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![Western Blot: Spt6 Antibody [NB100-2582] - siRNA knockdown to confirm the specificity of Spt6 antibody (NB100-2582) in C2C12 myoblasts, WB: 1:2000 dilution. 20 ug whole cell lysate. WB image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Spt6-Antibody-Western-Blot-NB100-2582-img0011.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Spt6 Antibody [NB100-2582] - siRNA knockdown to confirm the specificity of Spt6 antibody (NB100-2582) in C2C12 myoblasts, WB: 1:2000 dilution. 20 ug whole cell lysate. WB image submitted by a verified customer review.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry-Paraffin: Spt6 Antibody [NB100-2582] - Sections of human breast carcinoma (left) and mouse squamous cell carcinoma (right). Antibody: Affinity purified rabbit anti- SUPT6H used at a dilution of 1:1,000 (0.2 ug/mL). Detection: DAB](http://images.novusbio.com/fullsize/Spt6-Antibody-Immunohistochemistry-Paraffin-NB100-2582-img0010.jpg)
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: Spt6 Antibody [NB100-2582] - Detection of Human Spt6 on HeLa whole cell lysate using NB100-2582. Spt6 was also immunoprecipitated by another rabbit anti-Spt6 antibody.](http://images.novusbio.com/fullsize/Spt6-Antibody-Western-Blot-NB100-2582-img0007.jpg)
![Western Blot: Spt6 Antibody [NB100-2582] - SPT6 Depletion Induces lncRNA Transcription(A) YWHAZ locus showing pre-mRNA (– strand) & PROMPT (+ strand). SPT6 depletion induced lncRNA as shown by ChRNA-seq & mNET-seq analyses. The profile of mNuc-seq/H3K36me3 is shown below.(B) Meta-analysis of strand-specific ChrRNA-seq signals from −3 kb of TSS to +3 kb of transcription end site (TES) for divergent (pre-mRNA-PROMPT) genes.(C) Boxplots of PROMPT mNET-seq/total CTD & T4P signals.(D) Enhancer located 90 kb downstream of NR4A1 gene with neighboring gene ATG101 showing SPT6 depletion-induced eRNA by ChRNA-seq & mNET-seq. mNuc-seq/H3K4me1 & H3K4me3 signals indicate active enhancer & promoter, respectively.(E) Meta-analysis of eRNA from ChrRNA-seq & mNET-seq (Total) −3 kb to +3 kb from TSS (enhancers with highest eRNA levels selected).(F) Boxplots of eRNA mNET-seq/total CTD & T4P signals at TSS (–/+2 kb).(G & H) Western blot (G) & quantitative RT-PCR (H) of chromatin-bound RNA of parental & SETD2 CRISPR KO U2OS cells with indicated siRNA transfection for 48 hr. Data are represented as mean ± SEM.See also Figure S3. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30449723), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-2582_rabbit-polyclonal-spt6-antibody-31020241552393.jpg)
![Western Blot: Spt6 Antibody [NB100-2582] - SPT6 Depletion Causes H3K36me3 Redistribution(A) Western blots with indicated antibodies 60 hr post-siSPT6 transfection (versus siLuc nonspecific control). EXOSC3 & H3 profiles shown as loading controls.(B) Meta-analysis of reads density (FPKM) for ratio of mNuc-seq/H3K36me3 with H3 at TSS of divergent PROMPT-pre-mRNA & enhancer RNA (eRNA) following SPT6 depletion. All subsequent transcription images employing siSPT6 versus siLuc are shown in red & blue, respectively.(C) Scatterplots of H3K36me3/H3 on pre-mRNA (0 to +3 kb), PROMPT (−3 kb to 0), & eRNA (−3 kb to +3 kb) regions in siLuc versus siSPT6. The percentage of upregulated regions by SPT6 depletion are indicated.(D) Boxplots of mNuc-seq/H3K36me3 ratio across pre-mRNA gene bodies, PROMPTs (3 kb from TSS), & eRNA (2 kb from center) & across lincRNA gene bodies.(E) Model of redistributed H3K36me3 marks caused by SPT6 depletion.See also Figure S2. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30449723), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-2582_rabbit-polyclonal-spt6-antibody-310202415532091.jpg)
![Western Blot: Spt6 Antibody [NB100-2583] - Detection of Human SUPT6H by Western Blot. Samples: Whole cell lysate (5, 15 and 50 ug) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SUPT6H antibody NB100-2583 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/Spt6-Antibody-Western-Blot-NB100-2583-img0007.jpg "Western Blot: Spt6 Antibody [NB100-2583] - Detection of Human SUPT6H by Western Blot. Samples: Whole cell lysate (5, 15 and 50 ug) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SUPT6H antibody NB100-2583 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![Immunohistochemistry: Spt6 Antibody [NB100-2583] - Samples: FFPE serial sections of human ovarian carcinoma Antibody: Affinity purified rabbit anti- SUPT6H A 300-802A-1 used at a dilution of 0.4ug/ml (left) and used at a dilution of 0.2ug/ml (right). Detection: DAB](http://images.novusbio.com/fullsize/Spt6-Antibody-Immunohistochemistry-NB100-2583-img0006.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: Spt6 Antibody [NB100-2583] - Detection of Human SUPT6H on HeLa whole cell lysate using NB100-2583. SUPT6H was successfully immunoprecipitated by another rabbit anti-SUPT6H antibody and NB100-2584.](http://images.novusbio.com/fullsize/Spt6-Antibody-Western-Blot-NB100-2583-img0001.jpg)
![Immunohistochemistry-Paraffin: Spt6 Antibody [NB100-2583] - Sections of human breast carcinoma (left) and mouse squamous cell carcinoma (right).](http://images.novusbio.com/fullsize/Spt6-Antibody-Immunohistochemistry-Paraffin-NB100-2583-img0003.jpg)
![Immunohistochemistry: Spt6 Antibody [NB100-2583] - Samples: FFPE serial sections of human ovarian carcinoma Antibody: Affinity purified rabbit anti- SUPT6H A 300-802A-1 used at a dilution of 0.4ug/ml (left) and used at a dilution of 0.2ug/ml (right) . Detection: DAB](http://images.novusbio.com/fullsize/Spt6-Antibody-Immunohistochemistry-NB100-2583-img0004.jpg)
![Immunohistochemistry: Spt6 Antibody [NB100-2583] - Samples: FFPE serial sections of mouse teratoma Antibody: Affinity purified rabbit anti- SUPT6H A 300-802A-1 used at a dilution of 0.4ug/ml (left) and used at a dilution of 0.2ug/ml (right) . Detection: DAB](http://images.novusbio.com/fullsize/Spt6-Antibody-Immunohistochemistry-NB100-2583-img0005.jpg)
![Western Blot: Spt6 Antibody [NBP2-93722] - Analysis of extracts of various cell lines, using Spt6 at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 10s.](http://images.novusbio.com/fullsize/Spt6-Antibody-Western-Blot-NBP2-93722-img0002.jpg "Western Blot: Spt6 Antibody [NBP2-93722] - Analysis of extracts of various cell lines, using Spt6 at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 10s.")
![Immunocytochemistry/Immunofluorescence: Spt6 Antibody [NBP2-93722] - Analysis of U-2 OS cells using Spt6 at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Spt6-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-93722-img0001.jpg)
![Immunoprecipitation: Spt6 Antibody [NBP2-93722] - Immunoprecipitation analysis of 600ug extracts of Mouse heart using 3ug Spt6 antibody (NBP2-93722). Western blot was performed from the immunoprecipitate using Spt6 antibody (NBP2-93722) at a dilution of 1:500.](http://images.novusbio.com/fullsize/Spt6-Antibody-Immunoprecipitation-NBP2-93722-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Spt6 Antibody [NBP2-34030] - Staining of human cell line U-2 OS shows localization to nucleoplasm, plasma membrane & cytosol.](http://images.novusbio.com/fullsize/Spt6-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-34030-img0003.jpg "Immunocytochemistry/Immunofluorescence: Spt6 Antibody [NBP2-34030] - Staining of human cell line U-2 OS shows localization to nucleoplasm, plasma membrane & cytosol.")
![Immunohistochemistry: Spt6 Antibody [NBP2-34030] - Staining of human breast shows strong nuclear positivity in glandular cells and myoepithelial cells.](http://images.novusbio.com/fullsize/Spt6-Antibody-Immunohistochemistry-NBP2-34030-img0002.jpg)
![Immunoprecipitation: Spt6 Antibody [NB100-2584] - Detection of Human SUPT6H by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-SUPT6H antibodies NB100-2583, NB100-2584 and another SUPT6H Ab used for IP at 3 mcg/mg lysate (A and B). Detection: Chemiluminescence with exposure times of 30 seconds (A and B). * - The asterisk marks a protein with which BL3468 interacts in IP and WB. This band is likely to be a cross-reacting protein.](http://images.novusbio.com/fullsize/Spt6-Antibody-Immunoprecipitation-NB100-2584-img0002.jpg "<b>Independent Antibodies Validation. </b>Immunoprecipitation: Spt6 Antibody [NB100-2584] - Detection of Human SUPT6H by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-SUPT6H antibodies NB100-2583, NB100-2584 and another SUPT6H Ab used for IP at 3 mcg/mg lysate (A and B). Detection: Chemiluminescence with exposure times of 30 seconds (A and B). * - The asterisk marks a protein with which BL3468 interacts in IP and WB. This band is likely to be a cross-reacting protein.")
![Immunohistochemistry: Spt6 Antibody - BSA Free [NBP3-47449] - Immunohistochemistry of paraffin-embedded human kidney using (Spt6 antibody) at dilution of 1:100](http://images.novusbio.com/fullsize/nbp3-47449_rabbit-spt6-pab-bsa-free-28420251372018.jpg "Immunohistochemistry: Spt6 Antibody - BSA Free [NBP3-47449] - Immunohistochemistry of paraffin-embedded human kidney using (Spt6 antibody) at dilution of 1:100")