SMARCD3 Antibody (5F12) - Azide and BSA Free

Western Blot: SMARCD3 Antibody (5F12) [H00006604-M02] - SMARCD3 monoclonal antibody (M02), clone 5F12. Analysis of SMARCD3 expression in NIH/3T3.

Western Blot: SMARCD3 Antibody (5F12) [H00006604-M02] - SMARCD3 monoclonal antibody (M02), clone 5F12 Analysis of SMARCD3 expression in K-562.

Sandwich ELISA: SMARCD3 Antibody (5F12) [H00006604-M02] - Detection limit for recombinant GST tagged SMARCD3 is approximately 0.3ng/ml as a capture antibody.

• Reactivity: Hu, Mu
• Applications: WB, ELISA
• Clone: 5F12
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

SMARCD3 Antibody (5F12) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: SMARCD3 (NP_001003801, 385 a.a. ~ 483 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. ISALDSKIHETIESINQLKIQRDFMLSFSRDPKGYVQDLLRSQSRDLKVMTDVAGNPEEERRAEFYHQPWSQEAVSRYFYCKIQQRRQELEQSLVVRNT
• Specificity: SMARCD3 - SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily d, member 3 (5F12)
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: SMARCD3
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Sandwich ELISA
  • Western Blot
• Application Notes: Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for ELISA.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for SMARCD3 Antibody (5F12) - Azide and BSA Free

• 60 kDa BRG-1/Brm-associated factor subunit C
• 60kDa BRG-1/Brm associated factor subunit c
• BAF60Cchromatin remodeling complex BAF60C subunit
• BRG1-associated factor 60C
• CRACD3
• mammalian chromatin remodeling complex BRG1-associated factor 60C
• MGC111010
• Rsc6p
• subfamily d, member 3
• SWI/SNF complex 60 kDa subunit C
• SWI/SNF related, matrix associated, actin dependent regulator of chromatin
• SWI/SNF-related matrix-associated actin-dependent regulator of chromatinsubfamily D member 3
• Swp73-like protein

Background

The protein encoded by this gene is a member of the SWI/SNF family of proteins, whose members display helicase and ATPase activities and which are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI and has sequence similarity to the yeast Swp73 protein. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SMARCD3
• Entrez:
  • 6604(Human)
• Uniprot:
  • NP_001003801(Human)