SOCS-6 Overexpression Lysate (Native)


Western Blot: SOCS6 Overexpression Lysate (Adult Normal) [NBL1-16331] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for SOCS6.

Product Details

Applications WB
The exact concentration of the protein of interest cannot be determined for overexpression lysates. Please contact technical support for more information.

Order Details

SOCS-6 Overexpression Lysate (Native) Summary

The lysate was created in HEK293T cells, using plasmid ID RC202224 and based on accession number NM_004232. The protein contains a C-terminal DDK tag.
Homo sapiens suppressor of cytokine signaling 6 (SOCS6), mRNA.

Packaging, Storage & Formulations

Store at -80C. Avoid freeze-thaw cycles.
RIPA buffer


  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
59.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Lysate Details for SOCS-6

Protein State


HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for SOCS-6 Overexpression Lysate (Native)

  • CIS4CIS-4
  • Cytokine-inducible SH2 protein 4
  • HSPC060
  • SOCS4Cish4
  • SOCS6
  • SSI4SOCS-6
  • STAI4
  • STAT induced STAT inhibitor-4
  • STAT4
  • STATI4
  • Suppressor of cytokine signaling 4SOCS-4
  • suppressor of cytokine signaling 6


The protein encoded by this gene contains a SH2 domain and a CIS homolog domain. The protein thus belongs to the cytokine-induced STAT inhibitor (CIS), also known as suppressor of cytokine signaling (SOCS) or STAT-induced STAT inhibitor (SSI), protein family. CIS family members are known to be cytokine-inducible negative regulators of cytokine signaling. The expression of this gene can be induced by GM-CSF and EPO in hematopoietic cells. A high expression level of this gene was found in factor-independent chronic myelogenous leukemia (CML) and erythroleukemia (HEL) cell lines. [provided by RefSeq].


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Video Protocols

WB Video Protocol

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Contact Information

Product PDFs


Gene Symbol SOCS6

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