SPCS1 Antibody - BSA Free

Immunohistochemistry-Paraffin: SPCS1 Antibody [NBP1-92433] - Staining of human lymphoid tissues shows strong cytoplasmic positivity in a subset of non-germinal center cells.

Immunohistochemistry-Paraffin: SPCS1 Antibody [NBP1-92433] - Staining of human stomach, upper shows strong cytoplasmic positivity in glandular cells.

Immunohistochemistry-Paraffin: SPCS1 Antibody [NBP1-92433] - Staining of human kidney shows moderate cytoplasmic positivity in cells in tubules.

Immunohistochemistry-Paraffin: SPCS1 Antibody [NBP1-92433] - Staining of human stomach shows strong cytoplasmic positivity in a subset of glandular cells.

Immunohistochemistry-Paraffin: SPCS1 Antibody [NBP1-92433] - Staining of human prostate shows moderate positivity in glandular cells.

Analysis in control (vector only transfected HEK293T lysate) and SPCS1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells).

• Reactivity: Hu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

SPCS1 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit SPCS1 Antibody - BSA Free (NBP1-92433) is a polyclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: PPWPIYRRHPLKWLPVQESSTDDKKPG
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SPCS1
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:20 - 1:50
  • Immunohistochemistry-Paraffin 1:20 - 1:50
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.
• Control Peptide:

  SPCS1 Protein (NBP1-92433PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (81%), Rat (85%)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for SPCS1 Antibody - BSA Free

• EC 3.4
• HSPC033
• Microsomal signal peptidase 12 kDa subunit
• signal peptidase 12kDa
• signal peptidase complex subunit 1 homolog (S. cerevisiae)
• signal peptidase complex subunit 1
• SPase 12 kDa subunit
• SPC12SPC1
• YJR010C-A

Background

SPCS1 is a component of the microsomal signal peptidase complex which removes signal peptides from nascent proteins as they are translocated into the lumen of the endoplasmic reticulum

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SPCS1