Sphingosine Kinase 1/SPHK1 Antibody [Alexa Fluor® 405]

• Reactivity: Hu
• Applications: WB, ELISA, IHC, IP, ICC/IF
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor 405

Sphingosine Kinase 1/SPHK1 Antibody [Alexa Fluor® 405] Summary

• Immunogen: S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Sphingosine Kinase 1/SPHK1
  Asp2-Leu398
  Accession # NP_068807
• Specificity: Detects human SPHK1 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human SPHK2 is observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry
  • Immunohistochemistry
  • Immunoprecipitation
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Sphingosine Kinase 1/SPHK1 Antibody [Alexa Fluor® 405]

• EC 2.7.1.91
• SK1
• sphingosine kinase 1
• SPHK1
• SPHKSK 1
• SPK 1
• SPK
• SPK1

Background

Sphingosine kinases are cytosolic or membrane-associated enzymes that catalyze the phosphorylation of sphingosine to sphingosine-1-phosphate (S1P). Two types of sphingosine kinases, SPHK1 and SPHK2, are known to be expressed in human cells. The two enzymes share considerable amino acid sequence similarity, but differ in their N‑terminal and central regions (1). The two proteins also differ in tissue distribution and some kinetic properties (1). S1P is a lipid messenger that regulates diverse physiological processes including cell proliferation, migration, apoptosis, inflammation, calcium homeostasis and cytoskeletal structure (2, 3). The level of S1P is tightly controlled by SPHKs and S1P degrading enzymes. SPHK1 and its activation can be stimulated by several growth factors such as tumor necrosis factor-alpha , epidermal growth factor and transforming growth factor-beta (3, 4). Expression of SPHK1 has been found to increase in many human solid tumors and overexpression of SPHK1 is associated with tumor angiogenesis (5). Such studies have implicated SPHK1 as a new target for cancer treatment.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.