Spt6 Antibody

Images

 
Genetic Strategies: Western Blot: Spt6 Antibody [NB100-2582] - siRNA knockdown to confirm the specificity of Spt6 antibody (NB100-2582) in C2C12 myoblasts, WB: 1:2000 dilution. 20ug whole cell lysate. Image from ...read more
Immunohistochemistry: Spt6 Antibody [NB100-2582] - Sample: FFPE sections of human breast carcinoma (left) and mouse squamous cell carcinoma (right). Antibody: Affinity purified rabbit anti- SUPT6H used at a dilution of ...read more
Independent Antibodies: Western Blot: Spt6 Antibody [NB100-2582] - Detection of Human Spt6 on HeLa whole cell lysate using NB100-2582. Spt6 was also immunoprecipitated by another rabbit anti-Spt6 antibody.

Product Details

Summary
Reactivity Hu, Mu, ChSpecies Glossary
Applications WB, ChIP, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

Spt6 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 425 and 475 of human Suppressor of Ty 6 Homolog using the numbering given in entry NP_003161.2 (GeneID 6830).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SUPT6H
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
  • Chromatin Immunoprecipitation 1:10-1:500
  • Immunohistochemistry 1:200 - 1:1000
  • Immunohistochemistry-Paraffin 1:200 - 1:1000
  • Immunoprecipitation 2-5 ug/mg lysate
Application Notes
Chip reactivity reported in scientific literature (PMID:23503590)
Positive Control
HeLa Lysate (NB800-PC1)
Reviewed Applications
Read 1 Review rated 5
using
NB100-2582 in the following applications:

Publications
Read Publications using
NB100-2582 in the following applications:

  • 1 publication
  • IP
    1 publication
  • WB
    2 publications

Reactivity Notes

Chicken reactivity reported in scientific literature (PMID: 23008333).

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Spt6 Antibody

  • emb-5
  • hSPT6
  • KIAA0162MGC87943
  • SPT6HSPT6
  • suppressor of Ty (S.cerevisiae) 6 homolog
  • suppressor of Ty 6 homolog (S. cerevisiae)
  • Tat-cotransactivator 2 protein
  • Tat-CT2 protein
  • transcription elongation factor SPT6

Background

Spt6 is a histone H3-H4 chaperone protein that mediates chromatin reassembly behind RNA polymerase II. Spt6 binds both histone H3 and H4 and deposits them on to DNA, which leads to transcriptional repression of target genes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Spt6 Antibody (NB100-2582)(5)

Review for Spt6 Antibody (NB100-2582) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NB100-2582:
Filter by Applications
WB
(1)
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Mouse
(1)
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Images Ratings Applications Species Date Details
Western Blot Spt6 NB100-2582
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reviewed by:
Anonymous
WB Mouse 04/17/2018
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Summary

ApplicationWestern Blot
Sample TestedMouse myoblasts (C2C12 cell lysate),20 ug whole cell lysate
SpeciesMouse
LotA1

Product General Protocols

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Video Protocols

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ChIP Webinar

FAQs for Spt6 Antibody (NB100-2582) (0)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional Array Products

Bioinformatics Tool for Spt6 Antibody (NB100-2582)

Discover related pathways, diseases and genes to Spt6 Antibody (NB100-2582). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for Spt6 Antibody (NB100-2582)

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Pathways for Spt6 Antibody (NB100-2582)

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Blogs on Spt6

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Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Anonymous
04/17/2018
Application: WB
Species: Mouse

Bioinformatics

Gene Symbol SUPT6H
Entrez
Uniprot