SOCS-3 Antibody [Janelia Fluor® 549]

• Reactivity: Hu, Fe, Pm, Po, Pm, Pm-Or, RM
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Janelia Fluor 549

SOCS-3 Antibody [Janelia Fluor® 549] Summary

• Immunogen: A portion of amino acids 110-160 from human SOCS3 was used as the immunogen for this antibody.
• Predicted Species: Porcine (94%), Chimpanzee (100%), Orangutan (100%), Rhesus Macaque (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SOCS3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

The amino acid sequence used as immunogen for this antibody is 100% homologous in rhesus monkey, orangutan, european pole-cat, and chimpanzee; 94% in new world monkey and pig; 89% in panda, dog, chinese hamster, and horse; 83% in cow, african bush elephant, mouse, sheep, and rat; and 61% homologous in guinea pig.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Immunogen affinity purified

Notes

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for SOCS-3 Antibody [Janelia Fluor® 549]

• ATOD4
• CIS3
• CIS3CIS-3
• Cish3
• cytokine-induced SH2 protein 3
• Cytokine-inducible SH2 protein 3
• SOCS3
• SOCS-3
• SOCS-3STAT-induced STAT inhibitor 3
• SSI-3
• SSI-3ATOD4
• SSI3MGC71791
• suppressor of cytokine signaling 3

Background

Accumulating evidence has demonstrated that cytokine receptor signaling is negatively regulated by a family of Src homology 2 domain-containing adaptor molecules termed SOCS (Suppressor of Cytokine Signaling) (1-3). To date, there are eight members of SOCS family that have been recognized, they are SOCS-1, 2, 3, 4, 5, 6, 7 and CIS. Structurally, the SOCS proteins are composed of an N-terminal region of variable length and amino acid composition, a central SH2 domain, and a previously unrecognized C-terminal motif that has been called the SOCS box (4). The SOCS proteins appear to form part of a classical negative feed back loop that regulates cytokine signal transduction via a STAT-induced transcriptional mechanism (5). Transcription of each of the SOCS genes occurs rapidly in vitro and in vivo in response to cytokines, and once produced, the various members of the SOCS family appear to inhibit signaling in different ways. SOCS 3 is an important regulator of fetal liver hematopoiesis. It is also involved in a broad spectrum of cytokines, e.g. IL-2, IL-3, IL-4, IL-6, Epo, Prolactin, and GH (6-8).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SOCS3