger receptor type BI: surprising new roles for the HDL receptor. Curr Opin Lipidol. 28(3):255-260. PMID: 28301373

3. Shen WJ, Asthana S, Kraemer FB, Azhar S. (2018) Scavenger receptor B type 1: expression, molecular regulation, and cholesterol transport function.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SR-BI Antibody (NB400-131)(22)

Publications using NB400-131

• Yalcinkaya M, Kerksiek A, Gebert K et al. HDL inhibits endoplasmic reticulum stress-induced apoptosis of pancreatic beta-cells in vitro by activation of Smoothened J. Lipid Res. 2020-01-06 [PMID: 31907205] (WB, Rat)
• Velagapudi s, Piemontese A, Meier R et al. VEGF-A Regulates Cellular Localization of SR-BI as Well as Transendothelial Transport of HDL but Not LDL. circres-submit.aha-journals.org. 2016-09-30 [PMID: 28360088] (IF/IHC, Human)
• Ganesan LP, Mates JM, Cheplowitz AM et al. Scavenger receptor B1, the HDL receptor, is expressed abundantly in liver sinusoidal endothelial cells. Sci Rep. 2016-02-11 [PMID: 26865459] (IHC-Fr, WB, Mouse)
• Huang CX, Zhang YL, Wang Jf et al. MCP-1 impacts RCT by repressing ABCA1, ABCG1, and SR-BI through PI3K/Akt posttranslational regulation in HepG2 cells. J Lipid Res 2013-05-01 [PMID: 23402987]
• Dikkers A, de Boer JF, Annema W et al. Scavenger receptor BI and ABCG5/G8 differentially impact biliary sterol secretion and reverse cholesterol transport in mice. Hepatology 2013-02-11 [PMID: 23401258] (WB, Mouse)
• Storey SM, McIntosh AL, Huang H et al. Intracellular cholesterol-binding proteins enhance HDL-mediated cholesterol uptake in cultured primary mouse hepatocytes Am J Physiol Gastrointest Liver Physiol 2012-04-15 [PMID: 22241858] (WB, ICC/IF, Mouse)
• de Boer JF, Annema W, Schreurs M et al. Type I diabetes mellitus decreases in vivo macrophage-to-feces reverse cholesterol transport despite increased biliary sterol secretion in mice. Journal of Lipid Research. 2011-12-18 [PMID: 22180634]
• Dong P, Xie T, Zhou X, et al. Induction of macrophage scavenger receptor type BI expression by tamoxifen and 4-hydroxytamoxifen Atherosclerosis 2011-10-01 [PMID: 21820658]
• Gouveia V, Rizzello L, Vidal B et al. Targeting Macrophages and Synoviocytes Intracellular Milieu to Augment Anti Inflammatory Drug Potency Advanced Therapeutics 2022-01-10 (WB, Human)
• Velagapudi S, Schraml P et al. Scavenger receptor BI promotes cytoplasmic accumulation of lipoproteins in clear-cell renal cell carcinoma. J Lipid Res 2018-01-11 [PMID: 30173145] (WB, Human)
• Wang D, Rohrer L, von Eckardstein A Sphingosine-1-phosphate receptors 1 and 3 regulate the expression of SR-B1 in human aortic endothelial cells bioRxiv 2020-01-01
• Caron Jerome, Pene Veronique, Tolosa Laia et al. Low-density lipoprotein receptor-deficient hepatocytes differentiated from induced pluripotent stem cells allow familial hypercholesterolemia modeling, CRISPR/Cas-mediated genetic correction, and productive hepatitis C virus infection. Stem Cell Research & Therapy 2019-07-29 [PMID: 31358055] (ICC/IF)
• Oldoni F, van Capelleveen JC, Dalila N. Naturally Occurring Variants in LRP1 (Low-Density Lipoprotein Receptor-Related Protein 1) Affect HDL (High-Density Lipoprotein) Metabolism Through ABCA1 (ATP-Binding Cassette A1) and SR-B1 (Scavenger Receptor Class B Type 1) in Humans Arteriosclerosis, Thrombosis, and Vascular Biology 2018-01-01 [PMID: 29853565] (WB, Human)
• Airanthi M, Widjaja-Adhi K, Lobo GP et al. A genetic dissection of intestinal fat-soluble vitamin and carotenoid absorption Hum. Mol. Genet. 2015-02-20 [PMID: 25701869] (WB, Mouse)
  
  Details:
  SR-BI/SCARB1 antibody used for WB on mouse small intestine tissue extracts employing a primary antibody dilution of 1:1,000 with overnight incubation at 4C (Figure 6 & 7).
• Varatharajalu R, Garige M, Leckey LC et al. Adverse Signaling of Scavenger Receptor Class B1 and PGC1s in Alcoholic Hepatosteatosis and Steatohepatitis, and Protection by Betaine in Rat. Am. J. Pathol. 2014-05-06 [PMID: 24814604] (IHC-P, WB, Rat)
• Akita N, Tsujita M, Yokota T et al. High density lipoprotein turnover is dependent on peroxisome proliferator-activated receptor alpha in mice. J Atheroscler Thromb 2010-11-27 [PMID: 20668363] (Mouse)
• Liang H, Russell RS, Yonkers NL et al. Differential Effects of Hepatitis C Virus JFH1 on Human Myeloid Plasmacytoid Dendritic Cells. J Virol;83(11):5693-5707. 2009-01-01 [PMID: 19297478] (FLOW, Human)
• Robichaud JC, van der Veen JN, Yao Z et al. Hepatic uptake and metabolism of phosphatidylcholine associated with high density lipoproteins. Biochim Biophys Acta 2009-06-01 [PMID: 19250958] (WB, Mouse)
• Hashimoto K, Suzuki H, Taniguchi K et al. Catalytic mechanism of nitrile hydratase proposed by time-resolved X-ray crystallography using a novel substrate, tert-butylisonitrile. J Biol Chem 2008-12-26 [PMID: 18948265]
• Robichaud JC, Francis GA, Vance DE. A role for hepatic scavenger receptor class B, type I in decreasing high density lipoprotein levels in mice that lack phosphatidylethanolamine N-methyltransferase. J Biol Chem 2008-12-19 [PMID: 18842588]
• Akpovi, CD et al. The predominance of one of the SR-BI isoforms is associated with increased esterified cholesterol levels not apoptosis in mink testis. J Lipid Res;47(10):2233-47. 2006-10-01 [PMID: 16861621] (WB, ICC/IF, Mustelid)
• Bocharov, AV et al. Targeting of scavenger receptor class B type I by synthetic amphipathic alpha-helical-containing peptides blocks lipopolysaccharide (LPS) uptake and LPS-induced pro-inflammatory cytokine responses in THP-1 monocyte cells. J Biol Chem;279(34):36072-82. 2004-08-20 [PMID: 15199068] (ICC/IF, Human)

Reviews for SR-BI Antibody (NB400-131) (2)

Average Rating: 5

(Based on 2 reviews)

We have 2 reviews tested in 2 species: Human, Mouse.

reviewed by: Verified Customer WB Human 11/23/2016

Summary

• Application: Western Blot
• Sample Tested: Human aortic endothelial cell lysate in RIPA buffer
• Species: Human
• Lot: C1, D1

reviewed by: Verified Customer WB Mouse 03/25/2013

Summary

• Application: Western Blot
• Sample Tested: Mouse liver and small intestine
• Species: Mouse
• Lot: B1

Bioinformatics

• Gene Symbol: SCARB1