SNF5 Antibody (CL13992) - Azide and BSA Free

Staining of human breast cancer (ductal carcinoma) shows strong nuclear positivity in tumor cells.

Staining of human cerebral cortex shows strong nuclear positivity in neurons.

Staining of human fallopian tube shows strong nuclear positivity in glandular cells.

Staining of human rectum shows strong nuclear positivity in glandular cells and lymphoid cells.

Staining of human ovarian cancer 8papillary carcinoma) shows strong nuclear positivity in tumor cells.

Staining of human prostate shows moderate nuclear positivity in glandular cells.

Staining of human ovarian cancer (high grade serous ovarian carcinoma) shows moderate to strong nuclear positivity in tumor cells.

Analysis in human cell line SK-MEL-30.

• Reactivity: Hu
• Applications: WB, IHC
• Clone: CL13992
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

SNF5 Antibody (CL13992) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse SNF5 Antibody (CL13992) - Azide and BSA Free (NBP3-24593) is a monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was generated using a recombinant protein sequence of Q12824, with the exact immunogen sequence remaining proprietary.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: SMARCB1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 1 ug/ml
• Application Notes: For IHC-Paraffin, we recommend using Heat-Induced Epitope Retrieval (HIER) with a pH level of 6. This optimized retrieval method ensures the best results for your experiments.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered solution in PBS with Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A purified
• Reconstitution Instructions: Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Alternate Names for SNF5 Antibody (CL13992) - Azide and BSA Free

• BAF47
• BAF47hSNF5
• BRG1-associated factor 47
• hSNFS
• Ini1
• INI1RTPS1
• Integrase interactor 1 protein
• malignant rhabdoid tumor suppressor
• RDTSNF5 homolog
• Sfh1p
• SNF5
• SNF5L1
• Snr1
• subfamily b, member 1
• sucrose nonfermenting, yeast, homolog-like 1
• SWI/SNF related, matrix associated, actin dependent regulator of chromatin
• SWI/SNF-related matrix-associated actin-dependent regulator of chromatinsubfamily B member 1

Background

SNF5 is encoded by this gene is part of a complex that relieves repressive chromatin structures, allowing the transcriptional machinery to access its targets more effectively. The encoded nuclear protein may also bind to and enhance the DNA joining activity of HIV-1 integrase. This gene has been found to be a tumor suppressor, and mutations in it have been associated with malignant rhabdoid tumors. Two transcript variants encoding different isoforms have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SMARCB1