SMC3 Antibody

Western Blot: SMC3 Antibody [NBP1-19109] - Whole cell lysate from HeLa (5, 15 and 50 ug), 293T (T; 50 ug) and mouse NIH3T3 (M; 50ug) cells. Antibody used at for WB at 0.04 ug/ml.

• Reactivity: Hu, Mu
• Applications: WB, IP (-)
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

SMC3 Antibody Summary

• Description: Novus Biologicals Rabbit SMC3 Antibody (NBP1-19109) is a polyclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen recognized by this antibody maps to a region between residue 325 and 375 of human structural maintenance of chromosome 3 using the numbering given in entry NP_005436.1 (GeneID 9126).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SMC3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:2000-1:10000
• Application Notes: This antibody is useful for western blot. It is not recommended for immunoprecipitation.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for SMC3 Antibody

• Bamacan
• BAMSMC protein 3
• Basement membrane-associated chondroitin proteoglycan
• BMH
• chondroitin sulfate proteoglycan 6 (bamacan)
• Chondroitin sulfate proteoglycan 6
• Chromosome-associated polypeptide
• CSPG6structural maintenance of chromosomes protein 3
• hCAP
• HCAPbamacan
• SMC-3
• SMC3L1CDLS3
• structural maintenance of chromosomes 3

Background

This gene belongs to the SMC3 subfamily of SMC proteins. The encoded protein occurs in certain cell types as either an intracellular, nuclear protein or a secreted protein. The nuclear form, known as structural maintenance of chromosomes 3, is a component of the multimeric cohesin complex that holds together sister chromatids during mitosis, enabling proper chromosome segregation. Post-translational modification of the encoded protein by the addition of chondroitin sulfate chains gives rise to the secreted proteoglycan bamacan, an abundant basement membrane protein. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SMC3
• Entrez:
  • 9126(Human)
  • 13006(Mouse)
• Uniprot:
  • NP_005436.1(Human)