SMPD3/NSMase2 Antibody

Western Blot: SMPD3/NSMase2 Antibody [NBP3-23413] - Western blot of adult mouse brain. The blots were probed with SMPD3/NSMase2 (C-terminal region) rabbit polyclonal antibody at 1:2000 (lane 1), 1:1000 (lane 2), or 1:500 in the absence (lane 3) or presence of nSMase2 blocking peptide (lane 4).

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

SMPD3/NSMase2 Antibody Summary

• Description: Stable for at least 1 year at -20°C.
• Immunogen: SMPD3/NSMase2 synthetic peptide corresponds to amino acids in the C-terminal region of human SMPD3/NSMase2. This sequence is highly conserved in rat and mouse SMPD3/NSMase2, and has low homology to other nSMase family members. [UniProt# Q9NY59]
• Specificity: The antibody detects a 78 kDa band corresponding to SMPD3/NSMase2 on SDS-PAGE immunoblots of adult mouse brain and rat PC12 cells.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SMPD3
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Western Blot 1:1000
• Theoretical MW: 78 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C.
• Buffer: PBS, 1 mg/ml BSA and 50% glycerol
• Preservative: 0.05% Sodium Azide
• Purity: Antigen Affinity-purified

Alternate Names for SMPD3/NSMase2 Antibody

• EC 3.1.4.12
• FLJ22593
• MGC138443
• Neutral sphingomyelinase 2
• Neutral sphingomyelinase II
• NSMASE2
• nSMase-2
• SMPD3
• sphingomyelin phosphodiesterase 3
• sphingomyelin phosphodiesterase 3, neutral membrane (neutral sphingomyelinaseII)

Background

NSMase2 catalyzes the initial reaction in O-linked oligosaccharide biosynthesis, the transfer of an N-acetyl-D-galactosamine residue to a serine or threonine residue on the protein receptor. Has activity toward EA2 peptide substrate, but has a weak activity towar

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SMPD3