Sodium Potassium ATPase Alpha 3 Antibody (XVIF9-G10)

Images

 
Immunocytochemistry/ Immunofluorescence: Sodium Potassium ATPase Alpha 3 Antibody (XVIF9-G10) [NB300-540] - Sodium/Potassium ATPase alpha-3 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI ...read more
Immunohistochemistry-Paraffin: Sodium Potassium ATPase Alpha 3 Antibody (XVIF9-G10) [NB300-540] - Both normal and cancer biopsies of deparaffinized Human prostate carcinoma tissues.
Immunocytochemistry/ Immunofluorescence: Sodium Potassium ATPase Alpha 3 Antibody (XVIF9-G10) [NB300-540] - Analysis of Sodium/Potassium ATPase alpha-3 using Sodium/Potassium ATPase alpha-3 Monoclonal antibody ...read more
Immunocytochemistry/ Immunofluorescence: Sodium Potassium ATPase Alpha 3 Antibody (XVIF9-G10) [NB300-540] - Sodium/Potassium ATPase alpha-3 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI ...read more
Immunohistochemistry-Paraffin: Sodium Potassium ATPase Alpha 3 Antibody (XVIF9-G10) [NB300-540] - Both normal and cancer biopsies of deparaffinized Human tonsil tissues.
Immunohistochemistry-Paraffin: Sodium Potassium ATPase Alpha 3 Antibody (XVIF9-G10) [NB300-540] - Both normal and cancer biopsies of deparaffinized Human colon tissues.
Simple Western: Sodium Potassium ATPase Alpha 3 Antibody (XVIF9-G10) [NB300-540] - Simple Western lane view shows a specific band for Sodium Potassium ATPase Alpha 3 in 0.5 mg/ml of Human Brain lysate. This experiment ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, Ca, Gp, Pm, Rb, ShSpecies Glossary
Applications WB, Simple Western, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, B/N, Single-Cell Western
Clone
XVIF9-G10
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

Sodium Potassium ATPase Alpha 3 Antibody (XVIF9-G10) Summary

Immunogen
Canine cardiac microsomes.
Marker
Plasma Membrane Marker
Specificity
Detects sodium / potassium ATPase. This is specific for the alpha-3 subunit.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
ATP1A3
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Simple Western 1:100
  • Flow Cytometry 1 ug / 10^6 cells
  • Immunocytochemistry/Immunofluorescence 5 ug/mL
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Frozen 3 ug/mL
  • Immunohistochemistry-Paraffin 1:10 - 1:500
  • Block/Neutralize
  • Single Cell Western 100 ug/mL
Application Notes
May be useful in WB, IF, and IHC-Fr. WB: Detects an approx. 110 kDa protein representing the alpha-3 subunit of the sodium/potassium ATPase from canine skeletal muscle extract. IHC: Staining of sodium / potassium ATPase in rat retina yields a pattern consistent with plasma membrane localization.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Single Cell Western validated using live primary humna cortical neurons.
Theoretical MW
111.749 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
Sodium Potassium ATPase Alpha 3 Overexpression Lysate
Reviewed Applications
Read 2 Reviews rated 4
using
NB300-540 in the following applications:

Publications
Read Publications using
NB300-540 in the following applications:

  • 1 publication
  • 2 publications
  • IHC
    3 publications
  • WB
    4 publications

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.02 M potassium phosphate (pH 7.2), 0.15 M NaCl
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A purified

Alternate Names for Sodium Potassium ATPase Alpha 3 Antibody (XVIF9-G10)

  • alpha 3 polypeptide
  • ATPase, Na+/K+ transporting, alpha 3 polypeptide
  • dystonia 12
  • DYT12
  • EC 3.6.3
  • MGC13276
  • RDP
  • Sodium pump subunit alpha-3
  • sodium/potassium-transporting ATPase alpha-3 chain
  • sodium/potassium-transporting ATPase subunit alpha-3

Background

The sodium/potassium ATPase is an integral membrane enzyme found in all cells of higher organisms and is responsible for the ATP-dependent transport of sodium and potassium across the cell membrane. This membrane-bound enzyme is related to a number of other ATPases including sarcoplasmic and endoplasmic reticulum calcium ATPase (SERCA) and plasma membrane calcium ATPase (PMCA). The sodium/potassium ATPase consists of a large, multipass, transmembrane catalytic subunit, termed the alpha subunit, and an associated smaller glycoprotein, termed the beta subunit. Studies indicate that there are three isoforms of the alpha subunit (alpha 1, alpha 2, alpha 3) and two isoforms of the beta subunit (beta 1 and beta 2) encoded by two multigene families. The different isoforms of the sodium/potassium ATPase exhibit tissue-specific and developmental patterns of expression. The alpha 1 and beta mRNAs are present in all cell types examined, whereas the alpha 2 and alpha 3 mRNAs exhibit a more restricted pattern of cell-specific expression. The alpha-3 subunit has been found in neuronal and to skeletal and cardiac muscle, lung and stomach tissues.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Sodium Potassium ATPase Alpha 3 Antibody (NB300-540)(15)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 4 applications: Flow, ICC/IF, IHC, WB.


Filter By Application
Flow
(1)
ICC/IF
(2)
IHC
(3)
WB
(4)
All Applications
Filter By Species
Human
(1)
Mouse
(1)
All Species
Showing Publications 1 - 10 of 15. Show All 15 Publications.
Publications using NB300-540 Applications Species
Coleman LG, Zou J, Crews FT. Microglial-derived miRNA let-7 and HMGB1 contribute to ethanol-induced neurotoxicity via TLR7 J Neuroinflammation Jan 25 2017 [PMID: 28118842] (FLOW, Human). J Neuroinflammation. 2017 Jan 25 [PMID: 28118842] (Flow, Human)

Details:
This citation used the Allophycocyanin version of this antibody.
Flow Human
Ivakine EA, Acton BA, Mahadevan V et al. Neto2 is a KCC2 interacting protein required for neuronal Cl- regulation in hippocampal neurons. Proc Natl Acad Sci U S A 2013 Feb 26 [PMID: 23401525] (WB, Mouse) WB Mouse
Rose EM, Koo JCP, Antflick JE et al. Glutamate Transporter Coupling to Na,K-ATPase. J Neurosci;29(25):8143-8155. 2009 [PMID: 19553454]
Hou Z Matherly LH. Oligomeric structure of the human reduced folate carrier: Identification of homo-oligomers dominant-negative effects on carrier expression function. J Biol Chem :M807206200. 2008 [PMID: 19019821]
Ferguson SM, Savchenko V, Apparsundaram S, Zwick M, Wright J, Heilman CJ, Yi H, Levey AI, Blakely RD. Vesicular localization and activity-dependent trafficking of presynaptic choline transporters. J Neurosci;23(30):9697-709. 2003 Oct 29. [PMID: 14585997] (IHC) IHC
Hoffman JF, Wickrema A, Potapova O, Milanick M, Yingst DR. Na pump isoforms in human erythroid progenitor cells and mature erythrocytes. Proc Natl Acad Sci U S A;99(22):14572-7. 2002 Oct 29. [PMID: 12388775] (ICC/IF) ICC/IF
Pfeiffer R, Beron J, Verrey F. Regulation of Na+ pump function by aldosterone is alpha-subunit isoform specific. J Physiol;516 ( Pt 3)():647-55. 1999 May 1. (WB) WB
Molday LL, Wu WW, Molday RS. Retinoschisin (RS1), the protein encoded by the X-linked retinoschisis gene, is anchored to the surface of retinal photoreceptor and bipolar cells through its interactions with a Na/K ATPase-SARM1 complex. J Biol Chem;282(45):32792-801. 2007 Nov 9. [PMID: 17804407] (WB) WB
Brickley DR, Mikosz CA, Hagan CR, Conzen SD. Ubiquitin modification of serum and glucocorticoid-induced protein kinase-1 (SGK-1). J Biol Chem;277(45):43064-70. 2002 Nov 8. [PMID: 12218062] (ICC/IF) ICC/IF
Kong JQ, Meng J, Biser PS, Fleming WW, Taylor DA. Cellular depolarization of neurons in the locus ceruleus region of the guinea pig associated with the development of tolerance to opioids. J Pharmacol Exp Ther;298(3):909-16. 2001 Sep. [PMID: 11504784] (IHC) IHC
Show All 15 Publications.

Reviews for Sodium Potassium ATPase Alpha 3 Antibody (NB300-540) (2) 42

Average Rating: 4
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Human, Mouse.

Reviews using NB300-540:
Filter by Applications
WB
(2)
All Applications
Filter by Species
Human
(1)
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
  3
reviewed by:
Verified Customer
WB Human 08/22/2015
View

Summary

ApplicationWestern Blot
Sample TestedHuman cancer cell
SpeciesHuman

Blocking

Blocking DetailsBlocking with 4% BSA for 30 min

Primary Anitbody

Dilution Ratio1:1000, overnight

Secondary Antibody

Secondary Concentration1:2000

Details

Detection NotesRegular Protocol
  5
reviewed by:
Vivek Mahadevan
WB Mouse 08/21/2012
View

Summary

ApplicationWestern Blot
Sample TestedMembrane fraction from whole brain
SpeciesMouse
LotME 158235
CommentsDetects endogenous ATP1A3 quite well from brain lysates

Blocking

Blocking Details3% non-fat dry milk in TBST, for 1hr @ room temperature

Primary Anitbody

Dilution Ratio1:1000 dilution in blocking buffer, incubated for 4hrs @ room temperature

Secondary Antibody

Secondary DescriptionHRP-conjugated affinity purified horse anti-mouse IgG (H&L)
Secondary Manufacturer Cat#Cell signaling # 7076
Secondary Concentration1:5000 ilution in blocking buffer, incubated for 1hrs @ room temperature

Details

Detection NotesECL detections, 2 min exposure time using a blue X-ray film

Comments

CommentsDetects endogenous ATP1A3 quite well from brain lysates

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Sodium Potassium ATPase Alpha 3 Antibody (NB300-540) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB300-540AF350
Alexa Fluor 405 NB300-540AF405
Alexa Fluor 488 NB300-540AF488
Alexa Fluor 532 NB300-540AF532
Alexa Fluor 594 NB300-540AF594
Alexa Fluor 647 NB300-540AF647
Alexa Fluor 700 NB300-540AF700
Alexa Fluor 750 NB300-540AF750
Allophycocyanin NB300-540APC
Allophycocyanin/Cy7 NB300-540APCCY7
Biotin NB300-540B
DyLight 350 NB300-540UV
DyLight 405 NB300-540V
DyLight 488 NB300-540G
DyLight 594 NB300-540DL594
DyLight 650 NB300-540C
DyLight 680 NB300-540FR
DyLight 755 NB300-540IR
HRP NB300-540HRP
Janelia Fluor 549 NB300-540JF549
Janelia Fluor 646 NB300-540JF646
PE NB300-540PE
PE/Atto594 NB300-540PEATT594
PE/Cy5.5 NB300-540PECY55
PE/Cy7 NB300-540PECY7

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Recent Reviews

4
5
1
4
0
3
1
2
0
1
0

 
Verified Customer
08/22/2015
Application: WB
Species: Human

 
Vivek Mahadevan
08/21/2012
Application: WB
Species: Mouse

Bioinformatics

Gene Symbol ATP1A3
Entrez
Uniprot