SPAG8 Antibody (2F12)

Images

 
Western Blot: SPAG8 Antibody (2F12) [H00026206-M01] - Analysis of SPAG8 expression in transfected 293T cell line by SPAG8 monoclonal antibody (M01), clone 2F12.Lane 1: SPAG8 transfected lysate(53.5 KDa).Lane 2: ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA
Clone
2F12
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

SPAG8 Antibody (2F12) Summary

Immunogen
SPAG8 (AAH19247, 1 a.a. ~ 502 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
SPAG8 - sperm associated antigen 8 (2F12)
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
SPAG8
Purity
IgG purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:500
  • ELISA
Application Notes
Antibody reactive against transfected lysate and recombinant protein for Western Blot. Has also been used for ELISA.

Reactivity Notes

Human.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for SPAG8 Antibody (2F12)

  • BS-84
  • HSD-1MGC26201
  • hSMP-1
  • SMP1
  • SMP-1
  • SPAG3
  • sperm associated antigen 8
  • Sperm membrane protein 1
  • Sperm membrane protein BS-84
  • sperm-associated antigen 8

Background

The correlation of anti-sperm antibodies with cases of unexplained infertility implicates a role for these antibodies in blocking fertilization. Improved diagnosis and treatment of immunologic infertility, as well as identification of proteins for targeted contraception, are dependent on the identification and characterization of relevant sperm antigens. The protein encoded by this gene is recognized by sperm agglutinating antibodies from an infertile woman. This protein is localized in germ cells of the testis at all stages of spermatogenesis and is localized to the acrosomal region of mature spermatozoa. Alternatively spliced variants that encode different protein isoforms have been described but the full-length sequences of only two have been determined. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol SPAG8
Entrez
Uniprot