SPG3A Antibody (1B9) - Azide and BSA Free

Western Blot: SPG3A Antibody (1B9) [H00051062-M03] - Analysis of SPG3A expression in PC-12 (Cat # L012V1).

Western Blot: SPG3A Antibody (1B9) [H00051062-M03] - Analysis of SPG3A expression in IMR-32 (Cat # L008V1).

ELISA: SPG3A Antibody (1B9) [H00051062-M03] - Detection limit for recombinant GST tagged SPG3A is approximately 1ng/ml as a capture antibody.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, IHC
• Clone: 1B9
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

SPG3A Antibody (1B9) - Azide and BSA Free Summary

• Immunogen: SPG3A (NP_056999, 1 a.a. ~ 100 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MAKNRRDRNSWGGFSEKTYEWSSEEEEPVKKAGPVQVLIVKDDHSFELDETALNRILLSEAVRDKEVVAVSVAGAFRKGKSFLMDFMLRYMYNQESVDWV
• Specificity: SPG3A - spastic paraplegia 3A (autosomal dominant)
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: ATL1
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500
• Application Notes: Antibody reactive against cell lysate and recombinant protein for western blot. It has also been used for ELISA. Use in Immunohistochemistry-paraffin reported in scientific literature (PMID:31396305).

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 31396305).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for SPG3A Antibody (1B9) - Azide and BSA Free

• AD-FSP
• ATL1
• atlastin GTPase 1
• atlastin1
• atlastin-1
• Brain-specific GTP-binding protein
• EC 3.6.5.-
• FSP1
• GBP3
• GBP-3
• GTP-binding protein 3
• Guanine nucleotide-binding protein 3
• guanylate-binding protein 3
• hGBP3
• Spastic paraplegia 3 protein A
• spastic paraplegia 3A (autosomal dominant)
• SPG3
• SPG3A
• SPG3AhGBP3

Background

The protein encoded by this gene is a GTPase and a Golgi body transmembrane protein. The encoded protein can form a homotetramer and has been shown to interact with spastin and with mitogen-activated protein kinase kinase kinase kinase 4. This protein may be involved in axonal maintenance as evidenced by the fact that defects in this gene are a cause of spastic paraplegia type 3. Three transcript variants encoding two different isoforms have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SPG3A Antibody (H00051062-M03)(1)

Publication using H00051062-M03

• SchUler R, Efentakis P, Wild J et al. T Cell-Derived IL-17A Induces Vascular Dysfunction via Perivascular Fibrosis Formation and Dysregulation of NO/cGMP Signaling Oxidative Medicine and Cellular Longevity 2019-07-18 [PMID: 31396305] (IHC-P, Mouse)

Bioinformatics

• Gene Symbol: ATL1
• Entrez:
  • 51062(Human)
• OMIM:
  • 182600(Human)
• Uniprot:
  • NP_056999(Human)