Snail Overexpression Lysate (Native)

Images

 
Western Blot: SNAIL Overexpression Lysate (Adult Normal) [NBL1-16265] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for SNAIL.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

Snail Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC204581 and based on accession number NM_005985. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens snail homolog 1 (Drosophila) (SNAI1), mRNA.
Gene
SNAI1

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
28.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Snail

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for Snail Overexpression Lysate (Native)

  • Protein sna
  • Protein snail homolog 1
  • SLUGH2
  • SNA
  • SNAH
  • SNAHdJ710H13.1
  • SNAI1
  • snail 1 (drosophila homolog), zinc finger protein
  • snail 1 homolog
  • snail 1 zinc finger protein
  • snail 1, zinc finger protein
  • snail homolog 1 (Drosophila)
  • Snail
  • zinc finger protein SNAI1

Background

The Drosophila embryonic protein snail is a zinc finger transcriptional repressor which downregulates the expression of ectodermal genes within the mesoderm. The nuclear protein encoded by this gene is structurally similar to the Drosophila snail protein, and is also thought to be critical for mesoderm formation in the developing embryo. At least two variants of a similar processed pseudogene have been found on chromosome 2. [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for Snail Lysate (NBL1-16265) (0)

There are no publications for Snail Lysate (NBL1-16265).
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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for Snail Lysate (NBL1-16265). (Showing 1 - 1 of 1 FAQ).

  1. There are many kinds of antibodies you supply against SNAIL. Could you give me your recommendation? Which is the best for the IHC-P experiments the species of my samples to be tested is human.
    • Our SNAIL antibody with catalog # NBP1-19529 has been successfully validated for IHC-P in paraffin-embedded human lung carcinoma tissue and I would highly recommend you to use the same for your samples too. The working dilutions of this antibody for IHC-P ranges from 1:50 - 1:200 and beside IHC, you can use this antibody for Western Blot and Immunofluorescence also.

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Bioinformatics

Gene Symbol SNAI1
Entrez