Spt6 Antibody

Western Blot: Spt6 Antibody [NB100-2582] - siRNA knockdown to confirm the specificity of Spt6 antibody (NB100-2582) in C2C12 myoblasts, WB: 1:2000 dilution. 20 ug whole cell lysate. WB image submitted by a verified customer review.

Immunohistochemistry-Paraffin: Spt6 Antibody [NB100-2582] - Sections of human breast carcinoma (left) and mouse squamous cell carcinoma (right). Antibody: Affinity purified rabbit anti- SUPT6H used at a dilution of 1:1,000 (0.2 ug/mL). Detection: DAB

Western Blot: Spt6 Antibody [NB100-2582] - Detection of Human Spt6 on HeLa whole cell lysate using NB100-2582. Spt6 was also immunoprecipitated by another rabbit anti-Spt6 antibody.

• Reactivity: Hu, Mu, Ch
• Applications: WB, ChIP, IHC, IHC-P, IP, KD
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

Spt6 Antibody Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residue 425 and 475 of human Suppressor of Ty 6 Homolog using the numbering given in entry NP_003161.2 (GeneID 6830).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SUPT6H
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation 1:10-1:500
  • Immunohistochemistry 1:200-1:1000
  • Immunohistochemistry-Paraffin 1:200-1:1000
  • Immunoprecipitation 2-5 ug/mg lysate
  • Knockdown Validated
  • Western Blot 1:2000-1:10000
• Application Notes: Chip reactivity reported in scientific literature (PMID:23503590). Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. Use in WB reported in scientific publication (PMID: 32427586). Spt6 antibody validated for WB from a verified customer review.
• Control:

  HeLa Whole Cell Lysate

Reactivity Notes

Chicken reactivity reported in scientific literature (PMID: 23008333).

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS, 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Spt6 Antibody

• emb-5
• hSPT6
• KIAA0162MGC87943
• SPT6HSPT6
• suppressor of Ty (S.cerevisiae) 6 homolog
• suppressor of Ty 6 homolog (S. cerevisiae)
• Tat-cotransactivator 2 protein
• Tat-CT2 protein
• transcription elongation factor SPT6

Background

Spt6 is a histone H3-H4 chaperone protein that mediates chromatin reassembly behind RNA polymerase II. Spt6 binds both histone H3 and H4 and deposits them on to DNA, which leads to transcriptional repression of target genes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Spt6 Antibody (NB100-2582)(16)

Publications using NB100-2582

• Studniarek C, Tellier M, Martin PGP et al. The 7SK/P-TEFb snRNP controls ultraviolet radiation-induced transcriptional reprogramming Cell reports 2021-04-13 [PMID: 33852864]
• Lu H, Xie Y, Tran L et al. Chemotherapy-induced S100A10 recruits KDM6A to facilitate OCT4-mediated breast cancer stemness J Clin Invest. 2020-05-19 [PMID: 32427586] (IP, KD, WB, Human)
• Chirackal Manavalan AP, Pilarova K, Kluge M et al. CDK12 controls G1/S progression by regulating RNAPII processivity at core DNA replication genes EMBO Rep. 2019-07-25 [PMID: 31347271] (IP, Human)
• Baluapuri A, Hofstetter J, Dudvarski Stankovic N et al. MYC Recruits SPT5 to RNA Polymerase II to Promote Processive Transcription Elongation Mol. Cell 2019-03-21 [PMID: 30928206] (WB, Mouse)
• Nojima T, Tellier M, Foxwell J et al. Deregulated Expression of Mammalian lncRNA through Loss of SPT6 Induces R-Loop Formation, Replication Stress, and Cellular Senescence. Toxicol. Sci. 2018-11-21 [PMID: 30449723] (Human)
• Nojima T, Rebelo k, Gomes T et al. RNA Polymerase II Phosphorylated on CTD Serine 5 Interacts with the Spliceosome during Co-transcriptional Splicing. Mol. Cell. 2018-10-18 [PMID: 30340024] (Human)
• Wang AH, Juan AH, Ko KD et al. The Elongation Factor Spt6 Maintains ESC Pluripotency by Controlling Super-Enhancers and Counteracting Polycomb Proteins. Mol. Cell. 2017-10-19 [PMID: 29033324] (WB)
• Wang AH, Zare H, Mousavi K et al. The histone chaperone Spt6 coordinates histone H3K27 demethylation and myogenesis. EMBO J 2013-03-15 [PMID: 23503590] (ChIP, WB, Mouse)
• Willmann KL, Milosevic S, Pauklin S et al. A role for the RNA pol II-associated PAF complex in AID-induced immune diversification. J Exp Med. 2012-10-22 [PMID: 23008333] (IP, Chicken, Mouse)
• Chen S, Ma J, Wu F et al. The histone H3 Lys 27 demethylase JMJD3 regulates gene expression by impacting transcriptional elongation. Genes Dev 2012-06-01 [PMID: 22713873]
• Kronemann D, Hagemeier SR, Cygnar D et al. Binding of the human cytomegalovirus (HCMV) tegument protein UL69 to UAP56/URH49 is not required for efficient replication of HCMV. J Virol 2010-09-01 [PMID: 20610707]
• Liu X, Guo Z, Han J et al. The PAF1 complex promotes 3' processing of pervasive transcripts Cell reports 2022-03-15 [PMID: 35294889] (WB, Mouse)
• Narain A, Bhandare P, Adhikari B Et Al. Targeted protein degradation reveals a direct role of SPT6 in RNAPII elongation and termination Molecular cell 2021-06-30 [PMID: 34233157] (ChIP)
• Obara E. A. A, Aguilar-Morante D, et al. SPT6-driven error-free DNA repair safeguards genomic stability of glioblastoma cancer stem-like cells. Nat Commun 2020-09-18 [PMID: 32948765] (ChIP, Human)
• Hofstetter JEI MYC shapes the composition of RNA polymerase II through direct recruitment of transcription elongation factors Thesis 2022-01-01

Reviews for Spt6 Antibody (NB100-2582) (2)

Average Rating: 5

(Based on 2 reviews)

We have 2 reviews tested in 2 species: Human, Mouse.

reviewed by: Verified Customer WB Human 03/06/2021

Summary

• Application: Western Blot
• Sample Tested: breast cancer cell line, Sample Amount: 40
• Species: Human
• Lot: A2

reviewed by: Liming Hou WB Mouse 04/17/2018

Summary

• Application: Western Blot
• Sample Tested: 20 ug whole cell lysate,Mouse myoblasts (C2C12 cell lysate)
• Species: Mouse
• Lot: A1

Bioinformatics

• Gene Symbol: SUPT6H
• Entrez:
  • 6830(Human)
• Uniprot:
  • Q7KZ85(Human)