SNIP1 Antibody

Product Discontinued

SNIP1 Antibody Summary

• Immunogen: A synthetic peptide derived from a sequence between residues 225 and 275 of human Smad nuclear interacting protein 1 using the numbering given in Swiss-Prot entry Q8TAD8 (GeneID 79753).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SNIP1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 1-4 ug/mg lysate
  • Western Blot 1:1000-1:10000
• Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Prepare working dilution immediately before use. Suggested working dilutions: * Western Blot: 1:1,000 - 1:10,000 Immunoprecipitation: 1 to 4 ug/mg lysate Histo/Cytochemistry: not tested *The investigator should determine the optimal working dilution for a specific application.
• Control:

  SNIP1 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for SNIP1 Antibody

• dJ423B22.2
• FHA domain-containing protein SNIP1
• FLJ12553
• RP3-423B22.3
• Smad nuclear interacting protein (SNIP1)
• Smad nuclear interacting protein 1
• smad nuclear-interacting protein 1

Background

Members of the transforming growth factor-beta superfamily play critical roles in controlling cell growth and differentiation. Effects of TGF-beta family ligands are mediated by Smad proteins. A 396-amino acid nuclear protein termed SNIP1 was cloned and shown to harbor a nuclear localization signal (NLS) and a Forkhead-associated (FHA) domain. The carboxyl terminus of SNIP1 interacts with Smad1 and Smad2 in yeast two-hybrid as well as in mammalian overexpression systems. However, the amino terminus of SNIP1 harbors binding sites for both Smad4 and the coactivator CBP/p300. Overexpression of full-length SNIP1 or its amino terminus is sufficient to inhibit multiple gene responses to TGF-beta and CBP/p300, as well as the formation of a Smad4/p300 complex. Studies in Xenopus laevis further suggest that SNIP1 plays a role in regulating dorsomedial mesoderm formation by the TGF-beta family member nodal. Thus, SNIP1 is a nuclear inhibitor of CBP/p300 and its level of expression in specific cell types has important physiological consequences by setting a threshold for TGF-beta-induced transcriptional activation involving CBP/p300.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SNIP1 Antibody (NB100-512)(1)

Publication using NB100-512

• Yu B, Bi L, Zheng B et al. The FHA domain proteins DAWDLE in Arabidopsis and SNIP1 in humans act in small RNA biogenesis. Proc Natl Acad Sci U S A 2008-07-01 [PMID: 18632581]

Bioinformatics

• Gene Symbol: SNIP1
• Entrez:
  • 79753(Human)
• Uniprot:
  • Q8TAD8(Human)