Genetic Strategies: Western Blot: Spt6 Antibody [NB100-2582] - siRNA knockdown to confirm the specificity of Spt6 antibody (NB100-2582) in C2C12 myoblasts, WB: 1:2000 dilution. 20 ug whole cell lysate. WB image ...read more
Immunohistochemistry-Paraffin: Spt6 Antibody [NB100-2582] - Sections of human breast carcinoma (left) and mouse squamous cell carcinoma (right). Antibody: Affinity purified rabbit anti- SUPT6H used at a dilution of ...read more
Independent Antibodies: Western Blot: Spt6 Antibody [NB100-2582] - Detection of Human Spt6 on HeLa whole cell lysate using NB100-2582. Spt6 was also immunoprecipitated by another rabbit anti-Spt6 antibody.
Chip reactivity reported in scientific literature (PMID:23503590). Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. Use in WB reported in scientific publication (PMID: 32427586). Spt6 antibody validated for WB from a verified customer review.
Chicken reactivity reported in scientific literature (PMID: 23008333).
Packaging, Storage & Formulations
Store at 4C. Do not freeze.
TBS, 0.1% BSA
0.09% Sodium Azide
Immunogen affinity purified
Alternate Names for Spt6 Antibody
suppressor of Ty (S.cerevisiae) 6 homolog
suppressor of Ty 6 homolog (S. cerevisiae)
Tat-cotransactivator 2 protein
transcription elongation factor SPT6
Spt6 is a histone H3-H4 chaperone protein that mediates chromatin reassembly behind RNA polymerase II. Spt6 binds both histone H3 and H4 and deposits them on to DNA, which leads to transcriptional repression of target genes.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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