SMC3 Antibodies
We offer SMC3 Antibodies for use in common research applications: ELISA, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Immunoprecipitation (Negative), Proximity Ligation Assay, Sandwich ELISA, Western Blot. Each SMC3 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our SMC3 Antibodies can be used in a variety of model species: Drosophila, Human, Mouse, Rat, Xenopus. Use the list below to choose the SMC3 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our SMC3 polyclonal antibodies and browse our SMC3 monoclonal antibody catalog.
Alternate Names for SMC3 Antibodies
anti-SMC3 antibody, anti-Bamacan antibody, anti-BAMSMC protein 3 antibody, anti-Basement membrane-associated chondroitin proteoglycan antibody, anti-BMH antibody, anti-chondroitin sulfate proteoglycan 6 (bamacan) antibody, anti-Chondroitin sulfate proteoglycan 6 antibody, anti-Chromosome-associated polypeptide antibody, anti-CSPG6structural maintenance of chromosomes protein 3 antibody, anti-hCAP antibody, anti-HCAPbamacan antibody, anti-SMC-3 antibody, anti-SMC3L1CDLS3 antibody, anti-structural maintenance of chromosomes 3 antibody
13 Results for "smc3" in Primary Antibodies
![Western Blot: SMC3 Antibody [NB100-207] - Whole cell lysate (10 ug) from HEK293T, HeLa, Jurkat, LNCaP, U2OS, A-549, TCMK-1, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinitypurified rabbit anti-SMC3 antibody used for WB at 0.04 ug/ml. Detection:Chemiluminescence with an exposure time of 3 seconds.](http://images.novusbio.com/fullsize/SMC3-Antibody-Western-Blot-NB100-207-img0026.jpg "Western Blot: SMC3 Antibody [NB100-207] - Whole cell lysate (10 ug) from HEK293T, HeLa, Jurkat, LNCaP, U2OS, A-549, TCMK-1, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinitypurified rabbit anti-SMC3 antibody used for WB at 0.04 ug/ml. Detection:Chemiluminescence with an exposure time of 3 seconds.")
![Immunohistochemistry-Paraffin: SMC3 Antibody [NB100-207] - Section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-SMC3 used at 1:5,000 (0.2ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/SMC3-Antibody-Immunohistochemistry-Paraffin-NB100-207-img0028.jpg)
![Immunohistochemistry-Paraffin: SMC3 Antibody [NB100-207] - Section of human breast carcinoma. Antibody: Affinity purified rabbit anti-SMC3 used at 1:5,000 (0.2ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/SMC3-Antibody-Immunohistochemistry-Paraffin-NB100-207-img0027.jpg)
![SMC3 Antibody [NB100-207] - Detection of human SMC3 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa, cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SMC3 antibody NB100-207(lot NB100-207-9) used for IP at 3 ug per reaction. SMC3 was also immunoprecipitated by a previous lot of this antibody (lot NB100-207-8). For blotting immunoprecipitated SMC3, NB100-207 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/SMC3-Antibody-NB100-207-img0025.jpg)
![Western Blot: SMC3 Antibody [NBP2-20418] - Sample (30 ug of whole cell lysate) A: Hep G2 5% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/SMC3-Antibody-Western-Blot-NBP2-20418-img0003.jpg "Western Blot: SMC3 Antibody [NBP2-20418] - Sample (30 ug of whole cell lysate) A: Hep G2 5% SDS PAGE gel, diluted at 1:1000.")
![Immunocytochemistry/Immunofluorescence: SMC3 Antibody [NBP2-20418] - Analysis of paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/SMC3-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-20418-img0005.jpg)
![Immunohistochemistry-Paraffin: SMC3 Antibody [NBP2-20418] - Immunohistochemical analysis of paraffin-embedded Cal27 Xenograft, using antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/SMC3-Antibody-Immunohistochemistry-Paraffin-NBP2-20418-img0004.jpg)
![Western Blot: SMC3 Antibody [NBP2-20418] - Sample (30 ug of whole cell lysate) A: NIH-3T3 B: JC C: BCL-1 5% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/SMC3-Antibody-Western-Blot-NBP2-20418-img0001.jpg)
![Western Blot: SMC3 Antibody [NBP2-20418] - Sample (30 ug of whole cell lysate) A: PC-12 5% SDS PAGE gel, diluted at 1:2000.](http://images.novusbio.com/fullsize/SMC3-Antibody-Western-Blot-NBP2-20418-img0002.jpg)
![Immunoprecipitation: SMC3 Antibody [NBP2-20418] - Immunoprecipitation of SMC3 protein from HeLa whole cell extracts using 5 ug of SMC3 antibody. Western blot analysis was performed using SMC3 antibody diluted at 1:500.](http://images.novusbio.com/fullsize/SMC3-Antibody-Immunoprecipitation-NBP2-20418-img0007.jpg)
![Western Blot: SMC3 Antibody (8W9R7) [NBP3-15798] - Western blot analysis of extracts of various cell lines, using SMC3 Rabbit mAb (NBP3-15798) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/SMC3-Antibody-8W9R7-Western-Blot-NBP3-15798-img0004.jpg "Western Blot: SMC3 Antibody (8W9R7) [NBP3-15798] - Western blot analysis of extracts of various cell lines, using SMC3 Rabbit mAb (NBP3-15798) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Immunocytochemistry/Immunofluorescence: SMC3 Antibody (8W9R7) [NBP3-15798] - Immunofluorescence analysis of C6 using SMC3 antibody (NBP3-15798) at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/SMC3-Antibody-8W9R7-Immunocytochemistry-Immunofluorescence-NBP3-15798-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: SMC3 Antibody (8W9R7) [NBP3-15798] - Immunofluorescence analysis of U2OS using SMC3 antibody (NBP3-15798) at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/SMC3-Antibody-8W9R7-Immunocytochemistry-Immunofluorescence-NBP3-15798-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: SMC3 Antibody (8W9R7) [NBP3-15798] - Immunofluorescence analysis of NIH/3T3 using SMC3 antibody (NBP3-15798) at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/SMC3-Antibody-8W9R7-Immunocytochemistry-Immunofluorescence-NBP3-15798-img0002.jpg)
![Immunoprecipitation: SMC3 Antibody (8W9R7) [NBP3-15798] - Immunoprecipitation analysis of 300ug extracts of U-87MG cells using 3ug SMC3 antibody (NBP3-15798). Western blot was performed from the immunoprecipitate using SMC3 antibody (NBP3-15798) at a dilition of 1:1000.](http://images.novusbio.com/fullsize/SMC3-Antibody-8W9R7-Immunoprecipitation-NBP3-15798-img0005.jpg)
![Western Blot: SMC3 Antibody [NBP1-88715] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: U-251 MG](http://images.novusbio.com/fullsize/SMC3-Antibody-Western-Blot-NBP1-88715-img0004.jpg "Western Blot: SMC3 Antibody [NBP1-88715] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: U-251 MG")
![Immunohistochemistry: SMC3 Antibody [NBP1-88715] - Staining of human smooth muscle shows strong cytoplasmic positivity in smooth muscle cells.](http://images.novusbio.com/fullsize/SMC3-Antibody-Immunohistochemistry-NBP1-88715-img0003.jpg)
![Simple Western: SMC3 Antibody [NBP1-88715] - Simple Western lane view shows a specific band for SMC3 in 0.2 mg/ml of U-251 MG lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation systems.](http://images.novusbio.com/fullsize/SMC3-Antibody-Simple-Western-NBP1-88715-img0005.jpg)
![Simple Western: SMC3 Antibody [NBP1-88715] - Electropherogram image of the corresponding Simple Western lane view. SMC3 antibody was used at 1:25 dilution on U-251 MG lysate(s) respectively.](http://images.novusbio.com/fullsize/SMC3-Antibody-Simple-Western-NBP1-88715-img0006.jpg)
![Western Blot: SMC3 [p Ser103] Antibody [NBP2-37694] - Detection of human Phospho SMC3 (S1083) by western blot (WB). Samples: Whole cell lysate (50 ug) from HeLa cells treated with 100 uM EPE and/or 100 uM phosphatase for 4 hours (+) or mock treated (-) cells. Antibodies: Affinity purified rabbit anti-Phospho SMC3 (S1083) antibody NBP2-37694 used for WB at 1 ug/ml (upper panel); to detect total SMC3, another rabbit anti-SMC3 was used for WB at 0.1 ug/ml (lower panel). Detection: Chemiluminescence with an exposure time of 3 minutes (upper panel) or 10 seconds (lower panel).](http://images.novusbio.com/fullsize/SMC3-[p-Ser103]-Antibody-Western-Blot-NBP2-37694-img0006.jpg "<b>Independent Antibodies Validation. </b>Western Blot: SMC3 [p Ser103] Antibody [NBP2-37694] - Detection of human Phospho SMC3 (S1083) by western blot (WB). Samples: Whole cell lysate (50 ug) from HeLa cells treated with 100 uM EPE and/or 100 uM phosphatase for 4 hours (+) or mock treated (-) cells. Antibodies: Affinity purified rabbit anti-Phospho SMC3 (S1083) antibody NBP2-37694 used for WB at 1 ug/ml (upper panel); to detect total SMC3, another rabbit anti-SMC3 was used for WB at 0.1 ug/ml (lower panel). Detection: Chemiluminescence with an exposure time of 3 minutes (upper panel) or 10 seconds (lower panel).")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: SMC3 [p Ser103] Antibody [NBP2-37694] - Whole cell lysate (50 ug) from HeLa cells treated with 500 uM EPE for 4 hours (+) or mock treated (-) cells Affinity purified rabbit anti-Phospho SMC3 (S1083) antibody used for WB at 1 ug/ml (upper panel); to detect total SMC3, rabbit anti-SMC3 was used for WB at 0.1 ug/ml (lower panel). Detection: Chemiluminescence with an exposure time of 3 minutes (upper panel) or 30 seconds (lower panel).](http://images.novusbio.com/fullsize/SMC3-[p-Ser103]-Antibody-Western-Blot-NBP2-37694-img0002.jpg)
![<b>Biological Strategies Validation. </b>Immunoprecipitation: SMC3 [p Ser103] Antibody [NBP2-37694] - Whole cell lysate (1mg for IP; 20% of IP loaded) from HeLa cells treated with 500 uM EPE for 4 hours (+). Antibodies: Phospho SMC3 was immunoprecipitated (IP) using rabbit anti-Phospho SMC3 (S1083) antibody. The IP reactions were either mock treated (-) or treated with phosphatases (+). Affinity purified rabbit anti-Phospho SMC3 (S1083) antibody was used for western blot (WB) of IPs at 1 ug/ml (upper panel); to detect total SMC3 affinity purified rabbit anti-SMC3 was used for WB of IPs at 0.1 ug/ml (lower panel). Detection: Chemiluminescence with an exposure time of 3 minutes (upper panel) and 30 seconds (lower panel).](http://images.novusbio.com/fullsize/SMC3-[p-Ser103]-Antibody-Immunoprecipitation-NBP2-37694-img0004.jpg)
![SMC3 [p Ser103] Antibody [NBP2-37694] - Detection of human Phospho SMC3 (S1083) by western blot of immunoprecipitates. Samples: Whole cell lysate (1mg for IP; 20% of IP loaded) from HeLa cells treated with 100 uM EPE for 4 hours (+) or mock treated (-). Antibodies: Phospho SMC3 (S1083) was immunoprecipitated using rabbit anti-Phospho SMC3 (S1083) antibody NBP2-37694 at 6 ug per reaction. Total SMC3 was immunoprecipitated with another rabbit anti-SMC3 antibody. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/SMC3-[p-Ser103]-Antibody-NBP2-37694-img0005.jpg)
![Western Blot: SMC3 Antibody [NBP2-58330] - Analysis in Rh30 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-SMC3 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/SMC3-Antibody-Western-Blot-NBP2-58330-img0002.jpg "<b>Genetic Strategies Validation. </b>Western Blot: SMC3 Antibody [NBP2-58330] - Analysis in Rh30 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-SMC3 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: SMC3 Antibody [NBP2-58330] - Staining of human cell line U-2 OS shows localization to nucleoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/SMC3-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-58330-img0001.jpg)
![Immunoprecipitation: SMC3 [p Ser383] Antibody [NB100-208] - Immunoprecipitation of Human Phospho SMC3 (Ser383). Samples: Nuclear extracts (50 ug) from HeLa cells that were irradiated with 10 Gy of ionizing radiation or mock irradiated. Antibodies: Rabbit anti-SMC3 pSer383 NB100-208 was used at 4 ug/mg lysate to IP phospho SMC3 (Ser383). Rabbit anti-SMC3 NB100-208 was used at 4 ug/mg lysate for IP and at 0.5 ug/ml for WB. Detection: Chemiiluminescence.](http://images.novusbio.com/fullsize/SMC3-[p-Ser383]-Antibody-Immunoprecipitation-NB100-208-img0001.jpg "Immunoprecipitation: SMC3 [p Ser383] Antibody [NB100-208] - Immunoprecipitation of Human Phospho SMC3 (Ser383). Samples: Nuclear extracts (50 ug) from HeLa cells that were irradiated with 10 Gy of ionizing radiation or mock irradiated. Antibodies: Rabbit anti-SMC3 pSer383 NB100-208 was used at 4 ug/mg lysate to IP phospho SMC3 (Ser383). Rabbit anti-SMC3 NB100-208 was used at 4 ug/mg lysate for IP and at 0.5 ug/ml for WB. Detection: Chemiiluminescence.")
![Western Blot: SMC3 Antibody [NBP3-35831] - Western blot analysis of various lysates using SMC3 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.](http://images.novusbio.com/fullsize/nbp3-35831_rabbit-smc3-pab-91220241519596.jpg "Western Blot: SMC3 Antibody [NBP3-35831] - Western blot analysis of various lysates using SMC3 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.")
![Immunoprecipitation: SMC3 Antibody [NBP3-35831] - Immunoprecipitation analysis of 600 ug extracts of Mouse spleen using 3 ug SMC3 antibody. Western blot was performed from the immunoprecipitate using SMC3 antibody at a dilution of 1:1000.](http://images.novusbio.com/fullsize/nbp3-35831_rabbit-smc3-pab-912202414112882.jpg)
![Western Blot: SMC3 Antibody [NBP1-19109] - Whole cell lysate from HeLa (5, 15 and 50 ug), 293T (T; 50 ug) and mouse NIH3T3 (M; 50ug) cells. Antibody used at for WB at 0.04 ug/ml.](http://images.novusbio.com/fullsize/SMC3-Antibody-Western-Blot-NBP1-19109-img0002.jpg "<b>Biological Strategies Validation. </b>Western Blot: SMC3 Antibody [NBP1-19109] - Whole cell lysate from HeLa (5, 15 and 50 ug), 293T (T; 50 ug) and mouse NIH3T3 (M; 50ug) cells. Antibody used at for WB at 0.04 ug/ml.")
![Western Blot: SMC3 Antibody (2F11) [H00009126-M02] - Analysis of SMC3 expression in transfected 293T cell line by CSPG6 monoclonal antibody (M02), clone 2F11.Lane 1: SMC3 transfected lysate(141.5 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/SMC3-Antibody-2F11-Western-Blot-H00009126-M02-img0005.jpg "Western Blot: SMC3 Antibody (2F11) [H00009126-M02] - Analysis of SMC3 expression in transfected 293T cell line by CSPG6 monoclonal antibody (M02), clone 2F11.Lane 1: SMC3 transfected lysate(141.5 KDa).Lane 2: Non-transfected lysate.")
![Sandwich ELISA: SMC3 Antibody (2F11) [H00009126-M02] - Detection limit for recombinant GST tagged CSPG6 is approximately 0.3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/SMC3-Antibody-2F11-Sandwich-ELISA-H00009126-M02-img0004.jpg)