SNAP23 Antibody

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Western Blot: SNAP23 Antibody [NBP1-52065] - (0.2ug/ml) Analysis of PBMC lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated

Order Details

SNAP23 Antibody Summary

Immunogen
Peptide with sequence C-TDKADTNRDR corresponding to C-Terminus according to NP_570710.1.
Epitope
C-TDKADTNRDR
Specificity
This antibody is expected to recognize both isoforms (NP_003816.2; NP_570710.1). Amino acid numbering in name refers to NP_003816.2 sequence.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
SNAP23
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.2 - 0.6 ug/ml
  • Peptide ELISA Detection limit 1:2000
Application Notes
WB: Approx. 20+26 kDa band bands observed in human peripheral blood mononucleocytes lysates (calculated MW of 17.8 kDa band according to NP_570710.1 and of 23.4 kDa band according to NP_003816.2).
Publications
Read Publication using NBP1-52065.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Mouse, Rat, Canine, Bovine, Rabbit.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for SNAP23 Antibody

  • HsT17016
  • SNAP23
  • SNAP-23
  • SNAP23A
  • SNAP23B
  • synaptosomal-associated protein 23
  • synaptosomal-associated protein, 23kD
  • synaptosomal-associated protein, 23kDa
  • Vesicle-membrane fusion protein SNAP-23

Background

Synaptosomal-associated proteins (SNAPs) are cytosolic proteins that play a key role in in the process of membrane fusion in intracellular vesicle trafficking. In eukaryotic cells, the SNARE (soluble N-ethylmaleimide-sensitive factor attachment protein receptor) complex is critical to membrane docking and fusion and is believed to impart some degree of specificity between vesicle SNARE (v-SNARE) and target organelle SNARE (t-SNARE). In neurons and neuroendocrine cells, the SNARE complex consists of the integral membrane proteins VAMP (vesicle-associated membrane protein), syntaxin and SNAP-25. In non-neuronal tissue, a SNAP-25 homolog, SNAP-23, functionally replaces SNAP-25 in the SNARE complex. Studies show that VAMP, syntaxin and SNAP-23 are required for SNARE function and that this complex exists as a heterotrimer of the three proteins. In insulin signalling pathways, studies suggest that the translocation of GLUT4 in adipocytes requires that functional SNAP-23 be present in SNARE complexes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for SNAP23 Antibody (NBP1-52065)(1)

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Video Protocols

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol SNAP23
Entrez
Uniprot