SNF8 Antibody (6B11) - Azide and BSA Free

Western Blot: SNF8 Antibody (6B11) [H00011267-M01] - Analysis of SNF8 expression in transfected 293T cell line by SNF8 monoclonal antibody (M01), clone 6B11.Lane 1: SNF8 transfected lysate (Predicted MW: 28.9 KDa).Lane 2: Non-transfected lysate.

Western Blot: SNF8 Antibody (6B11) [H00011267-M01] - EAP30 monoclonal antibody (M01), clone 6B11 Western Blot analysis of SNF8 expression in HeLa NE ( Cat # L013V3 ).

Sandwich ELISA: SNF8 Antibody (6B11) [H00011267-M01] - Detection limit for recombinant GST tagged SNF8 is approximately 1ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA
• Clone: 6B11
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

SNF8 Antibody (6B11) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse SNF8 Antibody (6B11) - Azide and BSA Free (H00011267-M01) is a monoclonal antibody validated for use in WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: SNF8 (NP_009172, 23 a.a. ~ 122 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. ERGTVLAEDQLAQMSKQLDMFKTNLEEFASKHKQEIRKNPEFRVQFQDMCATIGVDPLASGKGFWSEMLGVGDFYYELGVQIIEVCLALKHRNGGLITLE
• Specificity: SNF8 - EAP30 subunit of ELL complex
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: SNF8
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactive against cell lysate and recombinant protein for western blot. It has also been used for ELISA.

Reactivity Notes

Human. Other species not tested.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for SNF8 Antibody (6B11) - Azide and BSA Free

• EAP30 subunit of ELL complex
• EAP30hVps22
• ELL-associated protein of 30 kDa
• ESCRT-II complex subunit VPS22
• SNF8, ESCRT-II complex subunit, homolog (S. cerevisiae)
• vacuolar-sorting protein SNF8
• VPS22Dot3

Background

SNF8, VPS25 (MIM 610907), and VPS36 (MIM 610903) form ESCRT-II (endosomal sorting complex required for transport II), a complex involved in endocytosis of ubiquitinated membrane proteins. SNF8, VPS25, and VPS36 are also associated in a multiprotein complex with RNA polymerase II elongation factor (ELL; MIM 600284) (Slagsvold et al., 2005 [PubMed 15755741]; Kamura et al., 2001 [PubMed 11278625]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SNF8
• Entrez:
  • 11267(Human)
• Uniprot:
  • NP_009172(Human)