SOX11 Antibody (1106960) [Unconjugated]

SOX11 was detected in immersion fixed paraffin-embedded sections of human Mantle Cell Lymphoma using Mouse Anti-Human SOX11, Monoclonal Antibody (Catalog # MAB11734) at 25ug/mL at 37° Celsius for 8 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ 647 Goat anti-Mouse IgG Secondary Antibody at 1:200 at 37 ° Celsius for 8 minutes. (Yellow; Lunaphore Catalog # DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100).​ Specific staining was localized to the nucleus. Protocol available in COMET™ Panel Builder.

SOX11 was detected in immersion fixed paraffin-embedded sections of human glioblastoma using Mouse Anti-Human SOX11 Monoclonal Antibody (Catalog # MAB11734) at 5 µg/ml for 1 hour at room temperature followed by incubation with the HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the nucleus. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

SOX11 was detected in immersion fixed paraffin-embedded sections of human mantle cell lymphoma using Mouse Anti-Human SOX11 Monoclonal Antibody (Catalog # MAB11734) at 15 µg/ml overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using the HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007) and counterstained with hematoxylin (blue). Specific staining was localized to the nucleus. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Western Blot shows lysates of SH‑SY5Y human neuroblastoma cell line. PVDF membrane was probed with 2 µg/ml of Mouse Anti-Human SOX11 Monoclonal Antibody (Catalog # MAB11734) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for SOX11 at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

• Reactivity: Hu
• Applications: WB, IHC
• Clone: 1106960
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

SOX11 Antibody (1106960) [Unconjugated] Summary

• Immunogen: E. coli - derived recombinant human SOX-11
  Met1-Pro173
  Accession # P35716
• Specificity: Detects recombinant human SOX11 protein in Direct ELISA.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 3-25 ug/mL
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Reconstitution Instructions: Reconstitute lyophilized material at 0.2 mg/ml in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for SOX11 Antibody (1106960) [Unconjugated]

• SOX11
• SRY (sex determining region Y)-box 11
• SRY (sex-determining region Y)-box 11
• SRY-related HMG-box gene 11
• transcription factor SOX-11

Background

Human SOX11 (SRY box-related gene 11) is a 60-72 kDa (46.6 kDa predicted), group C member of the SOX family of transcription factors. It is 441 amino acids (aa) in length, and contains one HMG box (aa 49-117), two poly-Gly regions (aa 144-214), one poly-Asp domain (aa 223-233) and one poly-Ser region. The HMG box determines DNA and transcriptional coactivator binding, the poly-Ser region lies in a potent transactivating region, and the poly-Asp domain autoinhibits/regulates activities of the other two regions. SOX11 appears early in development in multiple tissues, and impacts neuron, oligodendrocyte and cartilage formation. Human SOX11 is 94% aa identical to mouse SOX11 over aa 1-173. Mouse SOX11, however, is absent a 12 aa poly-Gly sequence that is present in human SOX11 between aa 145-156. Additional band correspondent to the lower molecular weight may represent SOX11 without posttranslational modifications or human SOX4, over  aa 38-123 human SOX11 shares 100% aa identity with human SOX4. (1)

1. Dictor, M. et al. (2009) Haematologica 94:1563.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.