Aquaporin-3 Antibody

Images

 
Western Blot: Aquaporin-3 Antibody [NBP1-97927] - Analysis of Rat kidney inner medullary homogenates showing detection of Aquaporin 3 protein using Rabbit Anti-Aquaporin 3 Polyclonal Antibody. Primary Antibody: Rabbit ...read more
Immunocytochemistry/ Immunofluorescence: Aquaporin-3 Antibody [NBP1-97927] - Tissue: kidney tissue. Species: Rat. Primary Antibody: Rabbit Anti-Aquaporin 3 Polyclonal Antibody at 1:200. Secondary Antibody: FITC Goat ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

Aquaporin-3 Antibody Summary

Immunogen
Produced against the C-terminal peptide (Sequence N-CHLEQPPPSTEAENVKLAHMKHKEQI) of rat aquaporin 3
Localization
Membrane
Specificity
Detects approx 31.5kDa. May detect larger glycosylated bands approx 35-50kDa.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
AQP3
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:4000
  • Immunocytochemistry/Immunofluorescence 1:400
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Paraffin 1:200
Application Notes
0.5 ug/ml of Aquaporin 3 Antibody was sufficient for detection of aquaporin 3 in 10 ug of rat kidney tissue lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary Antibody.
Theoretical MW
32 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using NBP1-97927.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS and 50% Glycerol
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A purified

Alternate Names for Aquaporin-3 Antibody

  • AQP-3
  • aquaglyceroporin-3
  • aquaporin 3 (GIL blood group)
  • aquaporin 3 (Gill blood group)
  • aquaporin 3
  • aquaporin-3
  • GIL
  • Gill blood group

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Aquaporin-3 Antibody (NBP1-97927)(4)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Aquaporin-3 Antibody (NBP1-97927). (Showing 1 - 1 of 1 FAQs).

  1. I am from China and I would like to know more about your product number:NBL1-07638. We know that the theoretical MW of AQP3 protein is 31KD. But in your product specification, we can see that so many bands appear after overexpressing AQP3 with plasmid in 293 cell. Can you explain this phenomenon? Apart from theoretical strip, are all other nonspecific bands? Or is the AQP3 itself ? 
    • Yes, the theoretical size of AQP3 is 31 kDa, which would be expected under reduced conditions with a sample. We have not characterized any of the other bands in the overexpressed lysate lane. Because this is a native lysate, any modifications that may take place could cause smearing and extra bands in the lysate sample. AQP3 is extensively modified because it is a glycoprotein, glycosylation often shows up as multiple bands with smearing when run under native conditions.  This overexpression lysate is meant to only act as a positive control in WB experiments requiring a native control. Furthermore, the lane on the right side of the blot is showing detection of the lysate using an anti-Flag tag antibody, since the construct used for overexpression contains a Flag tag. 

Secondary Antibodies

 

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Blogs on Aquaporin-3

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Bioinformatics

Gene Symbol AQP3
Entrez
Uniprot
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