SMARCA6 Antibody - BSA Free

Western Blot: SMARCA6 Antibody [NBP3-03793] - Analysis of extracts of various cell lines, using SMARCA6 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit.

Western Blot: SMARCA6 Antibody [NBP3-03793] - Analysis of extracts from normal (control) and HELLS knockout (KO) HeLa cells, using SMARCA6 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Immunoprecipitation: SMARCA6 Antibody [NBP3-03793] - Analysis of 200ug extracts of 293T cells using SMARCA6 antibody . Western blot was performed from the immunoprecipitate using this antibody.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IP, KO
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

SMARCA6 Antibody - BSA Free Summary

• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 719-838 of human SMARCA6 (NP_060533.2). VYRLVTANTIDQKIVERAAAKRKLEKLIIHKNHFKGGQSGLNLSKNFLDPKELMELLKSRDYEREIKGSREKVISDKDLELLLDRSDLIDQMNASGPIKEKMGIFKILENSEDSSPECLF
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: HELLS
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 1:50 - 1:200
  • Knockout Validated
  • Western Blot 1:500 - 1:2000

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for SMARCA6 Antibody - BSA Free

• EC 3.6.1
• EC 3.6.4.-
• helicase, lymphoid-specific
• LSHSWI/SNF2-related, matrix-associated, actin-dependent regulator of chromatin
• Nbla10143
• PASGFLJ10339
• Proliferation-associated SNF2-like protein
• SMARCA6lymphoid-specific helicase
• subfamily A, member 6
• SWI/SNF2-related matrix-associated actin-dependent regulator of chromatinsubfamily A member 6

Background

HELLS, also known as LSH (lymphoid specific helicase), is a member of the SNF2 superfamily of chromatin helicases. HELLS is ubiquitously expressed and is required for the maintenance of normal DNA methylation patterns important to organism development. HELLS function has also been shown to be important to the regulation of proliferation and the expression of senescence-related genes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HELLS