SMAC/Diablo Antibody

Product Discontinued

SMAC/Diablo Antibody Summary

• Immunogen: Synthetic peptide corresponding to the C terminus of the human DIABLO protein.
• Specificity: Reacts with the C terminus of the human DIABLO protein.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: DIABLO
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500 -1:1000
• Application Notes: Western blot- Use at 1:500 to 1:1,000 dilution. However, the investigator should determine the optimal dilution for a specific application such as immunohistochemistry.

Reactivity Notes

Human. Other species not tested.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for SMAC/Diablo Antibody

• 0610041G12Rik
• Diablo
• diablo, IAP-binding mitochondrial protein
• Direct IAP-binding protein with low pI
• mitochondrial Smac protein
• Second mitochondria-derived activator of caspase
• SMAC
• SMACmitochondrial

Background

DIABLO (direct IAP binding protein with low pI) is a novel protein that can bind mammalian IAP homolog A (MIHA, also known as XIAP), and can also interact with MIHB and MIHC and the baculoviral IAP, OpIAP. DIABLO may promote apoptosis by binding to IAPs and preventing them from inhibiting caspases.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SMAC/Diablo Antibody (NB500-226)(1)

Publication using NB500-226

• Sikorski K, Mehta A et al. A high-throughput pipeline for validation of antibodies. Nat Methods 2018-01-11 [PMID: 30377371] (Human)
  
  Details:
  Antibody validation based on denaturing gel electrophoresis of biotinylated cell lysates (PAGE) followed by mass spectrometry (MS) and antibody array analysis (MAP).

Bioinformatics

• Gene Symbol: DIABLO
• Entrez:
  • 56616(Human)