SOCS-4 Antibody

Immunohistochemistry-Paraffin: SOCS-4 Antibody [NB100-898] - 3.8ug/ml staining of paraffin embedded Human Pancreas. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu, Po, Bv
• Applications: ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

SOCS-4 Antibody Summary

• Immunogen: Peptide with sequence C-SKVRVLRIDAPEQQ corresponding to C-Terminus according to NP_543143.1, NP_955453.1.
• Specificity: Both variants represent identical protein (NP_543143.1 and NP_955453.1).
• Predicted Species: Porcine (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: SOCS4
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 3 - 6 ug/ml
  • Immunohistochemistry-Paraffin 3 - 6 ug/ml
  • Peptide ELISA Detection limit 1:32000
• Application Notes: WB: Preliminary experiments in human and Rodent brain lysates and in lysates of NIH3T3, A431 and HeLa gave no specific signal but low background. IHC-P: Human pancreas shows cytoplasm staining on select beta cells in islet of langerhans.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for SOCS-4 Antibody

• CIS4
• DKFZp686J1568
• SOCS4
• SOCS-4
• SOCS7
• SOCS-7
• SSI4
• STAT4
• suppressor of cytokine signaling 4
• Suppressor of cytokine signaling 7SH2 domain containing SOCS box protein

Background

The eight members of the recently identified Suppressor of Cytokines Signaling (SOCS) family are SOCS1, SOCS2, SOCS3, SOCS4, SOCS5, SOCS6, SOCS7, and CIS. Structurally the SOCS proteins are composed of an N- terminal region of variable length and amino acid composition, a central SH2 domain, and a C-terminal motif called the SOCS box. The SOCS proteins appear to form part of a classical negative feedback loop that regulates cytokine signal transduction. Transcription of each of the SOCS genes occurs rapidly in vitro and in vivo in response to cytokines, and once produced, the various members of the SOCS family appear to inhibit signaling in different ways. SOCS1 and SOCS6 interact with the insulin receptor (IR) when expressed in human hepatoma cells (HepG2) or in rat hepatoma cells overexpressing human IR. SOCS1 and SOCS6 inhibit insulin-dependent activation of ERK1/2 and protein kinase B in vivo and IR- directed phosphorylation of IRS1 in vitro. These results suggest that SOCS proteins may be inhibitors of IR signalling and could mediate cytokine-induced insulin resistance and contribute to the pathogenesis of type II diabetes. SOCS6 and SOCS7 are expressed ubiquitously in murine tissues and SOCS6 knockout mice are growth retarded.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SOCS-4 Antibody (NB100-898)(1)

Publication using NB100-898

• Kario E, Marmor MD, Adamsky K et al. Suppressors of cytokine signaling 4 and 5 regulate epidermal growth factorreceptor signaling. J Biol Chem 2005-02-25 [PMID: 15590694]

Bioinformatics

• Gene Symbol: SOCS4
• Entrez:
  • 122809(Human)
• Uniprot:
  • NP_543143.(Human)