SOX1 Antibody (340303)

Western blot shows lysates of MCF-7 human breast cancer cell line, HeLa human cervical epithelial carcinoma cell line, NIH-3T3 mouse embryonic fibroblast cell line, and DA3 mouse myeloma cell line. PVDF membrane was probed with 0.1 µg/mL of Human/Mouse SOX1 Monoclonal Antibody (Catalog # MAB3369) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for SOX1 at approximately 38 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.

Product Discontinued

SOX1 Antibody (340303) Summary

• Immunogen: E. coli-derived recombinant human SOX1
  Asn242-Gly379 (Leu276Ile)
  Accession # O00570
• Specificity: Detects endogenous human and mouse SOX1 in Western blots. In Western blots, this antibody shows approximately 10% cross-reactivity with rhSOX-2, and no cross-reactivity with rhSOX-3, -5, -6, -7, -9, -10, -11, -15, -17, or rmSOX-21.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: SOX1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for SOX1 Antibody (340303)

• SOX1
• SRY (sex determining region Y)-box 1
• SRY-related HMG-box gene 1
• transcription factor SOX-1

Background

SOX1 (SRY-related HMG box 1) is a 39 kDa transcription factor that belongs to the SRY family of HMG box proteins of which there are 30 members in vertebrates. Group B contains SOX1 along with SOX2, SOX3, SOX14 and SOX21; each of these factors share greater than 90% homology in their respective HMG box region. SOX1 maintains neural cells in an undifferentiated state and has been used as a marker for neural stem cells. Human and mouse SOX1 share 97% amino acid sequence identity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SOX1 Antibody (MAB3369)(2)

Publications using MAB3369

• La Fleur L, Johansson A, Roberg K A CD44(high)/EGFR(low) Subpopulation within Head and Neck Cancer Cell Lines Shows an Epithelial-Mesenchymal Transition Phenotype and Resistance to Treatment. PLoS ONE, 2012-09-25;7(9):e44071. 2012-09-25 [PMID: 23049743] (Western Blot, Human)
• Cho A, Ko HW, Eggenschwiler JT FKBP8 cell-autonomously controls neural tube patterning through a Gli2- and Kif3a-dependent mechanism. Dev. Biol., 2008-06-11;321(1):27-39. 2008-06-11 [PMID: 18590716] (IHC-Fr, Mouse)

Bioinformatics

• Gene Symbol: SOX1
• Uniprot:
  • O00570()