SNAP25 Antibody [Alexa Fluor® 405]

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor 405

SNAP25 Antibody [Alexa Fluor® 405] Summary

• Immunogen: E. coli-derived recombinant human SNAP25
  Asp140-Gly206
  Accession # P60880
• Specificity: Detects human, mouse, and rat SNAP25 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross‑reactivity with recombinant human SNAP23 is observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for SNAP25 Antibody [Alexa Fluor® 405]

• bA416N4.2
• dJ1068F16.2
• FLJ23079
• resistance to inhibitors of cholinesterase 4 homolog
• RIC4
• RIC-4
• SEC9
• SNAP25
• SNAP-25SNAP
• SUP
• Super protein
• Synaptosomal-associated 25 kDa protein
• synaptosomal-associated protein 25
• synaptosomal-associated protein, 25kD
• synaptosomal-associated protein, 25kDa

Background

SNAP25 (Synaptosomal associated protein of 25 kDa; also SUP) is a cytosolic 24-29 kDa member of the SNAP25 family of proteins. It is expressed in neurons, beta ‑cells, and adrenal chromaffin cells. SNAP25 is involved in vesicle exocytosis, where two presynaptic SNAP25, and one syntaxin‑1 molecule associates with vesicle membrane VAMP2 to form a alpha ‑helix via a zipper‑like mechanism. This approximates two membranes, which subsequently fuse to create a pore. Human SNAP25b is 206 amino acids (aa) in length. It contains two t‑SNARE coiled‑coil homology domains (aa 19‑81 and 140‑202) and a proteolytic cleavage site at Arg180:Ile181. Palmitoylation occurs between aa 85‑92; phosphorylation occurs on Thr138. There are three isoform variants. One shows nine aa changes over aa 58‑89 (isoform a), a second shows a 30 aa substitution for aa 1‑94, while a third contains an alternative start site at Met64. Full‑length human and mouse SNAP25 are identical in amino acid sequence.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.