![Immunocytochemistry/Immunofluorescence: PC6 Antibody [NBP1-87326] - Immunofluorescent staining of human cell line RH-30 shows localization to the Golgi apparatus.](http://images.novusbio.com/fullsize2/PC6-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87326-img0007.jpg "Immunocytochemistry/Immunofluorescence: PC6 Antibody [NBP1-87326] - Immunofluorescent staining of human cell line RH-30 shows localization to the Golgi apparatus.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | ICC/IF, IHC, IHC-P |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | This antibody was developed against Recombinant Protein corresponding to amino acids: YLEHVVVRITITHPRRGDLAIYLTSPSGTRSQLLANRLFDHSMEGFKNWEFMTIHCWGERAAGDWVLEVYDTPSQLRNFKTPGKLKEWSLVLYGTSVQPYSPTNEFPKVERFRYSRVEDPTDD |
| Specificity | Specificity of human PC6 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins. |
| Predicted Species | Mouse (100%), Rat (100%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | PCSK5 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100. |
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| Publications |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.2) and 40% Glycerol |
| Preservative | 0.02% Sodium Azide |
| Purity | Immunogen affinity purified |
| Publication using NBP1-87326 | Applications | Species |
|---|---|---|
| Stadler C, Rexhepaj E, Singan VR et al. Immunofluorescence and fluorescent-protein tagging show high correlation for protein localization in mammalian cells. Nat Methods 2013 Apr [PMID: 23435261] |
Secondary Antibodies |
Isotype Controls |
Diseases for PC6 Antibody (NBP1-87326)Discover more about diseases related to PC6 Antibody (NBP1-87326).
| Pathways for PC6 Antibody (NBP1-87326)View related products by pathway.
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PTMs for PC6 Antibody (NBP1-87326)Learn more about PTMs related to PC6 Antibody (NBP1-87326).
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| Gene Symbol | PCSK5 |
![Immunohistochemistry-Paraffin: PC6 Antibody [NBP1-87326] - Staining in human duodenum and lymph node tissues using anti-PCSK5 antibody. Corresponding PCSK5 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize2/PC6-Antibody-Immunohistochemistry-Paraffin-NBP1-87326-img0010.jpg "Immunohistochemistry-Paraffin: PC6 Antibody [NBP1-87326] - Staining in human duodenum and lymph node tissues using anti-PCSK5 antibody. Corresponding PCSK5 RNA-seq data are presented for the same tissues.")
![Immunohistochemistry-Paraffin: PC6 Antibody [NBP1-87326] - Staining of human duodenum shows high expression.](http://images.novusbio.com/fullsize2/PC6-Antibody-Immunohistochemistry-Paraffin-NBP1-87326-img0008.jpg "Immunohistochemistry-Paraffin: PC6 Antibody [NBP1-87326] - Staining of human duodenum shows high expression.")
![Immunohistochemistry-Paraffin: PC6 Antibody [NBP1-87326] - Staining of human lymph node shows low expression as expected.](http://images.novusbio.com/fullsize2/PC6-Antibody-Immunohistochemistry-Paraffin-NBP1-87326-img0009.jpg "Immunohistochemistry-Paraffin: PC6 Antibody [NBP1-87326] - Staining of human lymph node shows low expression as expected.")
![Western Blot: Furin Antibody [NB100-1903] - Analysis was performed on membrane enriched extracts (30 ug lysate) of HeLa (Lane 1), HEK 293 (Lane 2), K-562 (Lane 3), SH-SY5Y (Lane 4) and 10uL conditioned media from HeLa cell line (Lane 5).](http://images.novusbio.com/images2/Furin-Antibody-Western-Blot-NB100-1903-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: Furin Antibody [NB100-1903] - Immunolocalization of endogenous furin in mouse 3T3 cells.](http://images.novusbio.com/images2/Furin-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1903-img0005.jpg)
![Flow Cytometry: Furin Antibody [NB100-1903] - Flow cytometry analysis of Furin Convertase was done on HeLa cells. Cells were fixed with 70% ethanol for 10 minutes, permeabilized with 0.25% Triton (R) X-100 for 20 minutes, and blocked with 5% BSA for 30 minutes at room temperature. Cells were labeled with Furin Convertase Rabbit Polyclonal Antibody or with rabbit isotype control (pink histogram) at 3-5 ug/million cells in 2.5% BSA. After incubation at room temperature for 2 hours, the cells were labeled with Alexa Fluor (R) 488 Goat Anti-Rabbit Secondary Antibody (A11008) at a dilution of 1:400 for 30 minutes at room temperature. The representative 10, 000 cells were acquired and analyzed for each sample using an Attune (R) Acoustic Focusing Cytometer. The purple histogram represents unstained control cells and the green histogram represents no-primary-antibody control.](http://images.novusbio.com/images2/Furin-Antibody-Flow-Cytometry-NB100-1903-img0007.jpg)
![Western Blot: Proprotein Convertase 1/PCSK1 Antibody (3D2) [H00005122-M02] - PCSK1 monoclonal antibody (M02), clone 3D2. Analysis of PCSK1 expression in Raw 264.7.](http://images.novusbio.com/images2/Proprotein-Convertase-1-PCSK1-Antibody-(3D2)-Western-Blot-H00005122-M02-img0016.jpg)
![Immunocytochemistry/Immunofluorescence: Proprotein Convertase 1/PCSK1 Antibody (3D2) [H00005122-M02] - Analysis of monoclonal antibody to PCSK1 on HeLa cell. Antibody concentration 30 ug/ml.](http://images.novusbio.com/images2/Proprotein-Convertase-1-PCSK1-Antibody-(3D2)-Immunocytochemistry-Immunofluorescence-H00005122-M02-img0010.jpg)
![Immunohistochemistry-Paraffin: Proprotein Convertase 1/PCSK1 Antibody (3D2) [H00005122-M02] - Analysis of monoclonal antibody to PCSK1 on formalin-fixed paraffin-embedded human small Intestine. Antibody concentration 3 ug/ml.](http://images.novusbio.com/images2/Proprotein-Convertase-1-PCSK1-Antibody-(3D2)-Immunohistochemistry-Paraffin-H00005122-M02-img0012.jpg)
![Western Blot: PACE4 Antibody [H00005046-A01] - Analysis of PCSK6 expression in PC-12.](http://images.novusbio.com/images2/PACE4-Antibody-Western-Blot-H00005046-A01-img0004.jpg)
![Western Blot: PACE4 Antibody [H00005046-A01] - Analysis of PCSK6 expression in Hela S3 NE (Cat # L013V3 ).](http://images.novusbio.com/images2/PACE4-Antibody-Western-Blot-H00005046-A01-img0002.jpg)
![Western Blot: PACE4 Antibody [H00005046-A01] - Analysis of PCSK6 expression in NIH/3T3.](http://images.novusbio.com/images2/PACE4-Antibody-Western-Blot-H00005046-A01-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Proprotein Convertase 7/PCSK7 Antibody (OTI1B8) - Azide and BSA Free [NBP2-73621] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY PCSK7.](http://images.novusbio.com/images2/Proprotein-Convertase-7-PCSK7-Antibody-(OTI1B8)---Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-73621-img0003.jpg)
![Flow Cytometry: Proprotein Convertase 7/PCSK7 Antibody (OTI1B8) - Azide and BSA Free [NBP2-73621] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-PCSK7 antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/images2/Proprotein-Convertase-7-PCSK7-Antibody-(OTI1B8)---Azide-and-BSA-Free-Flow-Cytometry-NBP2-73621-img0002.jpg)
![Flow Cytometry: Proprotein Convertase 7/PCSK7 Antibody (OTI1B8) - Azide and BSA Free [NBP2-73621] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-PCSK7 antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/images2/Proprotein-Convertase-7-PCSK7-Antibody-(OTI1B8)---Azide-and-BSA-Free-Flow-Cytometry-NBP2-73621-img0001.jpg)
![Western Blot: Cytokeratin 17 Antibody (E3 (same as Ks17.E3)) [NBP2-29421] - Analysis of Cytokeratin 17 in human HeLa lysate using Cytokeratin 17 E3 antibody at 3 ug/ml. goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/images2/Cytokeratin-17-Antibody-(E3-(same-as-Ks17.E3))-Western-Blot-NBP2-29421-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Cytokeratin 17 Antibody (E3 (same as Ks17.E3)) [NBP2-29421] - Formalin-paraffin skin stained with CK17 Ab (E3).](http://images.novusbio.com/images2/Cytokeratin-17-Antibody-(E3-(same-as-Ks17.E3))-Immunocytochemistry-Immunofluorescence-NBP2-29421-img0005.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 17 Antibody (E3 (same as Ks17.E3)) [NBP2-29421] - Formalin-paraffin skin stained with CK17 Ab (E3).](http://images.novusbio.com/images2/Cytokeratin-17-Antibody-(E3-(same-as-Ks17.E3))-Immunohistochemistry-Paraffin-NBP2-29421-img0004.jpg)
![Western Blot: CBX4 Antibody (6C5G3) [NBP2-52432] - Analysis using CBX4 mAb against human CBX4 (AA: 397-514) recombinant protein. (Expected MW is 43.3 kDa)](http://images.novusbio.com/images2/CBX4-Antibody-(6C5G3)-Western-Blot-NBP2-52432-img0006.jpg)
![Immunohistochemistry-Paraffin: CBX4 Antibody (6C5G3) [NBP2-52432] - Analysis of rectum cancer tissues using CBX4 mouse mAb with DAB staining.](http://images.novusbio.com/images2/CBX4-Antibody-(6C5G3)-Immunohistochemistry-NBP2-52432-img0003.jpg)
![Flow Cytometry: CBX4 Antibody (6C5G3) [NBP2-52432] - Flow cytometric analysis of Hela cells using CBX4 mouse mAb (green) and negative control (red).](http://images.novusbio.com/images2/CBX4-Antibody-(6C5G3)-Flow-Cytometry-NBP2-52432-img0001.jpg)
![Western Blot: Polycystin 2 Antibody [NBP1-30117] - Whole cell lysate (15 ug) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Polycystin-2 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/images2/Polycystin-2-Antibody-Western-Blot-NBP1-30117-img0007.jpg)
![Western Blot: Polycystin 2 Antibody [NBP1-30117] - Whole cell lysate (50 ug) from TCMK-1 and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Polycystin-2 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.](http://images.novusbio.com/images2/Polycystin-2-Antibody-Western-Blot-NBP1-30117-img0006.jpg)
![Immunoprecipitation: Polycystin 2 Antibody [NBP1-30117] - Detection of human Polycystin-2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Polycystin-2 antibody NBP1-30117 used for IP at 3 ug per reaction. Polycystin-2 was also immunoprecipitated by another rabbit anti-Polycystin-2 antibody. For blotting immunoprecipitated Polycystin-2, NBP1-30117 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/images2/Polycystin-2-Antibody-Immunoprecipitation-NBP1-30117-img0005.jpg)
![Western Blot: Cytokeratin 6b Antibody [NBP2-14924] - Sample (1 ug of whole cell lysate) A: HeLa 7. 5% SDS PAGE; antibody diluted at 1:5000.](http://images.novusbio.com/images2/Cytokeratin 6b Antibody-Western Blot-NBP2-14924-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Cytokeratin 6b Antibody [NBP2-14924] - Analysis of methanol-fixed HeLa, using antibody at 1:500 dilution.](http://images.novusbio.com/images2/Cytokeratin 6b Antibody-Immunocytochemistry-Immunofluorescence-NBP2-14924-img0001.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 6b Antibody [NBP2-14924] - Paraffin-embedded mouse brain, using antibody at 1:500 dilution.](http://images.novusbio.com/images2/Cytokeratin 6b Antibody-Immunohistochemistry-Paraffin-NBP2-14924-img0002.jpg)
![Immunohistochemistry-Paraffin: ENPP-1 Antibody [NBP2-27561] - Negative Control showing staining of paraffin embedded Human Thyroid Gland, with no primary antibody.](http://images.novusbio.com/images2/ENPP-1-Antibody-Immunohistochemistry-Paraffin-NBP2-27561-img0003.jpg)
![Immunohistochemistry-Paraffin: ENPP-1 Antibody [NBP2-27561] - Staining of paraffin embedded Human Liver. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.](http://images.novusbio.com/images2/ENPP-1-Antibody-Immunohistochemistry-Paraffin-NBP2-27561-img0001.jpg)
![Immunohistochemistry-Paraffin: ENPP-1 Antibody [NBP2-27561] - Staining of paraffin embedded Human Thyroid Gland at dilution of 8ug/ml. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.](http://images.novusbio.com/images2/ENPP-1-Antibody-Immunohistochemistry-Paraffin-NBP2-27561-img0002.jpg)
![Immunohistochemistry-Paraffin: Proprotein convertase PC4 Antibody [NBP1-88010] - Staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts and Leydig cells.](http://images.novusbio.com/images2/Proprotein-convertase-PC4-Antibody-Immunohistochemistry-Paraffin-NBP1-88010-img0004.jpg)
![Immunohistochemistry: Proprotein convertase PC4 Antibody [NBP1-88010] - Staining of human cerebral cortex shows strong cytoplasmic positivity in granular pattern in neuronal cells.](http://images.novusbio.com/images2/Proprotein-convertase-PC4-Antibody-Immunohistochemistry-NBP1-88010-img0003.jpg)
![Western Blot: SUB1 Antibody [NBP1-82454] - Analysis in human cell line RPMI-8226.](http://images.novusbio.com/images2/SUB1-Antibody-Western-Blot-NBP1-82454-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: SUB1 Antibody [NBP1-82454] - Staining of human cell line U-2 OS shows localization to nucleus & nucleoli. Antibody staining is shown in green.](http://images.novusbio.com/images2/SUB1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-82454-img0011.jpg)
![Immunohistochemistry-Paraffin: SUB1 Antibody [NBP1-82454] - Staining of human cerebellum shows strong nuclear positivity in Purkinje cells.](http://images.novusbio.com/images2/SUB1-Antibody-Immunohistochemistry-Paraffin-NBP1-82454-img0015.jpg)
![Western Blot: IFRD1 Antibody [NBP2-24679] - Analysis of Tis7 in HeLa cell lysate in the 1) absence 2) presence of immunizing peptide and 3) RAW cell lysate using this antibody. 0 ug/ml, 1.0 ug/ml and 4.0 ug/ml respectively.](http://images.novusbio.com/images2/IFRD1 Antibody-Western Blot-NBP2-24679-img0002.jpg)
![Immunohistochemistry-Paraffin: IFRD1 Antibody [NBP2-24679] - Immunohistochemical analysis of IFRD1 in formalin-fixed, paraffin-embedded humanrectum tissue using an isotype control (top) and NBP2-24679 (bottom) at 5 ug/ml.](http://images.novusbio.com/images2/IFRD1 Antibody-Immunohistochemistry-Paraffin-NBP2-24679-img0001.jpg)
![Western Blot: Cbx8 Antibody [NBP1-83224] - Analysis in human cell line A-549.](http://images.novusbio.com/images2/Cbx8-Antibody-Western-Blot-NBP1-83224-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: Cbx8 Antibody [NBP1-83224] - Staining of human cell line A-431 shows localization to nucleoplasm. Antibody staining is shown in green.](http://images.novusbio.com/images2/Cbx8-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-83224-img0008.jpg)
![Immunohistochemistry-Paraffin: Cbx8 Antibody [NBP1-83224] - Analysis in human testis and liver tissues. Corresponding CBX8 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/images2/Cbx8-Antibody-Immunohistochemistry-Paraffin-NBP1-83224-img0013.jpg)
![Western Blot: ACTR1B Antibody [NBP2-15275] - Sample (30 ug of whole cell lysate) A: IMR32 10% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/images2/ACTR1B Antibody-Western Blot-NBP2-15275-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: ACTR1B Antibody [NBP2-15275] - SKNSH cells were fixed in ice-cold MeOH for 5 min. Green: ACTR1B protein stained by ACTR1B antibody diluted at 1:1000. Blue: Hoechst 33342 staining.](http://images.novusbio.com/images2/ACTR1B-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-15275-img0004.jpg)
![Immunohistochemistry-Paraffin: ACTR1B Antibody [NBP2-15275] - Paraffin-embedded ovarian carcinoma. ACTR1B antibody dilution: 1:500.](http://images.novusbio.com/images2/ACTR1B-Antibody-Immunohistochemistry-Paraffin-NBP2-15275-img0003.jpg)
![Western Blot: BTG1 Antibody (4C10) [NBP2-46587] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BTG 1.](http://images.novusbio.com/images2/BTG1 Antibody (4C10)-Western Blot-NBP2-46587-img0006.jpg)
![Immunohistochemistry: BTG1 Antibody (4C10) [NBP2-46587] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/images2/BTG1-Antibody-(4C10)-Immunohistochemistry-NBP2-46587-img0005.jpg)
![Immunohistochemistry: BTG1 Antibody (4C10) [NBP2-46587] - Analysis of Human colon tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/images2/BTG1-Antibody-(4C10)-Immunohistochemistry-NBP2-46587-img0001.jpg)
![Western Blot: MBTPS1 Antibody [NBP3-05160] - Paraffin-embedded human colon using MBTPS1 antibody at dilution of 1:100 (40x lens).](http://images.novusbio.com/images2/MBTPS1-Antibody-Western-Blot-NBP3-05160-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: MBTPS1 Antibody [NBP3-05160] - Analysis of U2OS cells using MBTPS1 antibody. Blue: DAPI for nuclear staining.](http://images.novusbio.com/images2/MBTPS1-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-05160-img0001.jpg)
![Immunohistochemistry-Paraffin: MBTPS1 Antibody [NBP3-05160] - Paraffin-embedded human stomach using MBTPS1 antibody at dilution of 1:100 (40x lens).](http://images.novusbio.com/images2/MBTPS1 Antibody-Immunohistochemistry-Paraffin-NBP3-05160-img0004.jpg)
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - An intracellular stain was performed on Raji cells with Adiponectin antibody NB100-65810 (blue) and a matched isotype control NBP2-24893 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Dylight488-conjugated anti-rabbit secondary antibody. Image using the Azide Free form of this antibody.](http://images.novusbio.com/images2/Rabbit-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0006.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - An intracellular stain was performed on Raji cells with Adiponectin antibody NB100-65810 (blue) and a matched isotype control NBP2-24893 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Dylight488-conjugated anti-rabbit secondary antibody. Image using the Azide Free form of this antibody.")