Smad2 [p Ser250] Antibody (S02-0D1)

Western Blot: Smad2 [p Ser250] Antibody (S02-0D1) [NBP3-19828] - Western blot detection of Smad2 (phospho-Ser250) in Hela, CHO-K1, C6 using NBP3-19828 (1:1000 diluted)

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clone: S02-0D1
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.3 mg/ml

Smad2 [p Ser250] Antibody (S02-0D1) Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthetic phosphopeptide corresponding to residues surrounding Ser250 of human Smad2 (Uniprot # Q15796)
• Modification: p Ser250
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: SMAD2
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1/1000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA
• Preservative: 0.01% Sodium Azide
• Concentration: 0.3 mg/ml
• Purity: Affinity purified

Alternate Names for Smad2 [p Ser250] Antibody (S02-0D1)

• hMAD-2
• hSMAD2
• JV18
• JV18-1
• JV18-1SMAD, mothers against DPP homolog 2 (Drosophila)
• MAD homolog 2
• Mad protein homolog
• Mad, mothers against decapentaplegic homolog 2
• MADH2
• MADH2MGC34440
• MADR2
• MADR2MGC22139
• Mad-related protein 2
• mother against DPP homolog 2
• mothers against decapentaplegic homolog 2
• Mothers against DPP homolog 2
• Sma- and Mad-related protein 2
• SMAD 2
• SMAD family member 2MAD, mothers against decapentaplegic homolog 2 (Drosophila)
• SMAD, mothers against DPP homolog 2
• Smad2
• Smox

Background

Smad 2 is a member of the Mothers Against Dpp (MAD)-related family of proteins. So far, nine Smads have been identified and can be divided in 3 subgroups based on their structure and functions; pathway-restricted, common mediator and inhibitory Smad. Smad 2 and 3 serve as pathway-restricted Smads for the TGF-beta/activin signaling pathways (1-2). Smad 2 is phosphorylated by the TGF-beta type 1 receptor kinase on Ser 465 and Ser 467. Phosphorylation of both serines is essential for Smad 2-Smad4 heteromeric complex and dissociation of Smad 2 from the TGF-beta type 1 receptor (3). Once phosphorylated Smad2 translocate to the nucleus where it associates with DNA-binding proteins and forming a transcriptional complex (4). Unlike Smad 3 and 4, Smad 2 does not directly bind DNA (5).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SMAD2