SOCS-3 Antibody (SO1) - BSA Free

Western Blot: SOCS3 Antibody (SO1) [NB600-1075] - Fig. 1. Western Blotting analysis (reducing conditions) of whole cell lysate of HeLa human cervix carcinoma cell line. Lane 1: immunostaining with Isotype mouse IgG2b control (PLRV219) Lane 2: immunostaining with anti-SOCS3 (SO1).

• Reactivity: Hu, Rt
• Applications: WB
• Clone: SO1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

SOCS-3 Antibody (SO1) - BSA Free Summary

• Description: Novus Biologicals Mouse SOCS-3 Antibody (SO1) - BSA Free (NB600-1075) is a monoclonal antibody validated for use in WB. Anti-SOCS-3 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full-length SOCS3 protein.
• Specificity: The antibody SO1 reacts with SOCS3, an intracellular cytokine signaling inhibitor.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: SOCS3
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:1000
• Application Notes: Western Blot - Sample preparation: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with reducing Laemmli SDS-PAGE sample buffer. Application note: Reducing conditions.

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 24142708).

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Phosphate buffered saline (PBS), pH 7.4
• Preservative: 15mM Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein A purified

Alternate Names for SOCS-3 Antibody (SO1) - BSA Free

• ATOD4
• CIS3
• CIS3CIS-3
• Cish3
• cytokine-induced SH2 protein 3
• Cytokine-inducible SH2 protein 3
• SOCS3
• SOCS-3
• SOCS-3STAT-induced STAT inhibitor 3
• SSI-3
• SSI-3ATOD4
• SSI3MGC71791
• suppressor of cytokine signaling 3

Background

SOCS3 (suppressor of cytokine signaling 3), also known as CIS3 (cytokine-inducible SH2 protein 3) is a negative regulator of particular cytokine signaling pathways. SOCS3 is induced by a variety of cytokines and other stimuli, such as erythropoietin, leptin and lipopolysaccharides and inhibits tyrosinkinase activity of JAK kinases, or e.g. JNK phosphorylation. SOCS3 modulates cytokine-mediated and neoplastic-proliferative responses and is involved also in maintaining leukocytes in quiescent state until antigen stimulation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SOCS-3 Antibody (NB600-1075)(1)

Publication using NB600-1075

• Xiang S, Dong NG, Liu JP et al. Inhibitory effects of suppressor of cytokine signaling 3 on inflammatory cytokine expression and migration and proliferation of IL-6/IFN-gamma-induced vascular smooth muscle cells. J Huazhong Univ Sci Technolog Med Sci. 2013-10-01 [PMID: 24142708] (WB, Rat)

Bioinformatics

• Gene Symbol: SOCS3
• Entrez:
  • 89829(Rat)