SP110 Antibody (8C8) - Azide and BSA Free

Western Blot: SP110 Antibody (8C8) [H00003431-M01] - Analysis of SP110 expression in transfected 293T cell line by SP110 monoclonal antibody (M01), clone 8C8.Lane 1: SP110 transfected lysate(61.8 KDa).Lane 2: Non-transfected lysate.

Immunohistochemistry-Paraffin: SP110 Antibody (8C8) [H00003431-M01] - Analysis of monoclonal antibody to SP110 on formalin-fixed paraffin-embedded human small Intestine. Antibody concentration 3 ug/ml.

Western Blot: SP110 Antibody (8C8) [H00003431-M01] - SP110 expression in Jurkat ( Cat # L017V1 ).

Western Blot: SP110 Antibody (8C8) [H00003431-M01] - Analysis of SP110 over-expressed 293 cell line, cotransfected with SP110 Validated Chimera RNAi ( Cat # H00003431-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with SP110 monoclonal antibody (M01), clone 8C8 (Cat # H00003431-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.

Immunoprecipitation: SP110 Antibody (8C8) [H00003431-M01] - Analysis of SP110 transfected lysate using anti-SP110 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with SP110 MaxPab rabbit polyclonal antibody.

ELISA: SP110 Antibody (8C8) [H00003431-M01] - Detection limit for recombinant GST tagged SP110 is approximately 0.1ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, IHC, IP, KD
• Clone: 8C8
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

SP110 Antibody (8C8) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: SP110 (NP_004501, 271 a.a. ~ 380 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. TPSDKKGKKRKRCIWSTPKRRHKKKSLPRGTASSRHGIQKKLKRVDQVPQKKDDSTCNSTVETRAQKARTECARKSRSEEIIDGTSEMNEGKRSQKTPSTPRRVTQGAAS
• Specificity: SP110 (8C8)
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: SP110
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • Knockdown Validated
  • Western Blot 1:500
• Application Notes: Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IHC-P, RNAi Validation and ELISA.
• Control:

  HeLa Nuclear Cell Lysate

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: Protein A purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for SP110 Antibody (8C8) - Azide and BSA Free

• FLJ22835
• IFI41
• IFI75
• interferon-induced protein 41, 30kD
• Interferon-induced protein 41/75
• interferon-induced protein 75, 52kD
• IPR1
• phosphoprotein 41
• phosphoprotein 75
• SP110 nuclear body protein
• Speckled 110 kDa
• Transcriptional coactivator Sp110
• VODI

Background

The nuclear body is a multiprotein complex that may have a role in the regulation of gene transcription. This gene is a member of the SP100/SP140 family of nuclear body proteins and encodes a leukocyte-specific nuclear body component. The protein can function as an activator of gene transcription and may serve as a nuclear hormone receptor coactivator. In addition, it has been suggested that the protein may play a role in ribosome biogenesis and in the induction of myeloid cell differentiation. Alternative splicing has been observed for this gene and three transcript variants, encoding distinct isoforms, have been identified.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SP110
• Entrez:
  • 3431(Human)
  • 109032(Mouse)
  • 301570(Rat)
• OMIM:
  • 604457(Human)
• Uniprot:
  • NP_004501(Human)