SOX21 Antibody (CL4688)

Images

 
Western Blot: SOX21 Antibody (CL4688) [NBP2-59059] - Analysis in human cell line NTERA-2.
Immunocytochemistry/ Immunofluorescence: SOX21 Antibody (CL4688) [NBP2-59059] - Staining of PC-3 cells using the Anti-SOX21 monoclonal antibody, showing specific staining in the nucleus in green. Microtubule- and ...read more
Immunohistochemistry-Paraffin: SOX21 Antibody (CL4688) [NBP2-59059] - Staining of human fallopian tube shows no positivity as expected (negative control).
Immunohistochemistry-Paraffin: SOX21 Antibody (CL4688) [NBP2-59059] - Staining of mouse embryo E14 shows nuclear immunoreactivity in the developing forebrain.
Immunohistochemistry-Paraffin: SOX21 Antibody (CL4688) [NBP2-59059] - Staining of mouse forebrain shows nuclear immunoreactivity in a subset of cells in the lateral ventricle wall.
Immunohistochemistry-Paraffin: SOX21 Antibody (CL4688) [NBP2-59059] - Staining of mouse cerebral cortex shows nuclear positivity in a subset of cells.
Immunohistochemistry-Paraffin: SOX21 Antibody (CL4688) [NBP2-59059] - Staining of human stomach shows moderate nuclear positivity in a subset of glandular cells.
Immunohistochemistry-Paraffin: SOX21 Antibody (CL4688) [NBP2-59059] - Staining of human cervix shows moderate nuclear immunoreactivity in a subset of squamous epithelial cells.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clone
CL4688
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

SOX21 Antibody (CL4688) Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to the following amino acid sequence:GKPQLDPYPAAYAAAL
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
SOX21
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 2-10 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Western Blot 1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. For ICC/IF, PFA/Triton X-100 is recommended for fixation/permeabilization.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Protein A purified

Alternate Names for SOX21 Antibody (CL4688)

  • SOX21
  • SOX25
  • SOX25SOX-A
  • SOXA
  • SRY (sex determining region Y)-box 21
  • SRY-box 21
  • transcription factor SOX-21

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for SOX21 Antibody (NBP2-59059) (0)

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Secondary Antibodies

 

Isotype Controls

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Array NBP2-59059

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Bioinformatics

Gene Symbol SOX21