SPFH2 Antibody (9T3M5)

Western Blot: SPFH2 Antibody (9T3M5) [NBP3-15305] - Western blot analysis of extracts of various cell lines, using SPFH2 antibody (NBP3-15305) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Immunohistochemistry-Paraffin: SPFH2 Antibody (9T3M5) [NBP3-15305] - Immunohistochemistry of paraffin-embedded rat ovary using SPFH2 Rabbit mAb (NBP3-15305) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunohistochemistry-Paraffin: SPFH2 Antibody (9T3M5) [NBP3-15305] - Immunohistochemistry of paraffin-embedded mouse kidney using SPFH2 Rabbit mAb (NBP3-15305) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clone: 9T3M5
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

SPFH2 Antibody (9T3M5) Summary

• Description: Novus Biologicals Rabbit SPFH2 Antibody (9T3M5) (NBP3-15305) is a recombinant monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthetic peptide corresponding to a sequence within amino acids 50-150 of human SPFH2 (O94905). HLMLPFITSYKSVQTTLQTDEVKNVPCGTSGGVMIYFDRIEVVNFLVPNAVYDIVKNYTADYDKALIFNKIHHELNQFCSVHTLQEVYIELFDQIDENLKL
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: ERLIN2
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500 - 1:1000

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol, 0.05% BSA
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for SPFH2 Antibody (9T3M5)

• C8orf2SPFH domain-containing protein 2
• Endoplasmic reticulum lipid raft-associated protein 2
• ER lipid raft associated 2
• Erlin-2
• MGC87072
• NET32
• SPFH domain family, member 2
• SPFH2chromosome 8 open reading frame 2
• Stomatin-prohibitin-flotillin-HflC/K domain-containing protein 2

Background

SPFH2 is a component of the ERLIN1/ERLIN2 complex which mediates the endoplasmic reticulum-associated degradation (ERAD) of inositol 1,4,5-trisphosphate receptors (IP3Rs). Also involved in ITPR1 degradation by the ERAD pathway

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ERLIN2