SMURF1 Overexpression Lysate (Native)


Western Blot: SMURF1 Overexpression Lysate (Adult Normal) [NBP2-09895] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for SMURF1.

Product Details

Reactivity HuSpecies Glossary
Applications WB

Order Details

SMURF1 Overexpression Lysate (Native) Summary

The lysate was created in HEK293T cells, using plasmid ID RC215316 and based on accession number NP_065162. The protein contains a C-terminal DDK tag.
Homo sapiens SMURF1, mRNA.


Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
85.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Store at -80C. Avoid freeze-thaw cycles.
RIPA buffer

Lysate Details for SMURF1

Protein State


HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for SMURF1 Overexpression Lysate (Native)

  • E3 ubiquitin ligase SMURF1
  • EC 6.3.2
  • EC 6.3.2.-
  • hSMURF1
  • KIAA1625
  • KIAA1625E3 ubiquitin-protein ligase SMURF1
  • SMAD specific E3 ubiquitin protein ligase 1
  • SMAD ubiquitination regulatory factor 1
  • Smad-specific E3 ubiquitin ligase 1
  • SMAD-specific E3 ubiquitin-protein ligase 1
  • SMURF1


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Video Protocols

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Gene Symbol SMURF1