Sodium Potassium ATPase Alpha 3 Antibody (XVIF9-G10) - BSA Free

Immunocytochemistry/Immunofluorescence: Sodium Potassium ATPase Alpha 3 Antibody (XVIF9-G10) [NB300-540] - Sodium/Potassium ATPase alpha-3 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) in U251 glioma cells is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Sodium/Potassium ATPase alpha-3 at a dilution of 1:20 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.

Immunohistochemistry-Paraffin: Sodium Potassium ATPase Alpha 3 Antibody (XVIF9-G10) [NB300-540] - Both normal and cancer biopsies of deparaffinized Human prostate carcinoma tissues.

Immunocytochemistry/Immunofluorescence: Sodium Potassium ATPase Alpha 3 Antibody (XVIF9-G10) [NB300-540] - Analysis of Sodium/Potassium ATPase alpha-3 using Sodium/Potassium ATPase alpha-3 Monoclonal antibody (XVIF9-G10) shows staining in C6 glioma cells. Sodium/Potassium ATPase alpha-3 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Sodium/Potassium ATPase alpha-3 at a dilution of 1:20 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.

Immunocytochemistry/Immunofluorescence: Sodium Potassium ATPase Alpha 3 Antibody (XVIF9-G10) [NB300-540] - Sodium/Potassium ATPase alpha-3 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) in HeLa cells is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Sodium/Potassium ATPase alpha-3 at a dilution of 1:20 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.

Immunohistochemistry-Paraffin: Sodium Potassium ATPase Alpha 3 Antibody (XVIF9-G10) [NB300-540] - Both normal and cancer biopsies of deparaffinized Human tonsil tissues.

Immunohistochemistry-Paraffin: Sodium Potassium ATPase Alpha 3 Antibody (XVIF9-G10) [NB300-540] - Both normal and cancer biopsies of deparaffinized Human colon tissues.

Simple Western: Sodium Potassium ATPase Alpha 3 Antibody (XVIF9-G10) [NB300-540] - Simple Western lane view shows a specific band for Sodium Potassium ATPase Alpha 3 in 0.5 mg/ml of Human Brain lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

• Reactivity: Hu, Mu, Rt, Bv, Ca, Gp, Pm, Rb, Sh
• Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, B/N, Single-Cell Western
• Clone: XVIF9-G10
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Sodium Potassium ATPase Alpha 3 Antibody (XVIF9-G10) - BSA Free Summary

• Immunogen: Canine cardiac microsomes.
• Marker: Plasma Membrane Marker
• Specificity: Detects sodium / potassium ATPase. This is specific for the alpha-3 subunit.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ATP1A3
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Block/Neutralize
  • Flow Cytometry 1 ug / 10^6 cells
  • Immunocytochemistry/Immunofluorescence 5 ug/mL
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Frozen 3 ug/mL
  • Immunohistochemistry-Paraffin 1:10 - 1:500
  • Simple Western 1:100
  • Single Cell Western 100 ug/mL
  • Western Blot 1 ug/mL
• Application Notes: May be useful in WB, IF, and IHC-Fr. WB: Detects an approx. 110 kDa protein representing the alpha-3 subunit of the sodium/potassium ATPase from canine skeletal muscle extract. IHC: Staining of sodium / potassium ATPase in rat retina yields a pattern consistent with plasma membrane localization.
  
  In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
  Single Cell Western validated using live primary humna cortical neurons.
• Theoretical MW: 111.749 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  Sodium Potassium ATPase Alpha 3 Overexpression Lysate

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 0.02 M potassium phosphate (pH 7.2), 0.15 M NaCl
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Sodium Potassium ATPase Alpha 3 Antibody (XVIF9-G10) - BSA Free

• alpha 3 polypeptide
• ATP1A3
• ATPase, Na+/K+ transporting, alpha 3 polypeptide
• dystonia 12
• DYT12
• EC 3.6.3
• MGC13276
• RDP
• Sodium Potassium ATPase Alpha 3
• Sodium pump subunit alpha-3
• sodium/potassium-transporting ATPase alpha-3 chain
• sodium/potassium-transporting ATPase subunit alpha-3

Background

The sodium/potassium ATPase is an integral membrane enzyme found in all cells of higher organisms and is responsible for the ATP-dependent transport of sodium and potassium across the cell membrane. This membrane-bound enzyme is related to a number of other ATPases including sarcoplasmic and endoplasmic reticulum calcium ATPase (SERCA) and plasma membrane calcium ATPase (PMCA). The sodium/potassium ATPase consists of a large, multipass, transmembrane catalytic subunit, termed the alpha subunit, and an associated smaller glycoprotein, termed the beta subunit. Studies indicate that there are three isoforms of the alpha subunit (alpha 1, alpha 2, alpha 3) and two isoforms of the beta subunit (beta 1 and beta 2) encoded by two multigene families. The different isoforms of the sodium/potassium ATPase exhibit tissue-specific and developmental patterns of expression. The alpha 1 and beta mRNAs are present in all cell types examined, whereas the alpha 2 and alpha 3 mRNAs exhibit a more restricted pattern of cell-specific expression. The alpha-3 subunit has been found in neuronal and to skeletal and cardiac muscle, lung and stomach tissues.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Sodium Potassium ATPase Alpha 3 Antibody (NB300-540)(16)

Publications using NB300-540

• Nordstrom CK, Danckwardt-Lilliestrom N, Liu W, Rask-Andersen H Reversed polarizationof Na/K-ATPase-a sign of inverted transport in the human endolymphatic sac: a super-resolution structured illumination microscopy (SR-SIM) study Cell Tissue Res. 2019-11-12 [PMID: 31713726]
• Coleman LG, Zou J, Crews FT Microglial-derived miRNA let-7 and HMGB1 contribute to ethanol-induced neurotoxicity via TLR7 J Neuroinflammation Jan 25 2017 [PMID: 28118842] (FLOW, ELISA, Human) J Neuroinflammation. 2017-01-25 [PMID: 28118842] (FLOW, Human)
  
  Details:
  Citation using the Alexa Fluor 488 form of this antibody.
• Ivakine EA, Acton BA, Mahadevan V et al. Neto2 is a KCC2 interacting protein required for neuronal Cl- regulation in hippocampal neurons. Proc Natl Acad Sci U S A 2013-02-26 [PMID: 23401525] (WB, Mouse)
• Molday LL, Wu WW, Molday RS. Retinoschisin (RS1), the protein encoded by the X-linked retinoschisis gene, is anchored to the surface of retinal photoreceptor and bipolar cells through its interactions with a Na/K ATPase-SARM1 complex. J Biol Chem;282(45):32792-801. 2007-11-09 [PMID: 17804407] (WB)
• Ferguson SM, Savchenko V, Apparsundaram S, Zwick M, Wright J, Heilman CJ, Yi H, Levey AI, Blakely RD. Vesicular localization and activity-dependent trafficking of presynaptic choline transporters. J Neurosci;23(30):9697-709. 2003-10-29 [PMID: 14585997] (IF/IHC)
• Hoffman JF, Wickrema A, Potapova O, Milanick M, Yingst DR. Na pump isoforms in human erythroid progenitor cells and mature erythrocytes. Proc Natl Acad Sci U S A;99(22):14572-7. 2002-10-29 [PMID: 12388775] (ICC/IF)
• Brickley DR, Mikosz CA, Hagan CR, Conzen SD. Ubiquitin modification of serum and glucocorticoid-induced protein kinase-1 (SGK-1). J Biol Chem;277(45):43064-70. 2002-11-08 [PMID: 12218062] (ICC/IF)
• Kong JQ, Meng J, Biser PS, Fleming WW, Taylor DA. Cellular depolarization of neurons in the locus ceruleus region of the guinea pig associated with the development of tolerance to opioids. J Pharmacol Exp Ther;298(3):909-16. 2001-09-01 [PMID: 11504784] (IF/IHC)
• Wetzel RK, Sweadner KJ. Immunocytochemical localization of NaK-ATPase isoforms in the rat and mouse ocular ciliary epithelium. Invest Ophthalmol Vis Sci. 42(3):763-9. 2001-03-01 [PMID: 11222539] (IF/IHC)
• Tao QF, Hollenberg NK, Graves SW. Sodium pump inhibition and regional expression of sodium pump alpha-isoforms in lens. Hypertension;34(5):1168-74. 1999-11-01 [PMID: 10567200] (WB)
• Halbert D, Domenyuk V, Spetzler D et al. Aptamers and uses thereof United States Patent Application US 9958448 B2 2018-01-01
• Liu W, Luque M, Glueckert R et al. Expression of Na/K-ATPase subunits in the human cochlea: a confocal and super-resolution microscopy study with special reference to auditory nerve excitation and cochlear implantation Ups. J. Med. Sci. 2019-08-01 [PMID: 31460814]
• Ferrer-Ferrer M, Jia S, Kaushik R et al. Disturbance in Hebbian-like spinogenesis blunted learning-induced modulation in the number and spatial distribution of dendritic spines Research Square 2022-06-24 (IF/IHC, Mouse)
• Rose EM, Koo JCP, Antflick JE et al. Glutamate Transporter Coupling to Na,K-ATPase. J Neurosci;29(25):8143-8155. 2009-01-01 [PMID: 19553454]
• Hou Z Matherly LH. Oligomeric Structure of the Human Reduced Folate Carrier: Identification of Homo-Oligomers Dominant-Negative Effects on Carrier Expression Function. J Biol Chem;284(5):3285-3293. 2009-01-01 [PMID: 19019821]
• Pfeiffer R, Beron J, Verrey F. Regulation of Na+ pump function by aldosterone is alpha-subunit isoform specific. J Physiol 1999-04-14 [PMID: 10200415]

Reviews for Sodium Potassium ATPase Alpha 3 Antibody (NB300-540) (2)

Average Rating: 4

(Based on 2 reviews)

We have 2 reviews tested in 2 species: Human, Mouse.

reviewed by: Haiquan Lu WB Human 08/22/2015

Summary

• Application: Western Blot
• Sample Tested: Human cancer cell
• Species: Human

reviewed by: Verified Customer WB Mouse 08/21/2012

Summary

• Application: Western Blot
• Sample Tested: Membrane fraction from whole brain
• Species: Mouse
• Lot: ME 158235

Bioinformatics

• Gene Symbol: ATP1A3
• Entrez:
  • 478(Human)
  • 232975(Mouse)
  • 24213(Rat)
• Uniprot:
  • P13637(Human)