SMN Antibody (5H1)

Western Blot: SMN Antibody (5H1) [NBP2-37594] - Western blot analysis using SMN1 mouse mAb against RAJI (1), Cos7 (2), Jurkat (3), K562 (4), Hela (5) and HepG2 (6) cell lysate.

Immunocytochemistry/Immunofluorescence: SMN Antibody (5H1) [NBP2-37594] - Immunofluorescence analysis of HepG2 cells using SMN1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Immunohistochemistry: SMN Antibody (5H1) [NBP2-37594] - Immunohistochemical analysis of paraffin-embedded stomach cancer tissues (left) and brain tumor (right) using SMN1 mouse mAb with DAB staining.

Immunohistochemistry: SMN Antibody (5H1) [NBP2-37594] - Immunohistochemical analysis of paraffin-embedded breast cancer tissues (left) and testis tissues (right) using SMN1 mouse mAb with DAB staining.

ELISA: SMN Antibody (5H1) [NBP2-37594] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Product Discontinued

SMN Antibody (5H1) Summary

• Immunogen: Purified recombinant fragment of human SMN1 expressed in E. Coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: SMN1
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Immunocytochemistry/ Immunofluorescence 1:200 - 1:1000
  • Immunohistochemistry 1:200 - 1:1000
  • Immunohistochemistry-Paraffin 1:200 - 1:1000
  • Western Blot 1:500 - 1:2000
• Theoretical MW: 39 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Ascites
• Preservative: 0.03% Sodium Azide
• Purity: Unpurified

Alternate Names for SMN Antibody (5H1)

• Gemin-1
• Kugelberg-Welander disease)
• SMNC
• survival motor neuron protein
• survival of motor neuron 1, telomeric

Background

SMN (Gemin 1) is the central component of a large oligomeric complex (SMN complex), including Gemins 2-7, that is necessary and sufficient for the in vivo assembly of Sm proteins onto the small nuclear (sn)RNAs that mediate pre-mRNA splicing. The SMN complex is found in the cytoplasm and nucleus. Within the nucleus, this protein localizes to subnuclear bodies called gems which are found near coiled bodies containing high concentrations of small ribonucleoproteins (snRNPs). Spinal muscular atrophy, a motor neuron disorder, results from reduced levels or a mutation in the Gemin 1 protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for SMN Antibody (NBP2-37594). (Showing 1 - 1 of 1 FAQ).

1. Could you recommond the Human specific SMN antibody? It will only recognize human SMN, without cross reaction with mouse smn.
   • We do not have an SMN antibody that has been validated NOT to work in mouse samples. When an antibody is found to not react with a certain species, we mark the data sheet with a (-) next to the species' name. Unfortunately, the SMN antibodies that do not list mouse have not been tested in mouse, and may react.

Bioinformatics

• Gene Symbol: SMN1