Smad4 Antibody (SP05-05)

Western Blot: Smad4 Antibody (SP05-05) [NBP2-67372] - Western blot analysis of Smad4 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rab

Immunocytochemistry/Immunofluorescence: Smad4 Antibody (SP05-05) [NBP2-67372] - Staining Smad4 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: Smad4 Antibody (SP05-05) [NBP2-67372] - Analysis of paraffin-embedded mouse colon tissue using anti-Smad4 antibody. Counter stained with hematoxylin.

Flow Cytometry: Smad4 Antibody (SP05-05) [NBP2-67372] - Analysis of SH-SY-5Y cells with Smad4 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

Western Blot: Smad4 Antibody (SP05-05) [NBP2-67372] - Analysis of Smad4 on PC-12 cells lysates using anti-Smad4 antibody at 1/1,000 dilution.

Immunocytochemistry/Immunofluorescence: Smad4 Antibody (SP05-05) [NBP2-67372] - Staining Smad4 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: Smad4 Antibody (SP05-05) [NBP2-67372] - Staining Smad4 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: Smad4 Antibody (SP05-05) [NBP2-67372] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-Smad4 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Smad4 Antibody (SP05-05) [NBP2-67372] - Analysis of paraffin-embedded human lung cancer tissue using anti-Smad4 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Smad4 Antibody (SP05-05) [NBP2-67372] - Analysis of paraffin-embedded mouse brain tissue using anti-Smad4 antibody. Counter stained with hematoxylin.

Product Discontinued

Smad4 Antibody (SP05-05) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within C-terminal human Smad4. (SwissProt: Q13485 Human; SwissProt: P97471 Mouse; SwissProt: O70437 Rat)
• Localization: Cytoplasm, Nucleus.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: SMAD4
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Smad4 Antibody (SP05-05)

• DPC4
• DPC4SMAD, mothers against DPP homolog 4 (Drosophila)
• hSMAD4
• JIP
• MAD homolog 4
• MADH4
• MADH4mothers against decapentaplegic homolog 4
• mothers against decapentaplegic homolog 4
• mothers against decapentaplegic, Drosophila, homolog of, 4
• SMAD 4
• SMAD family member 4MAD, mothers against decapentaplegic homolog 4 (Drosophila)
• SMAD, mothers against DPP homolog 4
• Smad4

Background

Smad proteins, the mammalian homologs of the Drosophila Mothers against dpp (Mad) have been implicated as downstream effectors of TGFbeta/BMP signaling. Smad1 (also designated Madr1 or JV4-1), Smad5 and mammalian Smad8 (also designated Smad9 or MadH6) are effectors of BMP2 and BMP4 function while Smad2 (also designated Madr2 or JV18-1) and Smad3 are involved in TGFbeta and activin-mediated growth modulation. Smad4 (also designated DPC4) has been shown to mediate all of the above activities through interaction with various Smad family members. Smad6 and Smad7 regulate the response to activin/TGFbeta signaling by interfering with TGFbeta-mediated phosphorylation of other Smad family members.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Smad4 Antibody (NBP2-67372)(1)

Publication using NBP2-67372

• Mukhi D, Kolligundla LP, Maruvada S et al. Growth hormone induces transforming growth factor-beta 1 in podocytes: Implications in podocytopathy and proteinuria Biochimica et biophysica acta. Molecular cell research 2022-11-15 [PMID: 36400249] (ICC/IF, Human)

Bioinformatics

• Gene Symbol: SMAD4