![Immunoprecipitation: Spt6 Antibody [NB100-2584] - Detection of Human SUPT6H by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-SUPT6H antibodies NB100-2583, NB100-2584 and another SUPT6H Ab used for IP at 3 mcg/mg lysate (A and B). Detection: Chemiluminescence with exposure times of 30 seconds (A and B). * - The asterisk marks a protein with which BL3468 interacts in IP and WB. This band is likely to be a cross-reacting protein.](http://images.novusbio.com/fullsize/Spt6-Antibody-Immunoprecipitation-NB100-2584-img0002.jpg "Immunoprecipitation: Spt6 Antibody [NB100-2584] - Detection of Human SUPT6H by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-SUPT6H antibodies NB100-2583, NB100-2584 and another SUPT6H Ab used for IP at 3 mcg/mg lysate (A and B). Detection: Chemiluminescence with exposure times of 30 seconds (A and B). * - The asterisk marks a protein with which BL3468 interacts in IP and WB. This band is likely to be a cross-reacting protein.")
| Reactivity | HuSpecies Glossary |
| Applications | IP, WB (-) |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1.0 mg/ml |
| Description | Novus Biologicals Rabbit Spt6 Antibody - BSA Free (NB100-2584) is a polyclonal antibody validated for use in IP. Anti-Spt6 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee. |
| Immunogen | The immunogen recognized by this antibody maps to a region between residue 1553 and the C-terminus (residue 1583) of human Suppressor of Ty 6 Homolog using the numbering given in entry NP_003161.2 (GeneID 6830). |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | SUPT6H |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | NB100-2582 or NB100-2583 are recommended for Western Blot. |
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| Storage | Store at 4C. Do not freeze. |
| Buffer | Tris-Citrate/Phosphate (pH 7.0 - 8.0) |
| Preservative | 0.09% Sodium Azide |
| Concentration | 1.0 mg/ml |
| Purity | Immunogen affinity purified |
| Publication using NB100-2584 | Applications | Species |
|---|---|---|
| Cygnar D, Hagemeier S, Kronemann D et al. The cellular protein SPT6 is required for efficient replication of human cytomegalovirus. J Virol 2012-02-01 [PMID: 22171252] |
Secondary Antibodies |
Isotype Controls |
Research Areas for Spt6 Antibody (NB100-2584)Find related products by research area.
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Breast cancer stem cells survive chemotherapy through S100A10-ANXA2-SPT6 interaction that epigenetically promotes OCT4-mediated stemness By Jamshed Arslan, Pharm D, PhDBreast cancer is the most common cancer among women that causes the greatest number of cancer-related deaths worldwide. After radiotherapy or cytotoxic chemotherapy like paclitax... Read full blog post. |
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")