SMC1 [p Ser957] Antibody - BSA Free

Western Blot: SMC1 [p Ser957] Antibody [NB100-205] - Whole cell extracts from HeLa cells (30 ug/lane) that were irradiated with 10 Gy of ionizing radiation or mock irradiated. Irradiated lysate was then treated with calf intestinal phosphatase (CIP) or left untreated. Antibody used at 0.1 ug/ml.

SMC1 [p Ser957] Antibody [NB100-205] - Whole cell lysate (50 ug) from 293T cells treated with 100 uM EPE for 4 hours (+) or mock treated (-) cells. Antibody: Affinity purified rabbit antiPhospho SMC1 (S957) antibody used for WB at 0.5 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Product Discontinued

SMC1 [p Ser957] Antibody - BSA Free Summary

• Immunogen: Immunogen for this antibody was a phosphorylated synthetic peptide, which represented a portion of human Structural Maintenance of Chromosomes 1 (GeneID 8243) around serine 957 according to the numbering given in entry (NP_006297.2)
• Modification: p Ser957
• Predicted Species: Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SMC1A
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 2 - 6 ug/mg of lysate
  • Western Blot 1:200-1:2
• Theoretical MW: 143 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  SMC1 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for SMC1 [p Ser957] Antibody - BSA Free

• CDLS2
• DXS423ESMC1 structural maintenance of chromosomes 1-like 1 (yeast)
• KIAA0178DKFZp686L19178
• SB1.8
• SB1.8MGC138332
• segregation of mitotic chromosomes 1
• SMC protein 1A
• SMC1 (structural maintenance of chromosomes 1, yeast)-like 1
• SMC1 structural maintenance of chromosomes 1-like 1
• SMC1
• SMC1A
• SMC-1A
• SMC-1-alpha
• SMC1L1
• SMC1SMC1alpha
• Smcb
• structural maintenance of chromosomes 1A
• structural maintenance of chromosomes protein 1A

Background

Involved in chromosome cohesion during cell cycle and in DNA repair and is a central component of cohesin complex. The cohesin complex is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis. SMC1 is involved in DNA repair via its interaction with BRCA1 and its related phosphorylation by ATM, and works as a downstream effector in the ATM/NBS1 branch of S-phase checkpoint.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SMC1 Antibody (NB100-205)(17)

Publications using NB100-205

• Valdez BC, Li Y, Murray D et al. Comparison of the cytotoxicity of cladribine and clofarabine when combined with fludarabine and busulfan in AML cells: Enhancement of cytotoxicity with epigenetic modulators. Exp Hematol 2015-06-01 [PMID: 25704054]
• Nayler S, Gatei M, Kozlov S et al. Embryonic Stem Cells/Induced Pluripotent Stem (iPS) Cells: Induced Pluripotent Stem Cells from Ataxia-Telangiectasia Recapitulate the Cellular Phenotype Stem Cells Transl Med 2012-12-01 [PMID: 23197857]
• Valdez BC, Nieto Y, Murray D et al. Epigenetic modifiers enhance the synergistic cytotoxicity of combined nucleoside analog-DNA alkylating agents in lymphoma cell lines. Exp Hematol 2012-10-01 [PMID: 22687754] (WB, Human)
• Williamson, C T, Kubota, E, Hamill, J D et al. Enhanced cytotoxicity of PARP inhibition in mantle cell lymphoma harbouring mutations in both ATM and p53 EMBO Mol Med. 2012-03-27 [PMID: 22416035] (WB, Human)
• Valdez BC, Murray D, Nieto Y et al. Synergistic cytotoxicity of the DNA alkylating agent busulfan, nucleoside analogs and suberoylanilide hydroxamic acid in lymphoma cell lines. Leuk Lymphoma 2012-05-01 [PMID: 22023523] (Human)
• Valdez BC, Li Y, Murray D et al. The synergistic cytotoxicity of clofarabine, fludarabine and busulfan in AML cells involves ATM pathway activation and chromatin remodeling. Biochem Pharmacol 2011-01-15 [PMID: 20933509] (WB, Human)
• Williamson CT, Muzik H, Turhan AG et al. ATM deficiency sensitizes mantle cell lymphoma cells to poly(ADP-ribose) polymerase-1 inhibitors. Mol Cancer Ther 2010-02-01 [PMID: 20124459]
• Kodama M, Otsubo C, Hirota T et al. Requirement of ATM for rapid p53 phosphorylation at Ser46 without Ser/Thr-Gln sequences. Mol Cell Biol 2010-04-01 [PMID: 20123963]
• Li T, Chanda D, Zhang Y et al. Glucose stimulates cholesterol 7alpha-hydroxylase gene transcription in human hepatocytes. J Lipid Res 2010-04-01 [PMID: 19965590] (Human)
• Pennarun G, Granotier C, Hoffschir F et al. Role of ATM in the telomere response to the G-quadruplex ligand 360A. Nucleic Acids Res;36(5):1741-54. 2008-03-01 [PMID: 18263609]
• Pereg Y, Lam S, Teunisse A et al. Differential roles of ATM- and Chk2-mediated phosphorylations of Hdmx in response to DNA damage. Mol Cell Biol 2006-09-01 [PMID: 16943424]
• Dar, I, Biton, S, Shiloh, Y, Barzilai, A. Analysis of the Ataxia Telangiectasia Mutated-Mediated DNA Damage Response in Murine Cerebellar Neurons, . 26, 7767-7774. 2006-01-01 [PMID: 16855104]
• Biton S, Dar I, Mittelman L et al. Nuclear ataxia-telangiectasia mutated (ATM) mediates the cellular response to DNA double strand breaks in human neuron-like cells. J Biol Chem 2006-06-01 [PMID: 16627474]
• Bekker-Jensen, S, Lukas, C, Kitagawa, R, Meler, F, Kastan, M B, Bartek, J, Lukas, J. Spatial organization of the mammalian genome surveillance machinery in response to DNA str breaks, . 173, 195-206. 2006-01-01 [PMID: 16618811]
• Goodarzi, A et al. Autophosphorylation of ataxia-telangiectasia mutated is regulated by protein phosphatase 2A. The EMBO Journal 23: 4451-4461. 2004-01-01 [PMID: 15510216]
• Kurz, E et al. Doxorubicin Activates ATM-dependent Phosphorylation of Multiple Downstream Targets in Part through the Generation of Reactive Oxygen Species. The Journal of Biological Chemistry 279(51): 53272-53281. 2004-01-01 [PMID: 15489221]
• Wang, J et al. ATM-dependent CHK2 Activation Induced by Anticancer Agent, Irofulven. J Biol Chem 279:39584-39592. 2004-01-01 [PMID: 15269203]

FAQs for SMC1 Antibody (NB100-205). (Showing 1 - 2 of 2 FAQs).

1. I would like to use one of your anti-smc1 antibodies to detect smc1 in an invertebrate. Your different antibodies are raised against different peptides derived from different regions possibly well-conserved, but not completely, in the organism that interests me (Schistosoma mansoni). Would it be possible to know the sequence of the different peptides that was used to generate the different antibodies, or failing that, could you analyze the corresponding sequence in my species of interest in order to determine whether or not it corresponds to one of the different peptides?
   • My suggestion to you would be to look at the homology between the protein expressed in your species and the protein expressed in the reactive species of the antibodies. A homology of >90% is a great start, >80% is acceptable. Please view our list of smc1 antibodies we currently offer and there are 27 products.
2. My sequence of interest have >80% homology with the N-term and the C-term parts of human smc1. Your antibodies NB100-78322 and NB100-204 recognized the N-term and C-term of human smc1 respectively, therefore it’s possible that the cross reactivity can happen. I would be very interested to test these antibodies (NB100-78322 and NB100-204). Can you provide me free sample (few ul) to do western blot. I will share the testing result with you.
   • We do not provide free samples for testing. If you are interested in testing these antibodies in an unlisted species or application, you would qualify for the Innovator's Rewards program. The way the program works is that you would purchase the antibody of interest, perform your testing using appropriate controls and then share your data with us. Upon review of your data we would issue you a voucher for a free antibody. You can find more details here.

Bioinformatics

• Gene Symbol: SMC1A
• Entrez:
  • 8243(Human)
• Uniprot:
  • Q14683()