SOCS-6 Overexpression Lysate (Native)

Images

 
Western Blot: SOCS6 Overexpression Lysate (Adult Normal) [NBL1-16331] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for SOCS6.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

SOCS-6 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC202224 and based on accession number NM_004232. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens suppressor of cytokine signaling 6 (SOCS6), mRNA.
Gene
SOCS6

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
59.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for SOCS-6

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for SOCS-6 Overexpression Lysate (Native)

  • CIS4CIS-4
  • Cytokine-inducible SH2 protein 4
  • HSPC060
  • SOCS4Cish4
  • SOCS6
  • SOCS-6
  • SSI4SOCS-6
  • STAI4
  • STAT induced STAT inhibitor-4
  • STAT4
  • STATI4
  • Suppressor of cytokine signaling 4SOCS-4
  • suppressor of cytokine signaling 6

Background

The protein encoded by this gene contains a SH2 domain and a CIS homolog domain. The protein thus belongs to the cytokine-induced STAT inhibitor (CIS), also known as suppressor of cytokine signaling (SOCS) or STAT-induced STAT inhibitor (SSI), protein family. CIS family members are known to be cytokine-inducible negative regulators of cytokine signaling. The expression of this gene can be induced by GM-CSF and EPO in hematopoietic cells. A high expression level of this gene was found in factor-independent chronic myelogenous leukemia (CML) and erythroleukemia (HEL) cell lines. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP1-30943
Species: Hu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-27210
Species: Hu, Fe, Pm
Applications: WB, IHC-P
NB100-56637
Species: Hu, Mu
Applications: WB
AF4979
Species: Hu
Applications: WB
NB100-56639
Species: Hu, Mu
Applications: WB
MAB5809
Species: Hu
Applications: WB
AF2988
Species: Mu, Rt
Applications: WB
NBP2-22203
Species: Hu, Mu, Rt, Mk
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
AF231
Species: Hu
Applications: WB, Flow, IHC, IP, CyTOF-ready, ELISA(Cap), ELISA(Det), ICC, ELISA(Sta)
AF1584
Species: Hu, Mu
Applications: WB, Simple Western, IP, CyTOF-ready, ICC, ICFlow
NBP2-34260
Species: Hu, Mu, Po, Bv, Rb
Applications: WB, Flow, ICC/IF, IHC-Fr, IHC-P, IP, PAGE
NBP2-00622
Species: Hu, Rt, Ca, Pm
Applications: WB, Flow, ICC/IF, IHC, IHC-P
NB100-56749
Species: Hu, Mu
Applications: WB, IHC, IHC-P
NBP2-22471
Species: Hu, Mu, Rt, Mk, Pm
Applications: WB, ChIP, ICC/IF, IHC, IHC-P, IP
NBP2-16372
Species: Hu
Applications: WB, IHC-P
NBL1-16331
Species: Hu
Applications: WB

Publications for SOCS-6 Lysate (NBL1-16331) (0)

There are no publications for SOCS-6 Lysate (NBL1-16331).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for SOCS-6 Lysate (NBL1-16331) (0)

There are no reviews for SOCS-6 Lysate (NBL1-16331). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for SOCS-6 Lysate (NBL1-16331) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional SOCS-6 Products

Bioinformatics Tool for SOCS-6 Lysate (NBL1-16331)

Discover related pathways, diseases and genes to SOCS-6 Lysate (NBL1-16331). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for SOCS-6 Lysate (NBL1-16331)

Discover more about diseases related to SOCS-6 Lysate (NBL1-16331).
 

Pathways for SOCS-6 Lysate (NBL1-16331)

View related products by pathway.

PTMs for SOCS-6 Lysate (NBL1-16331)

Learn more about PTMs related to SOCS-6 Lysate (NBL1-16331).
 

Research Areas for SOCS-6 Lysate (NBL1-16331)

Find related products by research area.

Blogs on SOCS-6

There are no specific blogs for SOCS-6, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our SOCS-6 Overexpression Lysate (Native) and receive a gift card or discount.

Bioinformatics

Gene Symbol SOCS6
Entrez