Human SOD2/Mn-SOD ELISA Kit (Colorimetric)

ELISA: SOD2/Mn-SOD ELISA Kit [HRP] [KA0528] - Standard curve

Product Discontinued

Human SOD2/Mn-SOD ELISA Kit (Colorimetric) Summary

• Description: Quality control test: Standard curve. SOD2 (Human) ELISA Kit is a sandwich enzyme immunoassay for the quantitative measurement of human SOD2.
• Standard Curve Range: 78.125 to 5000.0 pg/mL
• Sensitivity: <5000 pg/mL
• Assay Type: Sandwich ELISA
• Inter-Assay: CV% < 13.7%
• Intra-Assay: CV% < 9.7%
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: SOD2

Applications/Dilutions

• Dilutions:
  • ELISA
  • Quantification
• Application Notes: Useful for quantification.

Packaging, Storage & Formulations

• Storage: Store at 4C.

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Human SOD2/Mn-SOD ELISA Kit (Colorimetric)

• EC 1.15.1.1
• indophenoloxidase B
• IPOB
• IPO-B
• manganese-containing superoxide dismutase
• mangano-superoxide dismutase
• Mn SOD
• Mn superoxide dismutase
• MnSOD
• MVCD6
• SOD, Mitochodrial
• SOD2
• superoxide dismutase [Mn], mitochondrial
• superoxide dismutase 2, mitochondrial

Background

Superoxide dismutase (SOD) is responsible for the elimination of cytotoxic active oxygen by catalyzing the dismutation of the superoxide radical to oxygen and hydrogen peroxide (1, 2, 3). There are three SOD isoenzymes in mammalian cells. They are: extracellular SOD (EC SOD), copper and zinc-containing SOD (CuZn SOD) and manganese-containing SOD (Mn SOD, SOD-2). The CuZn form contains Cu and Zn ions and exists as a 32 kDa dimer in the cytosol. Mn SOD is an 80 kDa tetramer that contains Mn ion and resides in the mitochondrial matrix. Mn SOD is a tumor necrosis factor (TNF)-inducible enzyme that protects cells from NFmediated apoptosis (4) via superoxide anion detoxification and the subsequent regulation of apoptosis through cytochrome c release and the modulation of the redox state of the mitochondria (6). Mn SOD has also been shown to be a tumor suppressor in human breast cancer (7). Overexpression of this enzyme protects neurons from NMDAand nitric oxide-induced neurotoxicity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for SOD2/Mn-SOD ELISA Kit (KA0528)(2)

Publications using KA0528

• Zelko IN, Mariani TJ, Folz RJ. et al. Superoxide dismutase multigene family: a comparison of the CuZn-SOD (SOD1), Mn-SOD (SOD2), and EC-SOD (SOD3) gene structures, evolution, and expression. Free Radic Biol Med 2002-08-01 [PMID: 12126755]
• Macmillan-Crow LA, Cruthirds DL., et al. Invited review: manganese superoxide dismutase in disease. Free Radic Res 2001-04-01 [PMID: 11328670]

FAQs for SOD2/Mn-SOD ELISA Kit (KA0528). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: SOD2