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PC6 Antibody

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Immunocytochemistry/Immunofluorescence: PC6 Antibody [NBP1-87326] - Immunofluorescent staining of human cell line RH-30 shows localization to the Golgi apparatus.
Immunocytochemistry/ Immunofluorescence: PC6 Antibody [NBP1-87326] - Immunofluorescent staining of human cell line RH-30 shows localization to the Golgi apparatus.
Immunohistochemistry-Paraffin: PC6 Antibody [NBP1-87326] - Staining in human duodenum and lymph node tissues using anti-PCSK5 antibody. Corresponding PCSK5 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: PC6 Antibody [NBP1-87326] - Staining of human duodenum shows high expression.
Immunohistochemistry-Paraffin: PC6 Antibody [NBP1-87326] - Staining of human lymph node shows low expression as expected.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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PC6 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: YLEHVVVRITITHPRRGDLAIYLTSPSGTRSQLLANRLFDHSMEGFKNWEFMTIHCWGERAAGDWVLEVYDTPSQLRNFKTPGKLKEWSLVLYGTSVQPYSPTNEFPKVERFRYSRVEDPTDD
Specificity
Specificity of human PC6 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
Predicted Species
Mouse (100%), Rat (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PCSK5
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 1 - 4 ug/ml
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:20 - 1:50
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
PC6 Protein (NBP1-87326PEP)
Publications
Read Publication using NBP1-87326.

Reactivity Notes

Reactivity reported in scientific literature (PMID: 23435261)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for PC6 Antibody

  • EC 3.4.21
  • EC 3.4.21.-
  • EC 3.4.21.61
  • EC 3.4.21.75
  • FLJ11149
  • FLJ16215
  • hPC6
  • PC5PC6A
  • PC6prohormone convertase 5
  • Proprotein convertase 5
  • Proprotein convertase 6
  • proprotein convertase PC5
  • proprotein convertase subtilisin/kexin type 5
  • protease PC6
  • SPC6
  • Subtilisin/kexin-like protease PC5

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB100-1903
Western Blot: Furin Antibody [NB100-1903] - Analysis was performed on membrane enriched extracts (30 ug lysate) of HeLa (Lane 1), HEK 293 (Lane 2), K-562 (Lane 3), SH-SY5Y (Lane 4) and 10uL conditioned media from HeLa cell line (Lane 5).
Immunocytochemistry/Immunofluorescence: Furin Antibody [NB100-1903] - Immunolocalization of endogenous furin in mouse 3T3 cells.
Flow Cytometry: Furin Antibody [NB100-1903] - Flow cytometry analysis of Furin Convertase was done on HeLa cells. Cells were fixed with 70% ethanol for 10 minutes, permeabilized with 0.25% Triton (R) X-100 for 20 minutes, and blocked with 5% BSA for 30 minutes at room temperature. Cells were labeled with Furin Convertase Rabbit Polyclonal Antibody or with rabbit isotype control (pink histogram) at 3-5 ug/million cells in 2.5% BSA. After incubation at room temperature for 2 hours, the cells were labeled with Alexa Fluor (R) 488 Goat Anti-Rabbit Secondary Antibody (A11008) at a dilution of 1:400 for 30 minutes at room temperature. The representative 10, 000 cells were acquired and analyzed for each sample using an Attune (R) Acoustic Focusing Cytometer. The purple histogram represents unstained control cells and the green histogram represents no-primary-antibody control.
(1 Review)
Species: Hu, Mu, Rt, Po, Ca, Ha, Pm
Applications: WB, B/N, Flow, ICC/IF, IHC-P, IP
PC1/3 Antibody
(3D2)
H00005122-M02
Western Blot: PC1/3 Antibody (3D2) [H00005122-M02] - PCSK1 monoclonal antibody (M02), clone 3D2. Analysis of PCSK1 expression in Raw 264.7.
Immunocytochemistry/Immunofluorescence: PC1/3 Antibody (3D2) [H00005122-M02] - Analysis of monoclonal antibody to PCSK1 on HeLa cell. Antibody concentration 30 ug/ml.
Immunohistochemistry-Paraffin: PC1/3 Antibody (3D2) [H00005122-M02] - Analysis of monoclonal antibody to PCSK1 on formalin-fixed paraffin-embedded human small Intestine. Antibody concentration 3 ug/ml.
(1 Publication)
Species: Hu, Mu, Rt
Applications: WB, ELISA, ICC/IF, IHC-P
PACE4 Antibody
H00005046-A01
Western Blot: PACE4 Antibody [H00005046-A01] - Analysis of PCSK6 expression in PC-12.
Western Blot: PACE4 Antibody [H00005046-A01] - Analysis of PCSK6 expression in Hela S3 NE (Cat # L013V3 ).
Western Blot: PACE4 Antibody [H00005046-A01] - Analysis of PCSK6 expression in NIH/3T3.
Species: Hu, Mu, Rt
Applications: WB, ELISA
Proprotein
Convertase 7/PCSK7
2984-SE
Proprotein Convertase 7/PCSK7
Species: Hu
Proprotein
Convertase 2/PCSK2 An
AF6018
Western blot shows lysates of MCF‑7 human breast cancer cell line, beta TC‑6 mouse beta cell insulinoma cell line, and HepG2 human hepatocellular carcinoma cell line. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human Proprotein Convertase 2/PCSK2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6018) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Proprotein Convertase 2/PCSK2 at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Proprotein Convertase 2/PCSK2 was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Sheep Anti-Human/Mouse Proprotein Convertase 2/PCSK2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6018) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue).This application has not been tested in mouse samples. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
(1 Publication)
Species: Hu, Mu
Applications: WB, IP, ICC
Cytokeratin 17
Antibody (E3 (same as ...
NBP2-29421
Western Blot: Cytokeratin 17 Antibody (E3) [NBP2-29421] - Analysis of Cytokeratin 17 in human HeLa lysate using Cytokeratin 17 E3 antibody at 3 ug/ml. goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.
Immunocytochemistry/Immunofluorescence: Cytokeratin 17 Antibody (E3) [NBP2-29421] - Formalin-paraffin skin stained with CK17 Ab (E3).
Immunohistochemistry-Paraffin: Cytokeratin 17 Antibody (E3 (same as Ks17.E3)) [NBP2-29421] - Formalin-fixed paraffin-embedded human Bladder Carcinoma stained with CK17 Monoclonal Antibody (E3)
(1 Publication)
Species: Hu, Rt, Po, Bv, Gt
Applications: WB, ELISA, Flow, ICC/IF, IHC-Fr, IHC-P, IP
CBX4 Antibody
(6C5G3)
NBP2-52432
Western Blot: CBX4 Antibody (6C5G3) [NBP2-52432] - Analysis using CBX4 mAb against human CBX4 (AA: 397-514) recombinant protein. (Expected MW is 43.3 kDa)
Immunohistochemistry: CBX4 Antibody (6C5G3) [NBP2-52432] - Analysis of paraffin-embedded rectum cancer tissues using CBX4 mouse mAb with DAB staining.
Flow Cytometry: CBX4 Antibody (6C5G3) [NBP2-52432] - Flow cytometric analysis of Hela cells using CBX4 mouse mAb (green) and negative control (red).
Species: Hu
Applications: WB, ELISA, Flow, IHC, CyTOF-ready, ICC
Polycystin 2
Antibody
NBP1-80944
Immunocytochemistry/Immunofluorescence: Polycystin 2 Antibody [NBP1-80944] - Staining of human cell line BJ shows localization to endoplasmic reticulum. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Polycystin 2 Antibody [NBP1-80944] - Staining of human kidney shows positivity in a subset of renal tubules.
Species: Hu
Applications: ICC/IF, IHC, IHC-P
Cytokeratin 6b
Antibody
NBP2-14924
Western Blot: Cytokeratin 6b Antibody [NBP2-14924] - Sample (1 ug of whole cell lysate) A: HeLa 7. 5% SDS PAGE; antibody diluted at 1:5000.
Immunocytochemistry/Immunofluorescence: Cytokeratin 6b Antibody [NBP2-14924] - Analysis of methanol-fixed HeLa, using antibody at 1:500 dilution.
Immunohistochemistry-Paraffin: Cytokeratin 6b Antibody [NBP2-14924] - Paraffin-embedded mouse brain, using antibody at 1:500 dilution.
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
ENPP-1 Antibody
NBP2-27561
Western Blot: ENPP-1 Antibody [NBP2-27561] - HEK293 overexpressing Human ENPP1 and probed with mock transfection in first lane.
Immunohistochemistry-Paraffin: ENPP-1 Antibody [NBP2-27561] - Staining of paraffin embedded Human Liver. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.
(6 Publications)
Species: Hu
Applications: WB, IHC, IHC-P, PEP-ELISA
Proprotein
convertase PC4 Antibo
NBP1-88010
Immunohistochemistry-Paraffin: Proprotein convertase PC4 Antibody [NBP1-88010] - Staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts and Leydig cells.
Immunohistochemistry: Proprotein convertase PC4 Antibody [NBP1-88010] - Staining of human cerebral cortex shows strong cytoplasmic positivity in granular pattern in neuronal cells.
Species: Hu
Applications: IHC, IHC-P
SUB1 Antibody
NBP1-82454
Western Blot: SUB1 Antibody [NBP1-82454] - Western blot analysis in mouse cell line NIH-3T3, rat cell line NBT-II and rat cell line pC12.
Immunocytochemistry/Immunofluorescence: SUB1 Antibody [NBP1-82454] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleus & nucleoli.
Immunohistochemistry-Paraffin: SUB1 Antibody [NBP1-82454] - Staining of human breast shows strong nuclear positivity in glandular cells.
(2 Publications)
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
IFRD1 Antibody
NBP2-24679
Western Blot: IFRD1 Antibody [NBP2-24679] - Analysis of Tis7 in HeLa cell lysate in the 1) absence 2) presence of immunizing peptide and 3) RAW cell lysate using this antibody. 0 ug/ml, 1.0 ug/ml and 4.0 ug/ml respectively.
Immunohistochemistry-Paraffin: IFRD1 Antibody [NBP2-24679] - Immunohistochemical analysis of IFRD1 in formalin-fixed, paraffin-embedded humanrectum tissue using an isotype control (top) and NBP2-24679 (bottom) at 5 ug/ml.
Species: Hu, Po, Bv, Ca, Pm
Applications: WB, IHC-P
Cbx8 Antibody
NBP1-83224
Western Blot: Cbx8 Antibody [NBP1-83224] - Analysis in human cell line A-549.
Immunocytochemistry/Immunofluorescence: Cbx8 Antibody [NBP1-83224] - Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.
Immunohistochemistry-Paraffin: Cbx8 Antibody [NBP1-83224] - Staining of human pancreas shows low expression as expected.
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
ACTR1B Antibody
NBP2-15275
Western Blot: ACTR1B Antibody [NBP2-15275] - Sample (30 ug of whole cell lysate) A: IMR32 10% SDS PAGE; antibody diluted at 1:1000.
Immunocytochemistry/Immunofluorescence: ACTR1B Antibody [NBP2-15275] - SKNSH cells were fixed in ice-cold MeOH for 5 min. Green: ACTR1B protein stained by ACTR1B antibody diluted at 1:1000. Blue: Hoechst 33342 staining.
Immunohistochemistry-Paraffin: ACTR1B Antibody [NBP2-15275] - Paraffin-embedded ovarian carcinoma. ACTR1B antibody dilution: 1:500.
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
BTG2 Antibody
NBP1-81215
Western Blot: BTG2 Antibody [NBP1-81215] - Analysis in control (vector only transfected HEK293T lysate) and bTG2 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/Immunofluorescence: BTG2 Antibody [NBP1-81215] - Immunofluorescent staining of human cell line A-431 shows localization to vesicles.
Immunohistochemistry-Paraffin: BTG2 Antibody [NBP1-81215] - Staining of human cerebellum shows strong cytoplasmic positivity in purkinje cells.
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
Recombinant Human
MBTPS1 Protein
H00008720-Q01
12.5% SDS-PAGE Stained with Coomassie Blue.
Species: Hu
Proprotein
Convertase 9/PCSK9 An
NBP2-31364
Western Blot: PCSK9 Antibody [NBP2-31364] - Western blot analysis of human lung tissue (A) using PCSK9 antibody at a concentration of 2 ug/ml.
Immunocytochemistry/Immunofluorescence: PCSK9 Antibody [NBP2-31364] - PCSK9 antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red). A concentration of 0.1 ug/ml was used. Image objective 40x.
Immunohistochemistry-Paraffin: PCSK9 Antibody [NBP2-31364] - IHC-P analysis of PCSK9 protein in a section of normal skin from human using 5 ug/ml concentration of PCSK9 antibody. The keratinocytes showed an expected cytoplasmic staining pattern with more intensity towards the cellular periphery, whereas the pigmented basel cell layer depicted relatively milder staining.
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P

Publications for PC6 Antibody (NBP1-87326)(1)


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Bioinformatics

Gene Symbol PCSK5