SmarcAL1 Antibody (PCRP-SMARCAL1-1C4)

Analysis of Protein Array containing more than 19,000 full-length human proteins using SMARCAL1 Mouse Monoclonal Antibody (PCRP-SMARCAL1-1C4)

Formalin-fixed, paraffin-embedded human tonsil stained with SmarcAL1 antibody (PCRP-SMARCAL1-1C4). Inset: PBS instead of primary antibody; secondary only negative control.

Formalin-fixed, paraffin-embedded human kidney carcinoma stained with SmarcAL1 antibody (PCRP-SMARCAL1-1C4). HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.

• Reactivity: Hu
• Applications: IHC
• Clone: PCRP-SMARCAL1-1C4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

SmarcAL1 Antibody (PCRP-SMARCAL1-1C4) Summary

• Description: Positive Controls: HeLa whole cell lysate. Human prostate or breast carcinoma.
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Non-hazardous. No MSDS required.
• Immunogen: Recombinant full-length human SmarcAL1 protein
• Localization: Nucleus.
• Isotype: IgG2c Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: SMARCAL1
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry-Paraffin 1-2 ug/ml
• Application Notes: Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes. at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
  
  Optimal dilution for a specific application should be determined.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: 10mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A or G purified

Alternate Names for SmarcAL1 Antibody (PCRP-SMARCAL1-1C4)

• ATP-driven annealing helicase
• EC 3.6.1
• EC 3.6.4.-
• HARPSWI/SNF-related matrix-associated actin-dependent regulator of chromatinsubfamily A-like protein 1
• HepA-related protein
• HHARP
• SIOD
• SMARCA-like protein 1
• subfamily a-like 1
• Sucrose nonfermenting protein 2-like 1
• SWI/SNF related, matrix associated, actin dependent regulator of chromatin

Background

SmarcAL1 is encoded by this gene is a member of the SWI/SNF family of proteins. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein shows sequence similarity to the E. coli RNA polymerase-binding protein HepA. Mutations in this gene are a cause of Schimke immunoosseous dysplasia (SIOD), an autosomal recessive disorder with the diagnostic features of spondyloepiphyseal dysplasia, renal dysfunction, and T-cell immunodeficiency.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SMARCAL1
• Uniprot:
  • Q9NZC9(Human)