SMC3 [p Ser383] Antibody - BSA Free

Immunoprecipitation: SMC3 [p Ser383] Antibody [NB100-208] - Immunoprecipitation of Human Phospho SMC3 (Ser383). Samples: Nuclear extracts (50 ug) from HeLa cells that were irradiated with 10 Gy of ionizing radiation or mock irradiated. Antibodies: Rabbit anti-SMC3 pSer383 NB100-208 was used at 4 ug/mg lysate to IP phospho SMC3 (Ser383). Rabbit anti-SMC3 NB100-208 was used at 4 ug/mg lysate for IP and at 0.5 ug/ml for WB. Detection: Chemiiluminescence.

• Reactivity: Hu
• Applications: WB, ICC/IF, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

SMC3 [p Ser383] Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit SMC3 [p Ser383] Antibody - BSA Free (NB100-208) is a polyclonal antibody validated for use in WB, ICC/IF and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Immunogen for this antibody was a phosphorylated synthetic peptide, which represented a portion of human Structural Maintenance of Chromosomes 3 (GeneID 9126) around serine 383 according to the numbering given in entry NP_005436.1.
• Modification: p Ser383
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SMC3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunoprecipitation 2 - 6 ug/mg of lysate
  • Western Blot

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for SMC3 [p Ser383] Antibody - BSA Free

• Bamacan
• BAMSMC protein 3
• Basement membrane-associated chondroitin proteoglycan
• BMH
• chondroitin sulfate proteoglycan 6 (bamacan)
• Chondroitin sulfate proteoglycan 6
• Chromosome-associated polypeptide
• CSPG6structural maintenance of chromosomes protein 3
• hCAP
• HCAPbamacan
• SMC-3
• SMC3L1CDLS3
• structural maintenance of chromosomes 3

Background

Involved in chromosome cohesion during cell cycle and in DNA repair. Central component of cohesin complex. The cohesin complex is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis and in chromosome movement.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SMC3
• Entrez:
  • 9126(Human)
• Uniprot:
  • NP_005436.1.(Human)