Spt6 Antibody

Images

 
Genetic Strategies: Western Blot: Spt6 Antibody [NB100-2582] - siRNA knockdown to confirm the specificity of Spt6 antibody (NB100-2582) in C2C12 myoblasts, WB: 1:2000 dilution. 20ug whole cell lysate. Image from ...read more
Immunohistochemistry-Paraffin: Spt6 Antibody [NB100-2582] - Sections of human breast carcinoma (left) and mouse squamous cell carcinoma (right). Antibody: Affinity purified rabbit anti- SUPT6H used at a dilution of ...read more
Independent Antibodies: Western Blot: Spt6 Antibody [NB100-2582] - Detection of Human Spt6 on HeLa whole cell lysate using NB100-2582. Spt6 was also immunoprecipitated by another rabbit anti-Spt6 antibody.

Product Details

Summary
Reactivity Hu, Mu, ChSpecies Glossary
Applications WB, ChIP, IHC, IHC-P, IP, KD
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

Spt6 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 425 and 475 of human Suppressor of Ty 6 Homolog using the numbering given in entry NP_003161.2 (GeneID 6830).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SUPT6H
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
  • Chromatin Immunoprecipitation 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:100-1:500
  • Immunoprecipitation 2-5 ug/mg lysate
  • Knockdown Validated
Application Notes
Chip reactivity reported in scientific literature (PMID:23503590). Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Control
HeLa Whole Cell Lysate
Reviewed Applications
Read 1 Review rated 5
using
NB100-2582 in the following applications:

Publications
Read Publications using
NB100-2582 in the following applications:

  • 1 publication
  • IP
    2 publications
  • WB
    3 publications

Reactivity Notes

Chicken reactivity reported in scientific literature (PMID: 23008333).

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Spt6 Antibody

  • emb-5
  • hSPT6
  • KIAA0162MGC87943
  • SPT6HSPT6
  • suppressor of Ty (S.cerevisiae) 6 homolog
  • suppressor of Ty 6 homolog (S. cerevisiae)
  • Tat-cotransactivator 2 protein
  • Tat-CT2 protein
  • transcription elongation factor SPT6

Background

Spt6 is a histone H3-H4 chaperone protein that mediates chromatin reassembly behind RNA polymerase II. Spt6 binds both histone H3 and H4 and deposits them on to DNA, which leads to transcriptional repression of target genes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Spt6 Antibody (NB100-2582)(9)

We have publications tested in 3 confirmed species: Human, Mouse, Chicken.

We have publications tested in 3 applications: ChIP, IP, WB.


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(1)
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(2)
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(3)
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Showing Publications 1 - 9 of 9.
Publications using NB100-2582 Applications Species
Chirackal Manavalan AP, Pilarova K, Kluge M et al. CDK12 controls G1/S progression by regulating RNAPII processivity at core DNA replication genes EMBO Rep. Jul 25 2019 [PMID: 31347271] (IP, Human) IP Human
Baluapuri A, Hofstetter J, Dudvarski Stankovic N et al. MYC Recruits SPT5 to RNA Polymerase II to Promote Processive Transcription Elongation Mol. Cell Mar 21 2019 [PMID: 30928206] (WB, Mouse) WB Mouse
Nojima T, Tellier M, Foxwell J et al. Deregulated Expression of Mammalian lncRNA through Loss of SPT6 Induces R-Loop Formation, Replication Stress, and Cellular Senescence. Toxicol. Sci. Nov 21 2018 [PMID: 30449723] (Human) Human
Nojima T, Rebelo k, Gomes T et al. RNA Polymerase II Phosphorylated on CTD Serine 5 Interacts with the Spliceosome during Co-transcriptional Splicing. Mol. Cell. Oct 18 2018 [PMID: 30340024] (Human) Human
Wang AH, Juan AH, Ko KD et al. The Elongation Factor Spt6 Maintains ESC Pluripotency by Controlling Super-Enhancers and Counteracting Polycomb Proteins. Mol. Cell. 2017 Oct 19 [PMID: 29033324] (WB) WB
Wang AH, Zare H, Mousavi K et al. The histone chaperone Spt6 coordinates histone H3K27 demethylation and myogenesis. EMBO J 2013 Mar 15 [PMID: 23503590] (WB, ChIP, Mouse) WB, ChIP Mouse
Willmann KL, Milosevic S, Pauklin S et al. A role for the RNA pol II-associated PAF complex in AID-induced immune diversification. J Exp Med. 2012 Oct 22 [PMID: 23008333] (IP, Mouse, Chicken) IP Mouse, Chicken
Chen S, Ma J, Wu F et al. The histone H3 Lys 27 demethylase JMJD3 regulates gene expression by impacting transcriptional elongation. Genes Dev 2012 Jun [PMID: 22713873]
Kronemann D, Hagemeier SR, Cygnar D et al. Binding of the human cytomegalovirus (HCMV) tegument protein UL69 to UAP56/URH49 is not required for efficient replication of HCMV. J Virol 2010 Sep [PMID: 20610707]

Review for Spt6 Antibody (NB100-2582) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NB100-2582:
Filter by Applications
WB
(1)
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Filter by Species
Mouse
(1)
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Images Ratings Applications Species Date Details
Western Blot Spt6 NB100-2582
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reviewed by:
Anonymous
WB Mouse 04/17/2018
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Summary

ApplicationWestern Blot
Sample TestedMouse myoblasts (C2C12 cell lysate),20 ug whole cell lysate
SpeciesMouse
LotA1

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Control Lysate(s)

Secondary Antibodies

 

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Recent Reviews

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5
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1
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Anonymous
04/17/2018
Application: WB
Species: Mouse

Bioinformatics

Gene Symbol SUPT6H
Entrez
Uniprot
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